Joint Research on Preparation of Novel Bioconjugates

新型生物结合物制备联合研究

• 批准号: 11694114
• 负责人: NISHI Norio
• 金额: $ 5.7万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11694114/
• 关键词: Bioconjugate; protein; nucleic acid; polysaccharide; bio-related polymer; DNA-chitosan bilayer membrane; DNA-collagen bioconjugate; Insolubilized DNA; ペプチド; PNA-コラーゲン複合体; 糖ペプチド; DNA-キトサン複合体; 糖タンパク質

Research on bio-related polymeric materials, such as nucleic acid(DNA and RNA), peptide, protein, polysaccharide, lipid, has developed extensively in resent years. However, the each research fields for these materials have their own world, and their mutual exchange and colaborations are not popular so far. Very fine functional materials would be found between their field or in their conjugated fields. We intended to prepare novel bioconjugate by the contact discussion and colaboration of Japanese and German researchers on various biorelated polymers.As a result, following researches have been carried out.1. Preparation of DNA-chitosan bilayer membrane for surgecal use.2. Basic research on syntehsis of glycopeptide and glycoprotein.3. Insolubilization of DNA by UV-irradiation.4. Preparation of DNA-collagen bioconjugate.

近年来，核酸（DNA和RNA）、多肽、蛋白质、多糖、脂质等生物相关高分子材料的研究得到了广泛的发展。然而，这些材料的研究领域各不相同，相互间的交流与合作还不多见。在它们的场之间或它们的共轭场中会发现非常精细的功能材料。本课题旨在通过与日本和德国的研究人员就各种生物相关聚合物的接触讨论和合作，制备新型生物偶联物，为此，开展了以下研究工作.医用DNA-壳聚糖双层膜的制备.糖肽和糖蛋白合成的基础研究.紫外线照射对DNA的不溶解作用。DNA-胶原生物缀合物的制备。

项目成果

Masanori Yamada, Kozue Kato, Kazuna Shindo, Motoyoshi Nomizu, Nobuo Sakairi, Hiroyuki Yamamoto, Norio Nishi: "Immobilization of DNA by UV irradiation and its utilization as functional materials"Nucleic Acids (Sympo. Series). 42. 103-104 (1999)

Masanori Yamada、Kozue Kato、Kazuna Shindo、Motoyoshi Nomizu、Nobuo Sakairi、Hiroyuki Yamamoto、Norio Nishi：“通过紫外线照射固定DNA及其作为功能材料的利用”核酸（Sympo.系列）。

Akiko Iihara: "Effect of Nucloeplasmin on Stability of Nucleosome Structure"Peptide Sci.1999. 363-366 (2000)

Akiko Iihara：“核纤蛋白对核小体结构稳定性的影响”肽科学，1999。

西 則雄: "サケ白子DNAの機能性素材への利用"機能材料. 8 (1999)

Norio Nishi：“将鲑鱼鱼白 DNA 用于功能材料”功能材料 8 (1999)。

Isao Ohiso: "Theoretical Conformational Analysis of the Tandem Repeat Sequence in RNA Polymerase II"Bull.Chem.Soc.Jpn.. 74. 1847-1855 (2001)

Isao Ohiso：“RNA 聚合酶 II 中串联重复序列的理论构象分析”Bull.Chem.Soc.Jpn.. 74. 1847-1855 (2001)

西則雄: "ダイオキシンを捕まえるDNA"高分子学会出版部編(印刷中).

西纪夫：《捕获二恶英的DNA》，高分子科学学会出版部编（正在出版）。