Etiological agents responsible for systemic lupus erythrmatosus

1. 首先，我们证实了人单克隆抗dna抗体O-81可能是导致系统性红斑狼疮（SLE）组织损伤的肾源性抗体。我们建立SCID小鼠SLE模型，注射活性SLE淋巴细胞。抗81核酶的共存阻止了免疫沉积的形成和体内抗dna活性的升高。采用E coil-Pin Point TM系统对O-81 VH基因蛋白进行表达，获得O-81 scFV。O-81 scFV结合DNA和抗O-81独特型抗体。我们还获得了来自SLE淋巴细胞的cDNA文库。从SLE cDNA文库中获得的克隆上表达的肽，检测了其与o - 81scfv的结合能力。结果获得了16个克隆，部分克隆获得了重组蛋白。重组蛋白与O-81指示反应。这表明所测定的蛋白可能是SLE特异性的。

1. First, we confirmed that human monoclonal anti-DNA antibody O-81 would be nephritogenic antibodies responsible for tissue injury in systemic lupus erythematosus(SLE). We established SLE model in SCID mice, where active SLE lymphocytes were injected.The coexistence of anti-81 ribozyme prevented the formation of immune deposits and elevation of anti-DNA activity in vivo.2. O-81 scFV was obtained by using E coil-Pin Point TM system for the expression of protein from O-81 VH gene. O-81 scFV bound to DNA and anti-idiotypic antibodies to O-81. We also obtained cDNA library originated from SLE lymphocytes. The peptides expressing on the clones derived from SLE cDNA library, were examined for the binding ability to O-8 l scFV. As a result, 16 clones were obtained and the recombinant protein was obtained from some clones.3. The recombinant protein reacted with O-81 indicating. This indicates that the determined protein may be specific to SLE.