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Utilization of DNA as functional materials

DNA作为功能材料的利用

基本信息

批准号：
07555600
负责人：
NISHI Norio
金额：
$ 1.92万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07555600/
关键词：
DNA functional material DNA-alginic acid ELISA method anti-DNA antibody antibacterial activity silver ion antibacterial film 細胞増殖抑制発癌性物質魚類白子 DNA-アルギン酸複合体ベンツピレン

项目摘要

DNA,one of the most important materials in life science, can be regarded as naturally occurring and highly specific functional biopolymer. In the present project, utilization of DNA as functional materials was intended.Thin films composed of DNA and alginic acid were prepared by casting their mixed solution on glass plate followed by coagulation with aqueous solution of calcium chloride. DNA could be conveniently insolubilized by this method. DNA in the films absorbed intercalating materials, such as ethidium bromide. This phenomenon was successfully applied to the preparation of filters for the selective removal or accumulation of harmful intercalating pollutants.DNA was effectively complexed and immobilized in an alginate gel and anti-DNA antibodies bound to the DNA-alginate complex. Dose-dependent binding of anti-DNA antibody was observed on the polystirene plates coated with the complex. These results indicate that the DNA-alginate complex may be useful as a solid phase antigen for detection of anti DNA antibodies in enzyme-linked immunosorbent assay (ELISA).Silver ion was effectively bound to alginate film by the aid of DNA.The Ag (I)-induced films showed antibacterial activity for both of E.coli and S.aureus, and the activity of DNA-alginate film. These results indicate that Ag (I) induced in the DNA-alginate film keeps the potent antibacterial activity, and so DNA is very useful as a carrier of silver ion in the alginate film.

DNA是一种天然存在的具有高度特异性功能的生物高聚物，是生命科学中最重要的材料之一。本项目拟利用DNA作为功能材料。将DNA与海藻酸的混合溶液浇铸在玻璃板上，与氯化钙水溶液混凝，制备了海藻酸与DNA的薄膜。这种方法可以方便地将DNA溶解。薄膜中的DNA吸收了插入物，如溴化乙锭。这一现象成功地应用于过滤器的制备，以选择性地去除或积累有害的插层污染物。DNA在海藻酸盐凝胶中有效地络合和固定，抗DNA抗体结合到DNA-海藻酸盐复合物上。在包被该复合物的聚苯乙烯板上观察到抗dna抗体的剂量依赖性结合。这些结果表明，DNA-海藻酸盐复合物可作为酶联免疫吸附试验（ELISA）中检测抗DNA抗体的固相抗原。银离子在DNA的作用下与海藻酸盐膜有效结合。Ag (I)诱导膜对大肠杆菌和金黄色葡萄球菌均有抑菌活性，对dna -海藻酸盐膜也有抑菌活性。这些结果表明，银离子在DNA-藻酸盐膜中的诱导作用保持了较强的抗菌活性，因此DNA在藻酸盐膜中作为银离子的载体是非常有用的。