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Cytochemical and cell biological study on the mechanism of synthesis and action of neurosteroids in the olfactory bulb.

嗅球神经类固醇合成和作用机制的细胞化学和细胞生物学研究。

基本信息

批准号：
16590145
负责人：
ISHIMURA Kazunori
金额：
$ 2.24万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590145/
关键词：
Neurosteroids Olfactory bulb Steroid synthesizing enzymes 17β-Estradiol Glial cell

项目摘要

This study aims to clarify the mechanism of synthesis and action of neurosteroid in the rat and mouse olfactory bulbs, and to know the physiological roles of neurosteroids in this organ. The results obtained are as follows :1)Steroid synthesixzing enzymes (P450scc, 3beta-HSD, 17beta-HSD, P450c17, aromatase, 5alpha-reductase) and estrogen receptor (ER) were immunocytochemically localized in the olfactory bulb. The enzymes except 5alpha-reductase and ER were present in neurons but 5alpha-reductase was present in glial cells.2)The results of RT-PCR, Northern-blotting, and Western-blotting showed the presence of the enzymes and ER at the DNA, mRNA and pretein level in the olfactory bulb.3)The enzyme actibvities of P450scc, 17beta-HSD and 5alpha-reductase were demonstrated in the olfactory bulb.4)Whe mice were administered with estradiol, the ecpressions of P450scc, P450c17, 3beta-HSD and aromatase were suppressed in the olfactory bulb. However, the expression of the other enzymes and ER did not change.5)Olfactory ensheathing cells were themain 5alpha-reductase immunopositive cells in the olfactory nerve layer of the olfactory bulb. However, in the other layer of the olfactory bulb, the 5alpha-reductase immunopositive cells were astrocytes and oligodendorcytes.6)In order to compare the results, immunohisitochemistry was achieved in the cerebellum and hippocampus. The results showed that 5alpha-reductase was present in astrocytes and oligodendrocytes in these brain areas, too.7)When mice were treated with estradiol or progesterone, mRNAs of P450c17 and aromatase in the olfactory bulb were decreased, but they were increased by the treatment with dihydorotestosterone.8)The present results showed that all the enzymes required for the sex-steroid synthesis were present in the olfactory bulb, and that the expression of the enzymes was controlled by sex-steroids.

本研究旨在阐明神经甾体在大鼠和小鼠嗅球中的合成和作用机制，了解神经甾体在嗅球中的生理作用。结果如下：1）类固醇合成酶（P450 SCC、3 β-HSD、17 β-HSD、P450 C17、芳香化酶、5 α-还原酶）和雌激素受体（ER）在嗅球中的免疫细胞化学定位。2）RT-PCR、Northern-blotting和Western-blotting结果表明，嗅球中除5 α-还原酶和ER外，其余酶均存在于神经元中，而5 α-还原酶仅存在于胶质细胞中。4）雌二醇可抑制小鼠嗅球中P450 cc、P450 c17、3 β-HSD和芳香化酶的分泌。5）嗅鞘细胞是嗅球嗅神经层中主要的5 α-还原酶免疫阳性细胞。而在嗅球的另一层中，5 α-还原酶免疫阳性细胞为星形胶质细胞和寡树突细胞。6）为了比较结果，在小脑和海马中进行免疫组织化学。结果表明，5 α-还原酶也存在于这些脑区的星形胶质细胞和少突胶质细胞中。7）当小鼠被雌二醇或孕酮处理时，嗅球中P450 c17和芳香化酶的mRNA减少，而被二氢睾酮处理时，它们增加。8）目前的结果表明，性类固醇合成所需的所有酶都存在于嗅球中，这些酶的表达受性类固醇激素的控制。

项目成果

期刊论文数量（35）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Localization of enolase in synaptic plasma membrane as an alphagamma heterodimer in rat brain.

烯醇化酶在大鼠脑中作为αγ异二聚体在突触质膜中的定位。

DOI：
发表时间：
2004
期刊：
Neuroscience Research vol.48
影响因子：
0
作者：
Terada N;Ohno N;Yamakawa H;Ohara O;Ohno S.;H.Ueta et al.
通讯作者：
H.Ueta et al.

The localization of steroid synthesizing enzymes in the olfactory bulb.

类固醇合成酶在嗅球中的定位。

DOI：
发表时间：
2004
期刊：
Shikokuigakuzasshii(in Japanese) vol.59
影响因子：
0
作者：
Hiroaki Y;Tani K;Kamegawa A;Gyobu N;Nishikawa K;Suzuki H;Walz T;Sasaki S;Mitsuoka K;Kimura K;Mizoguchi A;Fujiyoshi Y.;E.Kiyokage et al.
通讯作者：
E.Kiyokage et al.

嗅球におけるステロイド合成酵素の局在

嗅球中类固醇生成酶的定位

DOI：
发表时间：
2004
期刊：
四国医学雑誌 59・4-5
影响因子：
0
作者：
Makoto Kadowaki;Hirofumi Kuramoto;Miyako Takaki;清蔭恵美他
通讯作者：
清蔭恵美他

Src and Cas are essentially but defferentially involved in angiotensin II-stimulated migration of vascular smooth muscle cells via extracellular signal-regulated kinase 1/2 and c-Jun NH2-terminal kinase activation.

Src 和 Cas 通过细胞外信号调节激酶 1/2 和 c-Jun NH2 末端激酶激活，本质上但又不同地参与血管紧张素 II 刺激的血管平滑肌细胞迁移。

DOI：
发表时间：
2004
期刊：
Molecular Pharmacology 65・4
影响因子：
0
作者：
Ohno N;Terada N;Tanaka J;Yokoyama A;Yamakawa H;Fujii Y;Baba T;Ohara O;Ohno S.;M.Kyaw et al.
通讯作者：
M.Kyaw et al.

Genetic background markedly influences vulnerability of the hippo-campal neuronal organization in the twitcher mouse.

遗传背景显着影响抽搐小鼠海马神经元组织的脆弱性。