Cytochemical analysis on the mechanisms of biosynthesis and action of steroid hormones.

类固醇激素生物合成和作用机制的细胞化学分析。

• 批准号: 07670019
• 负责人: ISHIMURA Kazunori
• 金额: $ 1.47万
• 依托单位: The University of Tokushima, School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670019/
• 关键词: 5 alpha-Reductase; Immunocytochemistry; Electron microscope; Steroid hormones; Anterior pituitary; Prostate; Adrenal gland; Schwann cells; 中枢神経; 精管; 脳; アロマターゼ

(1) Immunocytochemical localization of 5 alpha-reductase was studied in various tissues using polyclonal antibodies against type 1 and 2 isozymes.(2) In the rat anterior pituitary, type 1 isozyme was present in the membrane of rough endoplasmic reticulum of gonadotrophs.(3) In the rat adrenal cortex, zonae fasciculata-reticularis cells contain 5 alpha-reductase type 1. The expression of the enzyme increased in the castrated animals. This indicates that sex steroids play a role in the regulation of corticoids secretion and metabolism.(4) In the rat adrenal medulla, 5 alpha-reductase type 1 was present in the supporting cells surrounding chromaffin cells and Schwann cells around nerve fivers.(5) Steroid 5 alpha-reductase was present in the satellite cells in the ganglions in addition to Schwann cells.(6) In the human prostate, seminal vesicle and vas deferens, type 2 isozyme was present in the basal epithelial cells and smooth muscle cells in the interstitial tissue.(7) Leucocyte type arachidonate 12-lipoxygenase was immunolocalized in the secretory cells of pig anterior pituitary gland and neutrophils of dog.(8) Platelet type arachidonate 12-lipoxygenase was immunocytochemically localized in the plateletes and eosinophils of the peripheral blood, megakaryocytes of the bone marrow, and keratinocytes of the epidermis.

(1)使用抗1型和2型同工酶的多克隆抗体研究了5 α-还原酶在各种组织中的免疫细胞化学定位。(2)在大鼠垂体前叶，促性腺激素细胞粗面内质网膜上存在1型同工酶。(3)在大鼠肾上腺皮质中，束状带网状细胞含有1型5 α-还原酶。酶的表达在去势动物中增加。这表明性类固醇激素在调节皮质激素分泌和代谢中起作用。(4)在大鼠肾上腺髓质，5 α-还原酶1型存在于嗜铬细胞周围的支持细胞和神经纤维周围的雪旺细胞。(5)类固醇5 α-还原酶存在于除了雪旺细胞的神经节中的卫星细胞。(6)在人前列腺、精囊和输精管中，2型同工酶主要分布于基底上皮细胞和间质平滑肌细胞。(7)白细胞型花生四烯酸12-脂氧合酶免疫定位于猪垂体前叶分泌细胞和狗中性粒细胞。(8)血小板型花生四烯酸12-脂氧合酶免疫细胞化学定位于血小板和嗜酸性粒细胞的外周血，骨髓的巨核细胞，表皮的角质形成细胞。

项目成果

H.Ikawa,K.Yamamoto,K.Ishimura: "Arachidonate 12-lipoxygenase in porcine anterior pituitary cells :" Journal of Endocrinology. 148. 33-41 (1996)

H.Ikawa、K.Yamamoto、K.Ishimura：“猪垂体前叶细胞中的花生四烯酸 12-脂氧合酶：”内分泌学杂志。

Y.Tsuruo,T.Miyamoto,K.Ishimura: "Immunohistochemical presence of 5α-reductase type 1-containing cell in the rat brain." Brain Research. 722. 207-211 (1996)

Y.Tsuruo、T.Miyamoto、K.Ishimura：“大鼠脑中含有 5α-还原酶 1 型细胞的免疫组织化学存在。” 722. 207-211 (1996)

Y.Tsuruo,K.Ishimura: "Immunohistochemical localization of estrogen receptors within" Anatomy and Embryology. 193. 115-121 (1996)

Y.Tsuruo，K.Ishimura：“解剖学和胚胎学中雌激素受体的免疫组织化学定位”。

K.Ishimura and H.Fujita: "Further observations on the fine structure of the development of the interrenal tissue of the chick embryo." Tokushima J.of Experimental Medicine. 42. 17-26 (1995)

K.Ishimura 和 H.Fujita：“鸡胚肾间组织发育精细结构的进一步观察”。

Ikawa, H.: "Arachidonate 12-lipoxygenase in porcine anterior pituitary cells: its localization and possible function in gonadotrophs." Journal of Endocrinology. 148. 33-41 (1996)

Ikawa, H.：“猪垂体前叶细胞中的花生四烯酸 12-脂氧合酶：其在促性腺激素中的定位和可能的功能。”