AUDITORY CORTICAL FUNCTIONS STUDIED BY IN-VIVO OPTICAL RECORDING METHOD

体内光学记录方法研究听觉皮层功能

• 批准号: 06680784
• 负责人: HORIKAWA Junsei
• 金额: $ 0.38万
• 依托单位: TOKYO MEDICAL AND DENTAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06680784/
• 关键词: Optical recording; Auditory cortex; Sound orientation; FM sounds; Animal calls; Guinea pigs; 両耳性神経活動; 時空間パターン

Using an optical recording method with a 12 * 12-channel photodiode array and a voltage-sensitive dye (RH795), spatiotemporal patterns of neural activities in anterior (A : primary) and dorsocaudal (DC) fields of the guinea pig auditory cortex were measured under anesthetized condition (Nembutal 30 mg/kg). In this research, two projects were conducted : (1) binaural interaction and (2) information processing of frequency-modulated (FM) sounds and animal calls.To measure binaural interaction, we used both free-field stimulation and closed-ear stimulation. The optically-recorded responses to free-field stimulation showed that the responses were maximal when the speaker was placed at around 30 degrees contralateral to the recoding side and small when it was placed on the ipsilateral side. The azimuth selectivity was sharper for higher frequencies and broader for lower frequencies. These response patterns were suggested to be a reflection of pinna characteristics. Cortical locations specif … More ically responding to a certain azimuth were not observed, indicating that there is no topographic representation of azimuth in fields A and DC.Closed-ear stimulation revealed that the excitation was evoked mainly contralaterally and the inhibition was evoked mainly ipsilaterally. This excitation and inhibition occurred at the same cortical places in both fields.The responses to FM sounds and animal calls were different from those to pure-tones. The response to a pure-tone moved dorsally along an isofrequency band, but those to FM sounds moved obliquenly crossing different isofrequency bands. The direction of the response movement was opposite between upward and downward frequency sweeps. The responses to animal calls were very complicated : they showed several times repetition of excitation and inhibition during the calls. No cortical place which specifically responds to FM sounds and the calls was found. These results indicate that spatiotemporal patterns in response to the FM sounds and the calls are related to the temporal structures of spectral components of these sounds. Less

使用12 * 12通道光电二极管阵列和电压敏感染料（RH795）的光学记录方法，在麻醉条件下（戊巴比妥30 mg/kg）测量豚鼠听觉皮层前区（A：初级）和背尾区（DC）神经活动的时空模式。在这项研究中，进行了两个项目：（1）双耳交互和（2）调频（FM）声音和动物叫声的信息处理。为了测量双耳交互，我们使用了自由场刺激和闭耳刺激。对自由场刺激的光学记录反应表明，当扬声器放置在与记录侧对侧约 30 度角时，反应最大；而当扬声器放置在同侧时，反应较小。频率越高，方位选择性越强，频率越低，方位选择性越宽。这些反应模式被认为反映了耳廓特征。没有观察到皮质位置对特定方位角的特异性响应，这表明在 A 区和 DC 区没有方位角的地形表征。闭耳刺激显示，兴奋主要在对侧引起，抑制主要在同侧引起。这种兴奋和抑制发生在两个区域的相同皮质位置。对调频声音和动物叫声的反应与对纯音的反应不同。对纯音的响应沿着等频带向背侧移动，但对 FM 声音的响应则倾斜地穿过不同的等频带。向上和向下频率扫描之间的响应运动方向相反。对动物叫声的反应非常复杂：它们在叫声过程中表现出多次重复的兴奋和抑制。没有发现专门对 FM 声音和呼叫做出反应的皮质区域。这些结果表明，响应 FM 声音和呼叫的时空模式与这些声音的频谱分量的时间结构有关。较少的

项目成果

Horikawa,J.et al.: "Effects of inhibitory and excitatory channel blockers on neural responses of guinea pig auditory cortex studied by in-vivo optical recording." Jpn.J.Physiol.44. S195 (1994)

Horikawa, J. 等人：“通过体内光学记录研究抑制性和兴奋性通道阻滞剂对豚鼠听觉皮层神经反应的影响。”

Hosokawa, Y., J.Horikawa, M.Nasu and I.Taniguchi: "Binaural interaction in the guinea pig auditory cortex observed by optical recording." Assoc.Res.Otolaryngol.Abstr. 23-93 (1994)

Hosokawa, Y.、J.Horikawa、M.Nasu 和 I.Taniguchi：“通过光学记录观察豚鼠听觉皮层的双耳相互作用。”

Horikawa, J., I.Taniguchi, Y.Hosokawa, M.Kubota and M.Nasu: "Effects of inhibitory and excitatory channel blockers on neural responses of guinea pig auditory cortex studied by in-vivo optical recording." Jpn.J.Physiol. 44, Suppl.1. S195 (1994)

Horikawa, J.、I.Taniguchi、Y.Hosokawa、M.Kubota 和 M.Nasu：“通过体内光学记录研究抑制性和兴奋性通道阻滞剂对豚鼠听觉皮层神经反应的影响。”

森山俊男,他: "異なった方向からの音刺激によるモルモット皮質聴覚領の両耳応答の時空間パターン" Audiol.Jpn.38. 735-736 (1995)

Toshio Moriyama等人：“豚鼠听觉区域对不同方向声音刺激的双耳反应的时空模式”Audiol.Jpn.38（1995）。

Horikawa, J. et al.: "Optical imaging of spatiotemporal patterns of glutamatergic excitartion and GABAergic inhibition in the guinea pig auditory cortex in vivo." J. Physiol.(in press). (1996)

Horikawa, J. 等人：“豚鼠听觉皮层体内谷氨酸能兴奋和 GABA 能抑制的时空模式的光学成像。”