Basic research for antitumor immune response induced by dendritic cells pulsed with lysate of HSV-1-infected tumor cells

HSV-1感染肿瘤细胞裂解物脉冲树突状细胞诱导抗肿瘤免疫反应的基础研究

• 批准号: 17591501
• 负责人: TANAKA Minoru
• 金额: $ 1.98万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591501/
• 关键词: dendritic cell; HSV-1; Neuro 2a; A; J mouse; antitumor immune response; MHC class I; MHC class II; Oncolytic virus; dendritic cell; HSV-1; Neuro 2a; J mouse; antitumor immune response; MHC class I; MHC class II; Oncolytic virus; ウイルス; 免疫

In this study, we used oncolytic herpes simplex viruses as a new treatment for malignant brain tumors, researching methods to activate a tumor antigen more effectively than only using dendritic cells pulsed with a tumor antigen in tumor lysate.For anti-tumor effect, it is important to get co-stimulation acceptor stimulation as well as presenting tumor antigens to MHC I molecule. In order for T-lymphocytes to recognize the tumor antigen, MHC class II protein must also accompany the processed tumor antigen. We made subcutaneous tumor model of Neuro 2a and N18, neuroblastoma cell line of A/J mice. We used bone marrow-derived dendritic cells from A/J mice in order to confirm induction of antitumor immune response. As a result, antitumor immune response did not induced by pulsing these denciritic cells with lysate of T-01(HSV-1)-infected Neuro 2a tumor cells. However, we succeeded in induction of antitumor immune response by dendritic cells pulsed with N18 tumor cells.

在这项研究中，我们使用溶瘤性单纯疱疹病毒作为恶性脑肿瘤的一种新的治疗方法，研究了激活肿瘤抗原比仅使用肿瘤抗原在肿瘤裂解液中脉冲的树突状细胞更有效的方法。为了达到抗肿瘤的效果，重要的是获得共刺激受体的刺激以及将肿瘤抗原呈递到MHC I分子。为了使t淋巴细胞识别肿瘤抗原，MHC II类蛋白也必须伴随加工过的肿瘤抗原。我们制作了A/J小鼠神经母细胞瘤细胞系Neuro 2a和N18皮下肿瘤模型。我们使用来自A/J小鼠的骨髓来源的树突状细胞来证实抗肿瘤免疫反应的诱导。因此，用T-01（HSV-1）感染的neur2a肿瘤细胞的裂解液刺激这些树突状细胞并不能诱导抗肿瘤免疫反应。然而，我们成功地通过N18肿瘤细胞脉冲树突状细胞诱导抗肿瘤免疫反应。

项目成果

Functional neuronavigation co-utilizing tractography and white matter mapping (in Japanese)

联合使用纤维束成像和白质绘图的功能神经导航（日语）

• 发表时间: 2005
• 期刊: Currently Practical Neurosurgery 15(8)
• 作者: Kamada K;Todo T;Kawahara N;Kawai K;Morita A;Kirino T
• 通讯作者: Kirino T

Oncolytic virus therapy for glioma.

胶质瘤的溶瘤病毒治疗。

• 发表时间: 2005
• 期刊: Glioma-Clinical Pathology and Therapy (Tabuchi K (ed.)), Tokyo, Springer-Verlag
• 作者: Todo T;Ino Y
• 通讯作者: Ino Y

Dissociated expressive and receptive language functions on magnetoencephalography, functional magnetic resonance imaging, and amobarbital studies - Case report and review of the literature

• DOI: 10.3171/jns.2006.104.4.598
• 发表时间: 2006-04-01
• 期刊: JOURNAL OF NEUROSURGERY
• 影响因子: 4.1
• 作者: Kamada, K;Takeuchi, F;Sawamura, Y
• 通讯作者: Sawamura, Y

Triple gene-deleted oncolytic herpes simplex virus vector double -armed with IL-18 and soluble B7-1 constructed by bacterial artificial chromosome -mediated system.

细菌人工染色体介导系统构建的双臂IL-18和可溶性B7-1三基因缺失溶瘤单纯疱疹病毒载体。

• 发表时间: 2005
• 期刊: Cancer Res 65(23)
• 作者: Fukuhara H;Ino Y;Kuroda T;Martuza RL;Todo T
• 通讯作者: Todo T

Oncolytic virus therapy using genetically engineered herpes simplex viruses

• DOI: 10.1111/j.1749-0774.2002.tb00109.x
• 发表时间: 2002-08
• 期刊: Human Cell
• 影响因子: 4.3
• 作者: T. Todo