The neurochemical study of intra-operative awareness

术中意识的神经化学研究

• 批准号: 15591649
• 负责人: MURAKAWA Masahiro
• 金额: $ 1.79万
• 依托单位: Fukushima Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591649/
• 关键词: intraoprative awareness; acetylcholine; brain microdialysis; sevoflurane; isoflurane; enflurane; halothane

To clarify the neurochemical basis for intra-operative awareness, the changes of acetylcholine release by the painful stimulation in rat cerebral cortex during general anesthesia were studied using brain microdialysis. The contents in extracellar acetylcholine during anesthesia and after the 4 hours of 5% formalin injection subcutaneously at the hind paw were measured with high performance liquid chromatography.In non-anesthetized animals, the release of acetylcholine significantly increased to the twice of basal values with peak level at 30-45 minutes after formalin injection and then it gradually declined to the basal level. The acetylcholine release was significantly decreased by 1 MAC of halothane, isoflurane, enflurane, or sevoflurane anesthesia to 7, 16, 5, or 21% of basal values, respectively. However, no significant change of acetylcholine release was observed at the delivery of the pain stimulation under administration of these inhalation anesthetics.Acetylcholine is one of the major excitatory neurotransmitters in the central nervous system (CNS). Cholinergic neurons have been assumed to play an important role in regulating the state of consciousness, and it has been shown that acetylcholine release in the CNS decreases in the transition from wakefulness to sleep. Therefore, the results of this study provide the neurochemical basis for the fact that the occurrence of intra-operative awareness is less during the administration of inhalation anesthetics than intravenous one.

为了阐明术中意识的神经化学基础，使用脑微透析研究了大鼠脑皮质中乙酰胆碱释放的变化。用高性液相色谱法测量了麻醉期间的乙酰乙酰胆碱和4小时在后爪注射5％后的含量。在非动物中，乙酰胆碱的释放显着释放，在形式上逐渐变化的基础上，乙酰胆碱的释放显着增加到了两倍，并在形式上逐渐降低了峰值水平。乙酰胆碱释放的氟烷，异氟烷，enflurane或Sevoflurane麻醉分别分别为基础值的7％，16％，5或21％，显着降低了乙酰胆碱的释放。然而，在这些吸入麻醉药给药下的疼痛刺激下，乙酰胆碱释放没有明显变化。乙酰胆碱是中枢神经系统（CNS）中主要的兴奋性神经递质之一。假定胆碱能神经元在调节意识状态方面起着重要作用，并且已经表明，CNS中的乙酰胆碱释放在从觉醒到睡眠的过渡中降低。因此，这项研究的结果提供了一个神经化学基础，即在吸入麻醉药期间，术中意识的发生比静脉内的意识更少。

项目成果

Effects of magnesium on fos expression by histamine in rats spinal dorsal horn

镁对组胺大鼠脊髓背角fos表达的影响

• 发表时间: 2004
• 期刊: J Anesth 18(Suppl)
• 作者: Maru H;et al.
• 通讯作者: et al.