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Experimental and comprehensive study to realize a nonpulsatile artificial heart

实现非搏动人工心脏的实验和综合研究

基本信息

批准号：
07557090
负责人：
TAENAKA Yoshiyuki
金额：
$ 5.82万
依托单位：
National Cardiovascular Center Research Institute
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

项目摘要

A centrifugal pump (CP) system were developed as an artificial heart for long term usage and pathophysiological reactions of living bodies to nonpulsatile systemic circulation were investigated in boty acute and chronic animal experiments. we developed a no-seal and no-shaft CP,NCVC-2, featuring self-blood-irrigation around the impeller for this purpose. The feasibility of the pump was assessed in terms of its performances including antithrombogenicity, durability, safety and implantablity as a long-term device and of physiological feasibility of nonpulsatile circulation. The size of the CP for the left ventricle was 60 mm in diameter and 100 mm in length, was implanted inside the chest of a goat and maintained 4-5 L/min and the temperature rise was within 2ﾟC.The CP was installed in goats as a paracorporeal left heart bypass and could be driven for 381 days in an animal, and proved to have good antithrombogenicity, durability and safety. The hemolysis rate of the CP was lower than Bio-pump in in vitro hemolysis tests. Physiological reaction to the nonpulsatile systemic and pulmonary circulation was evaluated in acute and anesthetized animal experiments and in awake animals. In the acute experimental setting, sympathetic nerve activity and blood noradrenalin increased immediately after the disappearance of pulsatility of systemic circulation but returned to the levels before the nonpulsatile circulation in a few minutes. Most of the parameters did not change in awake animals such as hemodynamic parameters, vasoactive substances, renin-angiotensin system and metabolic parameters with systemic nonpulsatile flow. Morphological changes of the aortic wall in prolonged nonpulsatile left heart bypass were investigated in a series of chronic animal experiments. The wall thickness of the aortic wall in nonpulsatile animals was significantly thinner and the volume ratio of the smooth muscle cells was lower.

研制了一种长期使用的人工心脏离心泵系统，并在急、慢性动物实验中观察了活体对非搏动性体循环的病理生理反应。为此，我们开发了一种无密封、无轴的CP，NCVC-2，其特点是在叶轮周围进行自我血液灌流。从抗凝血性、耐用性、安全性和作为长期装置的植入性以及非搏动性循环的生理可行性等方面对该泵的可行性进行了评估。左心室CP直径60 mm，长100 mm，植入山羊胸腔内，维持L/分钟4~5次/分钟，温升在2ﾟC以内。将CP安装在山羊体内作为体外左心转流，可在动物体内行驶381d，具有良好的抗凝血性、耐用性和安全性。在体外溶血实验中，CP的溶血率低于生物泵。在急性麻醉动物实验和清醒动物实验中，对非搏动性体循环和肺循环的生理反应进行了评估。在急性实验环境中，交感神经活动和血去甲肾上腺素在体循环搏动性消失后立即升高，但在几分钟内恢复到非搏动性循环前的水平。清醒动物的血流动力学参数、血管活性物质、肾素-血管紧张素系统和全身非搏动性血流代谢参数无明显变化。在一系列慢性动物实验中，观察了长时间非搏动性左心转流中主动脉壁的形态变化。非搏动性动物的主动脉壁厚度明显变薄，平滑肌细胞体积比明显降低。