Oncogene and apoptosis related gene expression and radiation therapy respones of cervical cancer

为探讨肿瘤生长分数、细胞周期因子、癌基因、凋亡相关基因表达及组织学特征等生物学参数与放射治疗反应的关系，对64例宫颈癌患者进行了放射治疗后免疫组化检测，并以c-erbB-2癌基因表达作为预后分析指标结果：Ki-67免疫组化法测定肿瘤细胞生长分数（Ki-GF）、增殖细胞群有丝分裂指数（pMI），Ki-GF ≥ 33%者5年生存率明显高于Ki-GF <33%者（87.5%vs44.7%，P<0.01），差异主要是由于复发率的不同。pMI ≥ 3.5%的患者5年生存率明显低于pMI <3.5%的患者（0%vs81.8%，P<0.001），这种差异也是由于复发率的差异。 关于我们 erbB-OE表达于癌细胞膜上，p53表达与生存期无明显相关性，CerbB-OE阳性率为42.4%，随分期的进展而显著增加，Ki-GF和pMI分别为36%和2.5%，CerbB（+）患者Ki-GF平均为26.2%，显著低于CerbB（-）患者的38.3% CerbB（+）组pMI平均为3.70%，显著高于CerbB（-）患者的2.00% CerbB-OE与Ki-GF、pM 1的相关性表明，CerbB癌蛋白可能在宫颈癌细胞增殖状态中起重要作用。CerbB（+）和CerbB（-）患者的5年生存率分别为45.1%和75.6%，表明CerbB（+）患者的生存率明显低于CerbB（-）患者生存率的差异主要是由于局部复发而不是远处转移，Ki-GF，pMI和CerbB-OE可作为局部控制和长期生存的重要预测因子，有望作为选择最佳治疗方式的决定因素。少

Correlations between radiation response and varios biological parameters including growth fraction, cell cycle factors, oncogene, apoptosis relating gene expression of tumors and histologic features were investigated for prediction of local control probability following radiation therapy.Immunohistochemical study was performed on 64 cervical cancer patients treated with radiation therapy.Prognosis was analyzed by c-erbB-2 oncoprotein expression (CerbB-OE), p53 protein expression, growth fraction detemined with Ki-67 immunohistochemistry (Ki-GF) and the mitotic index of proliferating cell population (pMI).The 5-year survival rates for the Ki-GF of 33% or greater were significantly better than those below 33% (87.5% vs 44.7%, P<0.01).The difference was due to difference in recurrence rates. The 5-year survival rates for patients with pMI of 3.5% or greater were significantly lower than those below 3.5% (0% vs 81.8%, P<0.001).The difference was due also to difference in recurrence rates.C … More erbB-OE was observed on the cell membrane of cancer cells.There was no significant correlation between p53 expression and survival.Positivity of CerbB-OE,which was 42.4% in total, increased significantly with stage progression.Mean Ki-GF and pMI were 36% and 2.5% in total, respectively.Mean Ki-GF for CerbB(+) Patients was 26.2%, significantly lower than the 38.3% for CerbB (-) patients (P<0.01).The mean pMI for CerbB (+) patients was 3.70%, significantly higher than the 2.00% of CerbB (-) patients (P<0.05).The correlation between CerbB-OE and Ki-GF and pM1 suggests that CerbB oncoprotein may play an important role in cell proliferation status of cancer of the uterine cervix. The 5-year survival rates of CerbB (+) and CerbB (-) patients were 45.1% and 75.6%, respectively, indicating that CerbB (+) patients showed significantly poorer survival than CerbB (-) ones (P<0.01).The difference in survival was mainly due to local recurrence rather than distant metastasis.The Ki-GF,pMI and CerbB-OE are suggested as significant predictor to local control as well as long term survival.Theses predictors are hopeful as determinants for selecting optimal therapeutic modalities. Less