Cytochemical and molecular biological analysis on the mechanisms of production and action of neurosteroids in the mouse cerebellum.

小鼠小脑神经类固醇产生和作用机制的细胞化学和分子生物学分析。

• 批准号: 18590185
• 负责人: ISHIMURA Kazunori
• 金额: $ 2.47万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590185/
• 关键词: cerebellum; neurosteroid; immunocytochemistry; receptor; Bergmann glia; Purkinje cell; 5α-reductase; enzyme expression; 神経ステロイ; 酵素; 細胞化学; 分子生物学; 培養; 局在; 発現

This study aims to clarify the mechanism of synthesis and action of neurasteroids in tha mouse cerebellum, and to know the physiological roles of neurasteroids in this organ. Theresults obtained areas follows:1) Steroids synthesizing enzymes(SCC, 3beta-HSD, 17beta-HSD, P450c17, aromatase, 5alfa-reductase) were detected in mRNA level by PCR method and protein level by Western blotting. The results or immunocytochemistry indicated that 5alfa-reductase was present in the Bergmann glia and the other enzymes were in the Purkinje cells.2) The expression of 5alfa-reductase was immunocytochmically studied in the neonatal mice cerebellum. The enzyme was detected in the 1 week-old mice cerebellum. The outer granular layer is formed between 1 and 3 weeks after birth. The expression of 5alfa-reductase is well coincided with this formation of the layer.3) These results indicated that the expression of the steroid synthesizing enzyme are intimately associated with the formation of the granular layer in the cerebellum and radial migration of neurons in this layer.

本研究旨在阐明小鼠小脑中神经类固醇的合成和作用机制，了解神经类固醇在该器官中的生理作用。获得的结果如下：1)采用PCR法检测类固醇合成酶(SCC、3β-HSD、17β-HSD、P450c17、芳香酶、5α-还原酶)的mRNA水平和Western blotting的蛋白水平。免疫细胞化学结果表明5α还原酶存在于伯格曼胶质细胞中，其他酶存在于浦肯野细胞中。2)免疫细胞化学方法研究了新生小鼠小脑中5α还原酶的表达。在1周大的小鼠小脑中检测到该酶。外颗粒层在出生后 1 至 3 周内形成。 5α-还原酶的表达与该层的形成非常吻合。3)这些结果表明类固醇合成酶的表达与小脑颗粒层的形成和该层神经元的径向迁移密切相关。

项目成果

Colocalization of prostaglandin F synthase,cyclooxigenase-1 and prostaglandin F receptor in mouse Leydig cells

小鼠 Leydig 细胞中前列腺素 F 合酶、环氧合酶-1 和前列腺素 F 受体的共定位

• 发表时间: 2007
• 期刊: Histochemistry and Cell Biology 128
• 作者: Matsutani T;Ohmori T;Ogata M;Soga H;Itoh T.;Suzuki-Yamamoto T
• 通讯作者: Suzuki-Yamamoto T

Vesicular acetylcholine transporter-immunoreactive axon terminals enriched in the pontine nuclei of the mouse

小鼠脑桥核中富集的囊泡乙酰胆碱转运蛋白免疫反应性轴突末端

• 发表时间: 2007
• 期刊: Neuroscience 146
• 作者: 渡部剛;伊藤恵美;阪井裕子;渡辺慎哉;Tsutsumi T
• 通讯作者: Tsutsumi T

Prostate stem cell antigen predicts tumour recurrence in superficial transitional cell carcinoma of the urinary bladder

• DOI: 10.1111/j.1464-410x.2006.06153.x
• 发表时间: 2006-06-01
• 期刊: BJU INTERNATIONAL
• 影响因子: 4.5
• 作者: Elsamman, Essam;Fukumori, Tomoharu;Kanayama, Hiro-Omi
• 通讯作者: Kanayama, Hiro-Omi

Effects of experimentally induced allergic rhinitis on the mouse olfactory system ; electrophysiological and histological studies.

实验诱导的过敏性鼻炎对小鼠嗅觉系统的影响；

• 发表时间: 2007
• 期刊: Nagoya Medical Journal 48
• 作者: Sakabe M;Matsui H;Sakata H;Ikeda K;Yamagishi T;Nakajima Y.;佐久間 信至;Shinji Sakuma;日渡 謙一郎;Ken-ichiro Hiwatari;Ken-ichiro Hiwatari;Ken-ichiro Hiwatari;佐久間 信至;Shinji Sakuma;Kiyoko Iwata;Kiyoko Iwata;Kiyoko Iwata;Kiyoko Iwata;岩田 記代子;Kiyoko Iwata;岩田 記代子;Kiyoko Iwata;Shinji Sakuma;Shinji Sakuma;Ken-ichiro Hiwatari;Ken-ichiro Hiwatari;Suzuki-Yamamoto T;Toida K;Masaki Ogata;Toida K
• 通讯作者: Toida K

Blood vessels form a scaffold for neuroblast migratior in the adult olfactory bulb.

血管形成成人嗅球中神经母细胞迁移的支架。

• 发表时间: 2007
• 期刊: The Journal of Neuroscience 27
• 作者: 渡部剛;他;Toida K
• 通讯作者: Toida K