Fundamental Study on the Accumulation of Environmental hormone into the Biopolymer Conjugates

生物聚合物缀合物积累环境激素的基础研究

• 批准号: 11450359
• 负责人: NISHI Norio
• 金额: $ 6.78万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11450359/
• 关键词: DNA; Chitin-Chitosan; Collagen; Biopolymer Conjugate; Environmental Hormone; Intercalation; Dioxin; Benzopyrene; 生体高分子複合素材; DNA素材; シクロデキストリン素材; PCB; 水不溶性DNAフィルム; シクロデキストリン; ビスフェノールA; 濃縮・分離システム

In our laboratory, utilization of discarded biomaterials has been investigated. Especialy, chitin-chitosan from crab shell, DNA from salmon milt, collagen from calf skin and their conjugated materials as biofunctional materials have been studied exhaustively. In the process f these investigations, we found that insolubilized DNA film adsorbed DNA-binding intercalating reagents, such as ethidium bromide and benzopyrene. In the present study, we have investigated the interaction of DNA or DNA-conjugated materials and environmental hormons (endocrine disruptors). As a result, DNA or DNA-conjugated materials can accumulate the environmental hormons with planar structure, such as dioxin derivatives, polychlorobiphenyl (PCB) derivatives and benzopyrene. On the other hand, endocrine disruptors without planar structure, such as bisphenol A and diethylstilbestrol, were not accumulated at all. These results suggested that DNA and DNA-conjugated materials were able to remove harmful environmental hormones with the planar structure.

在我们的实验室，废弃生物材料的利用进行了研究。特别是对蟹壳甲壳素-壳聚糖、鲑鱼鱼白DNA、牛皮胶原蛋白及其复合材料作为生物功能材料进行了详尽的研究。在这些研究的过程中，我们发现不溶解的DNA膜吸附DNA结合嵌入试剂，如溴化乙锭和苯并芘。在本研究中，我们研究了DNA或DNA缀合材料与环境激素（内分泌干扰物）的相互作用。因此，DNA或与DNA结合的材料可以积累具有平面结构的环境有害物质，如二恶英衍生物、多氯联苯（PCB）衍生物和苯并芘。另一方面，没有平面结构的内分泌干扰物，例如双酚A和己烯雌酚，根本不会积累。这些结果表明，DNA和DNA缀合材料能够以平面结构去除有害的环境激素。

项目成果

Masanori Yamada: "UV-Irradiated DNA Matrixes Selectively Bind Endocrine Disruptors with a Planar Structure"ENVIRON.SCI.& TECHNOL..

Masanori Yamada：“紫外线照射的 DNA 基质通过平面结构选择性地结合内分泌干扰物”ENVIRON.SCI。

Isao Ohiso: "Conformational Study of the Tandem Repeat Sequence in RNA Polymerase II by Circular Dichroism Spectroscopy"Bull.Chem.Soc.Jpn.. 74. 1139-1143 (2001)

Isao Ohiso：“通过圆二色光谱法对 RNA 聚合酶 II 中串联重复序列的构象研究”Bull.Chem.Soc.Jpn.. 74. 1139-1143 (2001)

Masayoshi MAKINO: "Biologically Active Sequences in Lamininn, a Multifunctional Extracelluar Matrix Protein"Connective Tissue. 8 (1999)

Masayoshi MAKINO：“层粘连蛋白中的生物活性序列，一种多功能细胞外基质蛋白”结缔组织。

Masanori Yamada: "Preparation and Characterization of DNA Films Induced by UV Irradiation"Chem. Eur. J.. 8(6). 1407-1412 (2002)

Masanori Yamada：“紫外线照射诱导的 DNA 薄膜的制备和表征”Chem。

Akiko Iihara: "Effect of Nucloeplasmin on Stability of Nucleosome Structure"Peptide Sci.1999. 363-366 (2000)

Akiko Iihara：“核纤蛋白对核小体结构稳定性的影响”肽科学，1999。