Chemical Synthesis of Highly Functional Artificial Protein.

蛋白质-核酸相互作用是生命科学中各种相互作用中非常重要的一种。从这个角度来看，选择dna结合蛋白，如鱼精子中的鱼精蛋白，作为本项目“化学合成高功能人工蛋白”的主要靶蛋白。作为研究鱼精蛋白功能的途径，(1)鱼精蛋白模型肽，(2)鱼精蛋白二聚体和三聚体，(3)原替代鱼精蛋白类似物的化学合成。对它们类鱼精蛋白功能的研究表明，鱼精蛋白具有一种特殊的dna结合机制，其立体构象，如旋向结构等参与了这种机制。第二个目标dna结合蛋白RNA聚合酶II的项目也开始了。此外，还成功合成了抑制恶性黑色素瘤转移的序列多肽，作为高功能人工蛋白的例子。

Protein-nucleic acid interaction is one of the very important ones among the various interactions in life science. From this standpoint, DNA-binding proteins, such as protamine in fish sperm, were chosen as the main target proteins of the present project, "Chemical synthesis of highly functional artificial protein". As approaches to the protamine function, (1)protamine-model peptides, (2)protamine dimer and trimer, (3)Pro-replaced protamine analogue were chemically synthesized. Investigations about their protamine-like functions suggested one special DNA-binding mechanism of protamine, in which its steric conformation, turn structure for example, participated.A project for the second target DNA-binding protein, RNA polymerase II, also started. In addition, sequential polypeptides which have inhibitory effect on the metastases of malignant melanoma were successfully synthesized as examples of highly functional artificial protein.