Molecular basis of oil-degrading methanogenic microbial symbiotic system indigenous to petroleum reservoir

为获得石油储层内源性降解原油产甲烷微生物的工业应用基础知识，采集了石油储层卤水样品，分析了储层中微生物的种群结构和产甲烷活性。我们的研究结果表明，在生物降解油藏内的微生物系统中的乙酸裂解产甲烷菌的重要作用。此外，我们表明，补充复合营养物质可以提高微生物的产甲烷活性内源性枯竭油藏。

To gain basic knowledge for industrial application of crude-oil-degrading methanogenic microorganisms endogenous to petroleum reservoir, we collected reservoir brine samples from petroleum reservoirs and analyzed population structure and methanogenic activity of microorganisms in the reservoirs. Our results suggested an important role of acetoclastic methanogens in the microbial system within a biodegraded oil reservoir. Moreover, we showed that supplementation of complex nutrients could enhance the methanogenic activity of microorganisms endogenous to a depleted oil reservoir.