Cytochemical and molecular biological study on the control mechanisms of differentiation and function of steroid hormone synthesizing cells.

(1)免疫组织化学研究5-α-还原酶在犬前列腺腺上皮细胞中的定位。(2)在大鼠脊髓，肺型前列腺素F合成酶免疫定位于神经细胞和血管内皮细胞的树突和胞体。(3)17β-羟基类固醇脱氢酶免疫定位于大鼠嗅球神经细胞。(4)为了获得人VH的人工抗体，我们制作了表达人VH的噬菌体文库。(5)未能获得参与肾上腺皮质细胞分化的因子。(6)用免疫细胞化学和酶组织化学方法研究了老年和糖尿病大鼠主要盆神经节中一氧化氮合酶的表达。结果表明，Ghrelin在两组大鼠神经节中的表达均降低，以糖尿病大鼠为甚。(7)对围生期大鼠Ghrelin的表达进行了生化和免疫细胞化学研究。围产期大鼠血Ghrelin水平较高。Ghrelin产生的主要部位位于胎盘合体滋养层细胞。出生后，胃促生长素的产生部位是胃腺的内分泌细胞。

(1) Immunohistochemical studies localized 5α-reductase in the glandular epithelial cells of the canine prostate. After the treatment of an anti-androgenic drug, the immunoreactivity of the cells was decreased.(2) In the rat spinal cord, lung type PGF synthase was immunolocalized in the dendrites and cell bodies of nerve cells and vascular endothelial cells.(3) 17β-Hydroxysteroid dehydrogenase was immunolocalized in the nerve cells of the rat olfactory bulb.(4) In order to obtain an artificial antibody, we made a phage library expressing human VH.(5) We could not get factors involved in the differentiation of adrenal cortical cells.(6) The expression of NO synthase is studied in the major pelvic ganglia of aged and diabetic rats by means of immunocytochemistry and enzyme histochemistry. The results indicated that the expression of the enzyme was decreased in the ganglia of the both rats, especially in the diabetic one.(7) The expression of ghrelin was studied biochemically and immunocytochemically in the perinatal rats. The blood ghrelin level was high in the perinatal rats. The major site of the ghrelin production was immunolocalized in the syncytiotrophoblast of placenta. After birth, the site of ghrelin production was endocrine cells of the gastric glands.