Mechanical Stress-Loading on Cellular Responses of Vascular Cell Types

机械应力加载对血管细胞类型细胞反应的影响

• 批准号: 07458241
• 负责人: MATSUDA Takehisa
• 金额: $ 4.74万
• 依托单位: Research Institution of National Cardiovascular Center
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07458241/
• 关键词: Mechanical Stress; Vascular Cells; Extracellular Matrix

This study was conducted to study the responses of cells exposed to mechanical stress fild, which include orientation and elongation of adhered cells on 2-dimensional substrate in vitro and morphogenesis and tissue formation of vascular wall in vivo under continuously loaded mechanical stress fields.Upon continuous cyclic distension of adhered cells, cells oriented parallel to the direction of distension with loading time of mechanical stress. On the other hand, hybrid vascular grafts prepared using vascular cell types (endothelial cell ; EC,smooth muscle cell ; SMC and fibroblast ; FC) underwent to remodel themselves upon implantation. ECs highly elongated to the direction of flow, whereas SMCs are circumferentially oriented. Extracellular matrix (ECM) polymers secreted by cells underwent self-assembling and organized to form fiber formation with time. These ECM polymers include collagen and elastin. The former constructed a network of fiber highly oriented circumfererfially, and th later formed a layred honey-comb structure. Thus, sellular and macromolecular orientation proceeded in time to restore vital structure and function of neoarterial vessels.

本研究旨在研究细胞在机械应力场中的响应，包括体外二维基质上贴壁细胞的取向和伸长，以及体内血管壁在连续加载机械应力场作用下的形态发生和组织形成。另一方面，使用血管细胞类型(内皮细胞、内皮细胞、平滑肌细胞、成纤维细胞和成纤维细胞；FC)制备的混合血管移植物在植入后进行自我重建。ECS沿流动方向高度伸长，而SMC沿圆周方向取向。细胞分泌的细胞外基质(ECM)聚合物随着时间的推移自组装并组织形成纤维。这些ECM聚合物包括胶原和弹性蛋白。前者在周向形成高度取向的纤维网络，后者形成层状蜂窝状结构。因此，小叶和大分子定位及时进行，以恢复新生动脉的重要结构和功能。

项目成果

N.Saito,T.Sugawara,T.Matsuda: "Synthesis and Hydrophilicity of Multifunctionally Hydroxylated Poly (acrylamides)" Macromolecules. 29. 313-319 (1996)

N.Saito、T.Sugarara、T.Matsuda：“多功能羟基化聚（丙烯酰胺）的合成和亲水性”大分子。

K.Doi, Y.Nakayama, T.Matsuda: "Novel compliant and Tissue-Permeable Microporous Polyurethane Vascular Prosthesis Fabricated Using and Excimer Laser Ablation Technique" Journal of Biomedical Materials Research. 31. 27-33 (1996)

K.Doi、Y.Nakayama、T.Matsuda：“使用准分子激光烧蚀技术制造的新型顺应性且组织可渗透的微孔聚氨酯血管假体”生物医学材料研究杂志。

松田武久: "血管壁再構築と分子細胞生物学" 日本ME学会 BME. 9. 44-55 (1995)

Takehisa Matsuda：“血管壁重建和分子细胞生物学”日本 ME 学会，BME 9. 44-55 (1995)。

T.Matsuda,T.Sugawara: "Control of cell adhasion,migration,and orientation on photochemically microprocessed surfaces" Journal of Biomedical Materials Research. 32. 165-173 (1996)

T.Matsuda、T.Sukawara：“光化学微加工表面上细胞粘附、迁移和方向的控制”生物医学材料研究杂志。

神田圭一: "新しい小口径人工血管:高密度・高配向コラーゲン管状組織体" 人工臓器. 24. 185-189 (1995)

Keiichi Kanda：“新型小直径人造血管：高密度和高度定向的胶原管状组织”Artificial Organs。 24. 185-189 (1995)。