Chemical Synthesis of Highly Functional Artificial Protein.

高功能人工蛋白质的化学合成。

• 批准号: 63550675
• 负责人: NISHI Norio
• 金额: $ 1.28万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63550675/
• 关键词: Highly functional artificial protein; Protein-nucleic acid interaction; Protamine; DNA-binding protein; DNA-aggregating mechanism; Protamine-model peptide; RNA polymerase; Inhibition in the metastases of malignant melanoma; 細胞接着性蛋白質; 蛋白質一核酸相互作用 /

Protein-nucleic acid interaction is one of the very important ones among the various interactions in life science. From this standpoint, DNA-binding proteins, such as protamine in fish sperm, were chosen as the main target proteins of the present project, "Chemical synthesis of highly functional artificial protein". As approaches to the protamine function, (1)protamine-model peptides, (2)protamine dimer and trimer, (3)Pro-replaced protamine analogue were chemically synthesized. Investigations about their protamine-like functions suggested one special DNA-binding mechanism of protamine, in which its steric conformation, turn structure for example, participated.A project for the second target DNA-binding protein, RNA polymerase II, also started. In addition, sequential polypeptides which have inhibitory effect on the metastases of malignant melanoma were successfully synthesized as examples of highly functional artificial protein.

蛋白质-核酸相互作用是生命科学中各种相互作用中非常重要的一种。从这一观点出发，选择了DNA结合蛋白质，如鱼精蛋白，作为本项目“高功能人工蛋白质的化学合成”的主要目标蛋白质。作为研究鱼精蛋白功能的方法，我们采用化学方法合成了鱼精蛋白模型肽，鱼精蛋白二聚体和三聚体，鱼精蛋白脯氨酸取代的类似物。对鱼精蛋白类功能的研究表明，鱼精蛋白的空间构象参与了与DNA结合的特殊机制，而第二个靶点RNA聚合酶II的研究也开始了。此外，作为高功能人工蛋白的实例，成功地合成了对恶性黑色素瘤转移具有抑制作用的序列多肽。

项目成果

Norio Nishi: "Simulation of Protamine Function by Peptide Chemistry" Peptide Chem.1989. (1990)

Norio Nishi：“通过肽化学模拟鱼精蛋白功能”Peptide Chem.1989。

Norio Nishi: "Study on the DNA Aggregation Induced by Protamine" Rept. Progr.Polym. Phys. Jpn., 31, 581-584(1988).

Norio Nishi：“鱼精蛋白诱导的 DNA 聚集的研究”Rept.

Norio Nishi: "Study on the DNA Aggregation Induced by Protamine" Rept.Progr.Polym.Phys.Jpn.31. 581-584 (1988)

Norio Nishi：“鱼精蛋白诱导的 DNA 聚集的研究”Rept.Progr.Polym.Phys.Jpn.31。

Ikuo Saiki: "Inhibition of the Metastases of Murine Malignant Melanoma by Synthetic Polymeric Peptides Containing Core Sequences of Cell Adhesive Molecules." Cancer Res., 49, 3815-3822(1989).

Ikuo Saiki：“含有细胞粘附分子核心序列的合成聚合肽抑制小鼠恶性黑色素瘤的转移。”

Ikuo Saiki: "Inhibition of the Metastases of Murine Malignant Melanoma by Synthetic Polymeric Peptides Containing Core Sequences of Cell Adhesive Molecules." Cancer Res.49. 3815-3822 (1989)

Ikuo Saiki：“含有细胞粘附分子核心序列的合成聚合肽抑制小鼠恶性黑色素瘤的转移。”