Experimental and comprehensive study to realize a nonpulsatile artificial heart

实现非搏动人工心脏的实验和综合研究

基本信息

批准号：
07557090
负责人：
TAENAKA Yoshiyuki
金额：
$ 5.82万
依托单位：
National Cardiovascular Center Research Institute
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

项目摘要

A centrifugal pump (CP) system were developed as an artificial heart for long term usage and pathophysiological reactions of living bodies to nonpulsatile systemic circulation were investigated in boty acute and chronic animal experiments. we developed a no-seal and no-shaft CP,NCVC-2, featuring self-blood-irrigation around the impeller for this purpose. The feasibility of the pump was assessed in terms of its performances including antithrombogenicity, durability, safety and implantablity as a long-term device and of physiological feasibility of nonpulsatile circulation. The size of the CP for the left ventricle was 60 mm in diameter and 100 mm in length, was implanted inside the chest of a goat and maintained 4-5 L/min and the temperature rise was within 2ﾟC.The CP was installed in goats as a paracorporeal left heart bypass and could be driven for 381 days in an animal, and proved to have good antithrombogenicity, durability and safety. The hemolysis rate of the CP was lower than Bio-pump in in vitro hemolysis tests. Physiological reaction to the nonpulsatile systemic and pulmonary circulation was evaluated in acute and anesthetized animal experiments and in awake animals. In the acute experimental setting, sympathetic nerve activity and blood noradrenalin increased immediately after the disappearance of pulsatility of systemic circulation but returned to the levels before the nonpulsatile circulation in a few minutes. Most of the parameters did not change in awake animals such as hemodynamic parameters, vasoactive substances, renin-angiotensin system and metabolic parameters with systemic nonpulsatile flow. Morphological changes of the aortic wall in prolonged nonpulsatile left heart bypass were investigated in a series of chronic animal experiments. The wall thickness of the aortic wall in nonpulsatile animals was significantly thinner and the volume ratio of the smooth muscle cells was lower.

在Boty急性和慢性动物实验中，研究了一个人工使用和生物生理反应的人造心脏，用于长期使用和病理生理反应。为此，我们开发了一个无路和无轴CP，NCVC-2，其围绕着叶轮的自我灌溉。泵的可行性是根据其性能评估的，包括抗巩膜，耐用性，安全性和植入性，作为长期装置以及非脉冲循环的物理可行性。左心室的CP的大小直径为60毫米，长度为100毫米，植入了山羊胸部内，保持4-5 l/min，温度升高在2℃以内C. CP在山羊中安装在山羊体内左心脏旁路，并可以在动物中驱动381天，并且可以在动物中进行381天的态度，并在良好的情况下进行了良好的效果，并能够良好地和良性。 CP的溶血速率低于体外溶血测试的生物泵。在急性和麻醉的动物实验以及清醒的动物中评估了对非脉冲全身和肺循环的生理反应。在急性实验环境中，全身循环脉冲消失后，交感神经活动和血去甲肾上腺素立即增加，但在几分钟内无脉冲循环之前恢复了水平。大多数参数没有改变清醒的动物，例如血液动力学参数，血管活性物质，肾素 - 血管紧张素系统和具有全身性非脉冲流动的代谢参数。在一系列慢性动物实验中研究了长时间非乳突左心旁路的主动脉壁的形态变化。非乳突动物中主动脉壁的壁厚明显较薄，平滑肌细胞的体积比较低。