Cytochemical and molecular biological study on the control mechanisms of differentiation and function of steroid hormone synthesizing cells.

类固醇激素合成细胞分化和功能控制机制的细胞化学和分子生物学研究。

• 批准号: 13670016
• 负责人: ISHIMURA Kazunori
• 金额: $ 2.5万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670016/
• 关键词: adrenal cortex; 17β- Hydroxysteroid dehydrogenase; immunohistochemistry; 5α-Reductase; Differentiation; Rat; Prostaglandin; Phage library; 抗体; バクテリオファージ; NO合成酵素; ghrelin; 胎盤; ステロイドホルモン; 電子顕微鏡; 副腎; 前立腺

(1) Immunohistochemical studies localized 5α-reductase in the glandular epithelial cells of the canine prostate. After the treatment of an anti-androgenic drug, the immunoreactivity of the cells was decreased.(2) In the rat spinal cord, lung type PGF synthase was immunolocalized in the dendrites and cell bodies of nerve cells and vascular endothelial cells.(3) 17β-Hydroxysteroid dehydrogenase was immunolocalized in the nerve cells of the rat olfactory bulb.(4) In order to obtain an artificial antibody, we made a phage library expressing human VH.(5) We could not get factors involved in the differentiation of adrenal cortical cells.(6) The expression of NO synthase is studied in the major pelvic ganglia of aged and diabetic rats by means of immunocytochemistry and enzyme histochemistry. The results indicated that the expression of the enzyme was decreased in the ganglia of the both rats, especially in the diabetic one.(7) The expression of ghrelin was studied biochemically and immunocytochemically in the perinatal rats. The blood ghrelin level was high in the perinatal rats. The major site of the ghrelin production was immunolocalized in the syncytiotrophoblast of placenta. After birth, the site of ghrelin production was endocrine cells of the gastric glands.

(1)免疫组织化学研究5-α-还原酶在犬前列腺腺上皮细胞中的定位。(2)在大鼠脊髓，肺型前列腺素F合成酶免疫定位于神经细胞和血管内皮细胞的树突和胞体。(3)17β-羟基类固醇脱氢酶免疫定位于大鼠嗅球神经细胞。(4)为了获得人VH的人工抗体，我们制作了表达人VH的噬菌体文库。(5)未能获得参与肾上腺皮质细胞分化的因子。(6)用免疫细胞化学和酶组织化学方法研究了老年和糖尿病大鼠主要盆神经节中一氧化氮合酶的表达。结果表明，Ghrelin在两组大鼠神经节中的表达均降低，以糖尿病大鼠为甚。(7)对围生期大鼠Ghrelin的表达进行了生化和免疫细胞化学研究。围产期大鼠血Ghrelin水平较高。Ghrelin产生的主要部位位于胎盘合体滋养层细胞。出生后，胃促生长素的产生部位是胃腺的内分泌细胞。

项目成果

石村和敬: "看護のための最新医学講座:人体の構造と機能(分担)"中山書店. 420 (2002)

石村和隆：“最新护理医学课程：人体的结构和功能（分区）”中山书店420（2002）。

Nakamura M, Watanabe H, Nishimiya Y, Tsumoto K, Ishimura K, Kumagai I: "Panning of a phage VH library using nitrocellulose membranes : Application to selection of a human VH library."J Biochem. 129. 209-212 (2001)

Nakamura M、Watanabe H、Nishimiya Y、Tsumoto K、Ishimura K、Kumagai I：“使用硝酸纤维素膜淘选噬菌体 VH 文库：在选择人 VH 文库中的应用。”J Biochem。

北村聖子: "周産期におけるラットGhrelinの血中濃度及び遺伝子発現の検討"ホルモンと臨床. 50. 1178-1200 (2002)

Seiko Kitamura：“围产期大鼠 Ghrelin 的血液浓度和基因表达的检查”激素和临床研究 50. 1178-1200 (2002)。

Tsuji A, Sakurai K, Kiyokage E, Yamazaki T, Koide S, Toida K, Ishimura K, Matsuda Y: "Secretory protein convertases PACE4 and PC&A are heparin-binding proteins which are localized in the extracellular matrix : Potential role of PACE4 in the activation of

Tsuji A、Sakurai K、Kiyokage E、Yamazaki T、Koide S、Toida K、Ishimura K、Matsuda Y：“分泌蛋白转化酶 PACE4 和 PC

石村 和敬: "看護のための最新医学講座:人体の構造と機能(分担)"中山書店. 420 (2002)

石村和隆：“最新护理医学课程：人体的结构和功能（分区）”中山书店420（2002）。