High-performance Stent Technology

高性能支架技术

• 批准号: 15200038
• 负责人: MATSUDA Takehisa
• 金额: $ 30.28万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15200038/
• 关键词: stent; scaffold; endothelial cell; 内皮前駆細胞; 光化学

This study aimed at developing high biocompatible stent technology based on ex vivo cell seeding by combination of cell engineering, matrix engineering and scaffold engineering, by which rapid complete endothelialization on an implanted stent as to fully prevent thrombus formation, thus avoiding stenosis and occlusion, will be achieved. To this end, the authors developed endothelial cell and endothelial progenitor cell, both of which are harvested from autologous tissue or blood, are seeded and cultured to form monolayered tissue on the whole body of stent. As for matrix engineering, vinylated biomolecules include albumin, gelatin and heparin were photopolymerized under visible light-irradiation to form completely cover the stent surface prior to cell seeding. As for scaffold engineering, the authors devised electrospinning method using synthetic biodegradable elastomer (copolymer of lactide and caprolactone) to form a mesh-type cover stent. Bioactive substances such as collagen, heparin and growth factor were also co-electronspun. It is envisaged that the combination of these technologies expectantly enhances the thrombus-free condition which starts immediately after implantation.

本研究旨在开发基于细胞工程、基质工程和支架工程相结合的体外细胞接种的高生物相容性支架技术，通过该技术，将实现植入支架上的快速完全内皮化，从而完全防止血栓形成，从而避免狭窄和闭塞。为此，作者开发了内皮细胞和内皮祖细胞，两者均从自体组织或血液中收获，接种并培养以在支架的整个主体上形成单层组织。对于基质工程，在可见光照射下使乙烯基化的生物分子（包括白蛋白、明胶和肝素）光聚合以在细胞接种之前形成完全覆盖支架表面。在支架工程方面，作者设计了使用合成的可生物降解弹性体（丙交酯和己内酯的共聚物）的静电纺丝方法来形成网状覆盖支架。生物活性物质，如胶原蛋白，肝素和生长因子也共同电子自旋。据设想，这些技术的组合预期会增强植入后立即开始的无血栓状态。

项目成果

Mechano-active scaffold design of small-diameter artificial graft made of electrospun segmented polyurethane fabrics

• DOI: 10.1002/jbm.a.30260
• 发表时间: 2005-04-01
• 期刊: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
• 影响因子: 4.9
• 作者: Matsuda, T;Ihara, M;Kidoaki, S
• 通讯作者: Kidoaki, S

Poly(N-isopropylacrylamide)(PNIPAM)-graft gelatin hydrogel surface : interrelationship between microscopic structure and mechanical property of surface regions and cell adhesiveness.

聚（N-异丙基丙烯酰胺）（PNIPAM）接枝明胶水凝胶表面：表面区域的微观结构和机械性能以及细胞粘附性之间的相互关系。

• 发表时间: 2005
• 期刊: Biomaterials 26
• 作者: S.Ohya;S.Kidoaki;T.Matsuda
• 通讯作者: T.Matsuda

Matsuda T, Nagase J, Ghoda A, Hirano Y, Kidoaki S, Nakayama Y.: "Phosphorylcholine-endcapped oligomer and block co-oligomer and surface biological reactivity"Biomaterials. 24. 4517-4527 (2003)

Matsuda T、Nagase J、Ghoda A、Hirano Y、Kidoaki S、Nakayama Y.：“磷酸胆碱封端低聚物和嵌段共聚低聚物和表面生物反应性”生物材料。

Shirota T, He H, Yasui H, Matsuda T.: "Human endothelial progenitor cell(EPC)-seeded hybrid graftproliferative and antithrombogenic potentials of EPC"Tissue Eng. 9(1). 127-136 (2003)

Shirota T、He H、Yasui H、Matsuda T.：“人内皮祖细胞（EPC）接种的混合移植物的 EPC 增殖和抗血栓形成潜力”组织工程。

Mesoscopic spatial designs of nano- and micron-Fiber meshes for tissue-engineering matrix and scaffold based on newly devised multilayering and mixing electrospinning techniques.

基于新设计的多层和混合静电纺丝技术的组织工程基质和支架的纳米和微米纤维网格的介观空间设计。

• 发表时间: 2005
• 期刊: Biomaterials 26
• 作者: S.Kidoaki;I.K.Kwon;T.Matsuda
• 通讯作者: T.Matsuda