Antinucleic aid antibody gene therapy for rheumatoid Arthritis and adult T cell Leukemia

1.在细胞中表达抗DNARNA杂交瘤抗体我们首次获得了产生抗DNARNA杂交瘤抗体的单克隆杂交瘤细胞，并制备了编码该抗体的VH和VL基因片段。将两个片段结合用于表达单链抗体，并将其导入载体用于在细胞中表达.抗ScFv基因表达系统对烟草花叶病毒（TMV）感染的基因治疗效果研究将抗DNARNA杂合ScFv基因导入烟草，获得了ScFv mRNA表达，并对TMV感染表现出明显的抗性.利用抗单链抗体表达系统进行人成人T细胞白血病病毒（HTLV-1）感染的基因治疗阻断实验，旨在建立评价抗单链抗体策略阻断HTLV-1感染T细胞的有效性。

1.Expression of antibody against DNARNA hybrid in the cells.We first obtained hybridoma producing monoclonal anti-DMA RNA hybrid antibody and prepared the fragments of VH and VL gene encoding the antibody. Both fragments were bound for the expression of ScFv antibodies and introduced into vectors for the expression in the cells.2. Efficacy of gene therapy using anti-ScFv expression system for prevention of tabacco mosaic virus (TMV) infection.Tabacco which was introduced with anti-DNARNA hybrid ScFv genes, expressed ScFv mRNA and showed marked resistance to TMV infection.3. Blocking experiment of human adult T cell leukemia virus (HTLV-1) infection by gene therapy using anti-ScFv expression system.We are establishing for evaluation of efficacy of ScFv strategy in order to block HTLV-1 infection in T cells.