Basic study for application of stress-resistant effect of prolactin to animals

催乳素在动物抗应激作用中应用的基础研究

• 批准号: 15380203
• 负责人: TANAKA Minoru
• 金额: $ 5.89万
• 依托单位: Nippon Veterinary and Animal Science University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15380203/
• 关键词: Prolactin; Prolactin receptor; Stress; Brain; Gene expression; Maternalbehavior; 不安傾向; 神経回路

(1)Effects of rearing with low-prolactin milk on the stress-resistance and maternal behavior of rats at adulthoodEffects of milk-born prolactin on the development of brain function were examined in rats. Bromocriptin, a prolactin secretion inhibitor, was administrated into intraperitoneal cavity at days 20 and 21 of pregnancy, and stress-tolerance and maternal behavior reared by the bromocriptin-administrated mothers were tested at adulthood. Both the extent of gastric ulcer caused by water-restraint stress and latency of maternal behavior to foster pups were significantly increased in the rats reared by the bromocriptin-administrated mothers compared to those in rats reared by control mothers. These results suggest that milk-born prolactin plays an important role in establishment of neural networks for stress tolerance and maternal behavior.(2)Identification of a novel first exon of rat prolactin receptor gene and regulation of the prolactin receptor gene expression in the liver by sex steroid hormonesIn addition to the known four first exones, E1-1,E1-2,E1-3 and E1-4, another first exon, E1-5,was identified in the rat prolactin receptor gene. Analysis of expression of each first exon in the liver revealed that sex steroid hormones specifically regulate the expression of E1-2 first exon(3)Mechanism of brain-specific regulation of prolactin receptor gene expression in the ratThe brain-specific exon 1,E1-4,was found to localize between exons 2 and 3 of rat prolactin receptor gene, and analysis of expression of each first exon in the brain revealed that induction of prolactin gene expression during development depends on the expression of E1-4.

（1）在大鼠的大鼠中检查了低聚乳蛋白牛奶对大鼠在牛奶出生的催乳激素成熟效应中的抗压力和孕产妇行为对脑功能发展的影响。溴克蛋白是一种催乳素分泌抑制剂，在妊娠的第20天和第21天被递送为腹膜内腔，在成年后，由溴克蛋白管理的母亲养育了耐压力和孕产妇行为。与对照母亲饲养的大鼠相比，由溴iocriptin-adminminminative母亲饲养的大鼠饲养的大鼠饲养的大鼠饲养幼崽的胃溃疡的程度显着增加。 These results suggest that milk-born prolactin plays an important role in establishment of neural networks for stress tolerance and maternal behavior.(2)Identification of a novel first exon of rat prolactin receptor gene and regulation of the prolactin receptor gene expression in the liver by sex steroid hormonesIn addition to the known four first exones, E1-1,E1-2,E1-3 and E1-4, another first exon, E1-5,was identified在大鼠催乳素受体基因中。肝脏中每个第一个外显子表达的表达分析表明，性类固醇激素特别调节E1-2第一外显子的表达（3）在大鼠大脑特异性外显子1，E1-4中，脑特异性受体基因表达的脑特异性调节机制E1-4，在每个Exon的表达中均表现为催乳素基因的表达和分析，在大脑基因2和3之间的表达中，在催乳素基因2和3之间进行了分析。发展取决于E1-4的表达。

项目成果

Growth hormone (GH)-stimulated insulin-like growth factor I gene expression is mediated by a tyrosine phosphorylation pathway depending on C-terminal region of human GH receptor in human GH receptor-expressing Ba/F3 cells.

• DOI: 10.1210/en.2003-0811
• 发表时间: 2004
• 期刊: Endocrinology
• 影响因子: 4.8
• 作者: H. Yoshizato;Minoru Tanaka;N. Nakai;N. Nakao;K. Nakashima

Comparison of ultrasonic vocalizations emitted by rodent pups

• DOI: 10.1538/expanim.53.409
• 发表时间: 2004-10-01
• 期刊: EXPERIMENTAL ANIMALS
• 影响因子: 2.4
• 作者: Hashimoto, H;Moritani, N;Saito, TR
• 通讯作者: Saito, TR

Effects of i.c.v. administration of leptin on copulatory and ingestive behavior in STZ-induced diabetic male rats.

静脉注射的影响

• 发表时间: 2004
• 期刊: Experimental Animals 53
• 作者: Hamasu T.;Inanami O.;Asanuma T;Kuwabara M.;Toru Saito
• 通讯作者: Toru Saito

Food intake and leptin concentrations of lactating rats nursing various sized litters

哺乳不同大小窝的哺乳大鼠的食物摄入量和瘦素浓度

• 发表时间: 2005
• 期刊: Reproduction Medical Biology 4(In press)
• 作者: 松村健;杉本千尋;Toru R.Saito
• 通讯作者: Toru R.Saito

Hideo Yoshizato: "Growth hormone (GH)-stimulated insulin-like growth factor I gene expression is mediated by a tyrosine phosphorylation pathway depending on C-terminal region of human GH receptor in human GH receptor-expressing Ba/F3 cells."Endocrinology.

Hideo Yoshizato：“生长激素 (GH) 刺激的胰岛素样生长因子 I 基因表达是由酪氨酸磷酸化途径介导的，该途径依赖于表达人 GH 受体的 Ba/F3 细胞中人 GH 受体的 C 末端区域。”内分泌学。