Phenotypic Analyses and Identification of Causal Mutations In Medaka Mutants with Gonadal Dysgenesis.

性腺发育不全青鳉突变体的表型分析和因果突变鉴定。

• 批准号: 17370083
• 负责人: TANAKA Minoru
• 金额: $ 8.13万
• 依托单位: National Institute for Basic Biology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17370083/
• 关键词: SEX DIFFERENTIATION; GONAD; MUTANT; MEDAKA; AMH; CELL LINEAGE; PHENOTYPE; SEX REVERSAL; マッピング; 卵巣

Before analysis of the mutants displaying defects in gonadogenesis, the cell lineages that constitute the gonad have been characterized in terms of the motility, origin and translational regulation. Then, phenotypic analyses and identification of the mutations have been performed for the mutant medaka.Mutant medaka, totoro (tot), was identified as medaka showing a hypertrophic gonad. Phenotypic analyses showed that tot possessed an excessive number of germ cells and a half of genetic male exhibited male-to-female sex reversal. A mutation was found in the kinase domain of anti-Mullerian hormone receptor type II, resulting in tyrosine to cysteine substitution. This gene has been known to be involved in sex-specific development of accessory organs found in only mammals and function in other vertebrates remained to be elucidated. Our result indicated that this gene is involved in proliferation of germ cells in a sex-dependent manner. Since sex-reversal was speculated to be due to a result of a burst number of germ cells, medaka with the complete loss of germ cells were generated. The germ-cell deficient medaka displayed a female-male sex reversal, suggesting that germ cells are deeply committed to sexual dimorphic gonadogenesis.A mutation of zenzai mutant has also been identified in one of the surface antigen genes. In vivo germ cell-tracing analysis indicated that two types of proliferation are present in the gonads and the process of proliferation involved in germ cell maintenance was affected in this mutant, which brings about sterility in adult stage.

在分析性腺发生缺陷的突变体之前，构成性腺的细胞谱系已经从运动性、起源和翻译调节方面进行了表征。然后，对突变的青竹进行了表型分析和突变鉴定。突变的青竹，龙猫(TOT)被鉴定为青竹，显示出性腺肥大。表型分析表明，TOT生殖细胞数量过多，有一半的遗传雄性发生了雌雄逆转。在抗苗勒氏激素受体II型的激动区发现突变，导致酪氨酸替换为半胱氨酸。已知该基因参与了仅在哺乳动物中发现的辅助器官的性别特异性发育，而在其他脊椎动物中的功能仍未阐明。我们的结果表明，该基因以性别依赖的方式参与生殖细胞的增殖。由于性反转被推测是生殖细胞数量激增的结果，因此产生了生殖细胞完全丧失的青竹。生殖细胞缺陷的青竹表现出雌雄反转，这表明生殖细胞深深地致力于性二型性发育。在其中一个表面抗原基因中也发现了zenzai突变。体内生殖细胞示踪分析表明，该突变体在性腺中存在两种类型的增殖，并影响了生殖细胞维持所涉及的增殖过程，导致成年后不育。

项目成果

Visualization of primordial germ cells in vivo using GFP-nos1 3′UTR mRNA

• DOI: 10.1387/ijdb.062143ts
• 发表时间: 2006-01-01
• 期刊: INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY
• 影响因子: 0.7
• 作者: Saito, Taiju;Fujimoto, Takafumi;Yamaha, Etsuro
• 通讯作者: Yamaha, Etsuro

Sox9b/sox9a2-EGFP transgenic medaka reveals the morphological reorganization of the gonads and a common presursor of both the female and male supporting cells.

Sox9b/sox9a2-EGFP 转基因青鳉揭示了性腺的形态重组以及雌性和雄性支持细胞的共同前体。

• 发表时间: 2007
• 期刊: Mol. Reprod. Dev.
• 作者: Nakamura;S.;Aoki;Y.;Saito;D.;Kuroki;Y.;Fujiyama;A.;Naruse;K.;Tanaka;M.
• 通讯作者: M.

Identification and lineage tracing of two population of somatic gonadal precursors in medaka embryos.

青鳉胚胎中两个体细胞性腺前体群体的鉴定和谱系追踪。

• 发表时间: 2006
• 期刊: Dev. Biol. 295
• 作者: Imamura T;Paldi A;Takamautsu N. et al.;今村拓也;Nakamura S. et al.
• 通讯作者: Nakamura S. et al.

Quantitative bioimaging analysis of gonads in olvas-GFP/ST-II YI medaka (transgenic Oryzias latipes) exposed to ethinylestradiol

• DOI: 10.1021/es0620134
• 发表时间: 2007-02-15
• 期刊: ENVIRONMENTAL SCIENCE & TECHNOLOGY
• 影响因子: 11.4
• 作者: Hano, Takeshi;Oshima, Yuji;Honjo, Tsuneo
• 通讯作者: Honjo, Tsuneo

Sox9b/sox9a2-EGFP transgenic medaka reveals the orphological reorganization of the gonads and a common presursor of both the female and male supporting cells.

Sox9b/sox9a2-EGFP 转基因青鳉揭示了性腺的形态重组以及雌性和雄性支持细胞的共同前体。

• 发表时间: 2007
• 期刊: Mol. Reprod. Dev. (印刷中)
• 作者: 米井小百合;阿部玄武;田村宏治;Nakamura S. et al.
• 通讯作者: Nakamura S. et al.