The pathological influence on ultrastructure of the red blood cell and the vascular system under long term circulation with continuous flow left heart bypass.

左心旁路连续流长期循环对红细胞超微结构和血管系统的病理影响。

基本信息

批准号：
13470280
负责人：
TAENAKA Yoshiyuki
金额：
$ 8.9万
依托单位：
National Cardiovascular Center Research Institute
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

项目摘要

1. Ultrastructural alterations in red blood cell (RBC) membranes exposed to shear stressWe investigated the ultrastructural alterations of RBC membrane with loaded shear stress. The result with continuous exposure to low shear stress showed an increase in band 3 density, and a decrease in the deformability of the RBC membrane. The results indicate the presence of another process leading to cell-fragmentation. The RBC membrane-skeleton with low shear stress shows delayed destruction.2. Aortic smooth muscle cells (SMC) under prolonged continuous flow left heart bypass (LHB).The goal of this study was to determine the roles of SMC in the vascular remodeling process in cases demonstrating aortic wall thinness under prolonged continuous flow LHB. These aortic SMC maintained contractile formation. These cells, however, synthesized redundant matrix metalloproteinase-2 & 9. The SMC demonstrated synthetic-dedifferentiated formation. In conclusion, aortic wall thinness under prolonged continuous … More flow LHB is caused by over-synthesis of matrix metalloproteinase in SMC, and these refers the vascular remodeling process of the extracellular matrix.3. The small artery under prolonged continuous flow LHBWe investigated morphological changes of the arterial systems in the kidneys under prolonged continuous flow LHB. The afferent arterioles were frequently thickened by an increase in the number of SMC after prolonged continuous flow LHB. Prolonged continuous flow LHB causes proliferation of SMC in the afferent arterioles in the kidneys.4. The brain under prolonged continuous flow LHBWe investigated the influence on the cerebral pathological findings under prolonged continuous flow LHB. The wall thickness of middle cerebral artery of the continuous flow group was not different from that of normal goats. The value of the vascular SMC contents and the properties of vascular SMC were also kept within normal range. The cerebral pathological findings and vascular systems under prolonged continuous flow LHB were within normal range. Less

1。暴露于剪切胁迫的红细胞（RBC）膜的超微结构改变研究了带有负载的剪切应力的RBC膜的超微结构改变。连续暴露于低剪切应力的结果显示，带3密度增加，RBC膜的可变形性降低。结果表明存在导致细胞碎片的另一个过程。带有低剪切应力的RBC膜骨骼显示延迟破坏。2。在延长的连续流左心旁路（LHB）下，主动脉平滑肌细胞（SMC）。本研究的目的是确定SMC在血管重塑过程中的作用，在延长连续流量LHB下的主动脉壁薄度。这些主动脉SMC保持收缩形成。但是，这些细胞合成了冗余基质金属蛋白酶-2和9。SMC表现出合成效力的形成。结论，长期连续的主动脉壁薄度……更多的流量LHB是由SMC中基质金属蛋白酶的过度合成引起的，这些是指细胞外基质的血管重塑过程。3。长时间连续流中的小动脉LHBWE研究了延长连续流LHB下肾脏中动脉系统的形态变化。延长连续流LHB后，SMC数量的增加通常会增厚传入小动物。延长的连续流LHB导致SMC在肾脏中的传入小动脉中的增殖。4。长时间连续流的大脑LHBWE调查了对延长连续流LHB下脑病理发现的影响。连续流动组中大脑中动脉的壁厚与正常山羊的壁厚度没有差异。血管SMC含量和血管SMC的性质的值也保持在正常范围内。在延长的连续流LHB下，脑病理发现和血管系统在正常范围内。较少的