Molecular biological and cytochemical study on the mechanisms of expression of steroid hormone synthesizing enzymes.

类固醇激素合成酶表达机制的分子生物学和细胞化学研究。

• 批准号: 11670013
• 负责人: ISHIMURA Kazunori
• 金额: $ 1.86万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670013/
• 关键词: Steroid hormones; 17β-Hydroxysteroid dehydrogenase; 17α-Hydroxylase; C17-20 lyase; 5α-Reductase; Cytochemistry; Electron microscopy; Prostaglandin; Spinal cord; 前立腺; 17β-ヒドロキシステロイドデヒドロゲナーゼ; 免疫組織化学; 17α水酸化; C17-20リア-ゼ; チトクロームb5; プロスタグランジンF合成

(1) We got polyclonal antibodies against 3β-and 17β-hydroxysteroid dehydrogenases. They are applicable for immunohistochemistry.(2) 17α-Hydroxylase/C17-20 lyase was detected in rat gastric parietal cells and pituitary intermediate lobe cells by means of immunohistochemistry and Western blotting.(3) Aromatase, 17β-hydroxysteroid dehydrogenase and 5α-reductase were immunohistochemically detected in rat olfactory bulb.(4) Dexamethasone enhances serum deprivation-induced necrotic death of rat C6 glioma cells through activation of glucocorticoid receptors. The appearance of 5α-reductase immunoreactivity in the cytoplasm of C6 glioma cells under serum-free conditions was probably not due to changes in 5α-reductase gene expression.(5) Liver type prostaglandin F (PGF) synthase was immunocytochemically localized in the sinusoidal endothelial cells of rat liver.(6) In the rat spinal cord, lung type PGF synthase was immunolocalized in the dendrites of nerve cells and vascular endothelial cells, and liver type one in the tanycytes and vascular endothelial cells.(7) We could not set good antibody against cytochrome b5 which plays important roles in the contorl of function of 17α-hydroxylase/C17-20 lyase.

(1)获得了3个β-和17个β-羟基类固醇脱氢酶的多克隆抗体。(2)免疫组织化学和免疫印迹法检测到17α-羟基酶/C17-20裂解酶在大鼠胃壁细胞和垂体中间叶细胞中表达，(3)在大鼠嗅球中表达芳香化酶、17β-羟基类固醇脱氢酶和5-α-还原酶。(4)地塞米松通过激活糖皮质激素受体而增强血清剥夺诱导的大鼠C6胶质瘤细胞的坏死性死亡。(5)肝型前列腺素F(α)合成酶免疫细胞化学定位于大鼠肝脏的肝窦内皮细胞。(6)在大鼠脊髓中，肺型前列腺素F合成酶定位于神经细胞和血管内皮细胞的树突中，而肝型前列腺素F合成酶定位于神经细胞和血管内皮细胞的树突中。(7)我们无法针对细胞色素b5产生良好的抗体，该抗体在17α羟基酶/C17 20裂解酶的功能调控中起重要作用。

项目成果

Yamamoto S: "Fatty acid cyclooxygenase induction accompanied by prostaglandin Dsynthesis in a human megakaryoblastic cell line CMK differentiated by phorbolester."Biofactors. 11. 57-61 (2000)

Yamamoto S：“在由佛波酯分化的人巨核细胞系 CMK 中，脂肪酸环氧合酶诱导伴随前列腺素合成。”生物因子。

Yamamoto S: "Lipoxygenases and Their Metabolites, edited by Nigam and Pace-Asciak."Plenum Press, New York. 8 (1999)

Yamamoto S：“脂氧合酶及其代谢物，Nigam 和 Pace-Asciak 编辑。”Plenum Press，纽约。

Suzuki-Yamamoto T: "Identification of prostaglandin F-producing cells in the liver."Histochem Cell Biol. 112. 451-456 (1999)

Suzuki-Yamamoto T：“鉴定肝脏中前列腺素 F 生成细胞。”组织化学细胞生物学。

Yamamoto S: "Fatty acid cyclooxygenase induction accompanied by prostaglandin Dsynthesis in a human megakaryoblastic cell line CMK differentiated by phorbol ester."Biofactors. 11. 57-61 (2000)

Yamamoto S：“在佛波酯分化的人巨核细胞系 CMK 中，脂肪酸环氧合酶诱导伴随前列腺素合成。”生物因子。

Matsuda J: "Developmental changes in long-form leptin receptor expression and localization in rat brain."Endocrinology. 140. 5233-5238 (1999)

Matsuda J：“大鼠大脑中长型瘦素受体表达和定位的发育变化。”内分泌学。