Fundamental Study on the Accumulation of Environmental hormone into the Biopolymer Conjugates

在我们的实验室，废弃生物材料的利用进行了研究。特别是对蟹壳甲壳素-壳聚糖、鲑鱼鱼白DNA、牛皮胶原蛋白及其复合材料作为生物功能材料进行了详尽的研究。在这些研究的过程中，我们发现不溶解的DNA膜吸附DNA结合嵌入试剂，如溴化乙锭和苯并芘。在本研究中，我们已经调查了DNA或DNA结合的材料和环境中的内分泌干扰物（内分泌干扰物）的相互作用。因此，DNA或与DNA结合的材料可以积累具有平面结构的环境有害物质，如二恶英衍生物、多氯联苯（PCB）衍生物和苯并芘。另一方面，没有平面结构的内分泌干扰物，如双酚A和己烯雌酚，根本没有积累。这些结果表明，DNA和DNA结合材料能够去除有害的环境激素的平面结构。

In our laboratory, utilization of discarded biomaterials has been investigated. Especialy, chitin-chitosan from crab shell, DNA from salmon milt, collagen from calf skin and their conjugated materials as biofunctional materials have been studied exhaustively. In the process f these investigations, we found that insolubilized DNA film adsorbed DNA-binding intercalating reagents, such as ethidium bromide and benzopyrene. In the present study, we have investigated the interaction of DNA or DNA-conjugated materials and environmental hormons (endocrine disruptors). As a result, DNA or DNA-conjugated materials can accumulate the environmental hormons with planar structure, such as dioxin derivatives, polychlorobiphenyl (PCB) derivatives and benzopyrene. On the other hand, endocrine disruptors without planar structure, such as bisphenol A and diethylstilbestrol, were not accumulated at all. These results suggested that DNA and DNA-conjugated materials were able to remove harmful environmental hormones with the planar structure.