Regenerative Medicine for the Type 2 Diabetes

2 型糖尿病的再生医学

• 批准号: 15390283
• 负责人: KOJIMA Itaru
• 金额: $ 9.6万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15390283/
• 关键词: insulin; diabetes; regenerative medicine; stem cell; cell transplantation; cell therapy; 膵β細胞; 再生医学; 分化誘導因子

1)We isolated pancreatic ductal cells obtained from neonatal rats and cultured in vitro. We added two differentiation factors, activin A and betacellulin, and converted them to insulin-producing cells. Using differentiated cells, we prepared pseudoislets. The insulin content and insulin secretion were much improved after the formation of pseudoislets and, in particular, insulin secretory response to glucose was markedly improved. We then transplanted these pseudoislets into the portal vein of the streptozotocin-treated diabetic nude mice. The plasma glucose concentration was improved by transplantation of the pseudoislets.2)For regenerative therapy, stem cells should be expanded easily. We are interested in hepatic stem-like cells, which can be expanded very easily in vitro. These cells express α-fetoprotein and CK19 and convert to both hepatocytes and duct epithelial cells. We converted these cells to cells expressing insulin and PGP9.5.

1)分离新生大鼠胰腺导管细胞，进行体外培养。我们加入了激活素A和β细胞蛋白两种分化因子，并将它们转化为胰岛素产生细胞。利用分化的细胞，我们制备了假性胰岛。假性胰岛形成后，胰岛素含量和胰岛素分泌明显改善，尤其是胰岛素对葡萄糖的分泌反应明显增强。然后，我们将这些假性胰岛移植到链脲佐菌素治疗的糖尿病裸鼠的门静脉中。移植伪岛可以改善血糖浓度。2)对于再生治疗，干细胞应易于扩增。我们对肝干细胞样细胞很感兴趣，它可以很容易地在体外扩增。这些细胞表达α胎蛋白和CK19，并向肝细胞和导管上皮细胞转化。我们将这些细胞转化为表达胰岛素和PGP9.5的细胞。

项目成果

Li L, Seno M, Yamada H, Kojima I: "Betacellulin improves glucose metabolism by promoting conversion of intraislet precursor cells in STZ-treated mice."Am J Physiol. 258. E577-E583 (2003)

Li L、Seno M、Yamada H、Kojima I：“Betacellulin 通过促进 STZ 处理的小鼠胰岛内前体细胞的转化来改善葡萄糖代谢。”Am J Physiol。

Li L, Zhaohog Y, Seno M, Kojima I: "Activir A and betacellulin i effect on regeneration of panirectic β cells in neonatal STZ-treated rats."Diabetes. 53. 608-615 (2004)

Li L、Zhaohog Y、Seno M、Kojima I：“Activir A 和 betacellulin i 对新生 STZ 治疗大鼠的全皮β细胞再生的影响。”糖尿病。53. 608-615 (2004)

Activin A and betacellulin : effect on regeneration of pancreatic β cells in neonatal STZ-treated rats.

激活素 A 和 betacellulin：对新生 STZ 治疗大鼠胰腺 β 细胞再生的影响。

• 发表时间: 2004
• 期刊: Diabetes 53 March
• 作者: Li;L
• 通讯作者: L

Promotion of (β-cell differentiation by conophylline in fetal and neonatal rat pancreas.

康茶碱促进胎儿和新生大鼠胰腺中β细胞分化。

• 发表时间: 2004
• 期刊: Diabetes 53 October
• 作者: Ogata;T
• 通讯作者: T

Reversal of streptozotocin induced-diabetes hyperglycemia by transplantation of pseudoislets consisting of β cells derived from ductal cells.

通过移植由导管细胞衍生的 β 细胞组成的假胰岛来逆转链脲佐菌素诱导的糖尿病高血糖。

• 发表时间: 2004
• 期刊: Endocrine Journal 51(3)
• 作者: Ogata;T
• 通讯作者: T