Antinucleic aid antibody gene therapy for rheumatoid Arthritis and adult T cell Leukemia

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1.Expression of antibody against DNARNA hybrid in the cells.We first obtained hybridoma producing monoclonal anti-DMA RNA hybrid antibody and prepared the fragments of VH and VL gene encoding the antibody. Both fragments were bound for the expression of ScFv antibodies and introduced into vectors for the expression in the cells.2. Efficacy of gene therapy using anti-ScFv expression system for prevention of tabacco mosaic virus (TMV) infection.Tabacco which was introduced with anti-DNARNA hybrid ScFv genes, expressed ScFv mRNA and showed marked resistance to TMV infection.3. Blocking experiment of human adult T cell leukemia virus (HTLV-1) infection by gene therapy using anti-ScFv expression system.We are establishing for evaluation of efficacy of ScFv strategy in order to block HTLV-1 infection in T cells.