Immunocytochemical Study on the Mechanism of Biosynthesis and Secretion of Steroid Hormones

类固醇激素生物合成与分泌机制的免疫细胞化学研究

• 批准号: 01570011
• 负责人: ISHIMURA Kazunori
• 金额: $ 1.34万
• 依托单位: The University of Tokushima (1990)Osaka University (1989)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570011/
• 关键词: Immunocytochemistry; Ovary; Testis; Aromatase; 17alpha-Hydroxylase; C17-C20lyase; Estrogen; Androstenedione; Ovulation; 排卵; 17αー水酸化酵素@C_<17>ーC_<20>リア-ゼ; 内卵胞膜細胞; 間質腺細胞; アンドロテンジオン

1. Light microscopic immunocytochemistry demonstrated that aromatase, an estrogen synthesizing enzyme, is present in the granulosa cells of mature follicles of the ovaries of rats, mice, hamsters, guinea pigs and cows.2. Immunocytochemical studies on the localization of aromatase revealed that in immature rat ovaries treated with PMSG and hCG, the reaction of the granulosa cells of the antral follicles against anti-aromatase antibody became strongly positive about 12 h before ovulation and then became very weak suddenly after ovulation.3. Aromatase is immunocytochemically demonstrated in the pregnant corpora lutea after day 7 of pregnancy.4. The localization of 17alpha-hydroxylase/C17-C20 lyase was immunocytochemically studied in the ovaries of immature rats treated with PMSG and hCG. The enzyme was present in the theca interna cells and interstitial gland cells of the rat ovaries 48 h after the PMSG treatment. After hCG injection, immunoreactive cells decreased in number, and no positive cells was detected 12 h after the hCG injection. Forty-eight hours after the hCG injection, the cells of theca interna and interstitial glands became immunoposive again.5. Immunocytochemical studies showed that aromatase was present in Leydig cells of equine testes.

1.光镜免疫细胞化学证实芳香化酶是一种雌激素合成酶，存在于大鼠、小鼠、仓鼠、豚鼠和牛卵巢成熟卵泡的颗粒细胞中.芳香化酶的免疫细胞化学定位研究显示，PMSG和hCG处理的未成熟大鼠卵巢中，窦卵泡颗粒细胞对抗芳香化酶抗体的反应在排卵前12 h左右呈强阳性，排卵后突然变弱.芳香化酶在妊娠第7天后的妊娠黄体中被免疫细胞化学证实。采用免疫细胞化学方法研究了PMSG和hCG处理的未成熟大鼠卵巢中17 α-羟化酶/C17-C20裂合酶的定位。PMSG处理48 h后，大鼠卵巢内膜细胞和间质腺细胞中存在该酶。注射hCG后免疫反应阳性细胞数量减少，12 h后未检测到阳性细胞。注射hCG后48小时，内膜和间质腺细胞再次呈免疫阳性反应.免疫细胞化学研究表明，芳香化酶存在于马睾丸间质细胞。

项目成果

T.Senda: "Ultrastructural and immunocytochemical studies on the cytoskeleton in the anterior pituitary of rats,with special regard to the relationship between actin filaments and secretory granules." Cell Tissue Res. 258. 25-30 (1989)

T.Senda：“对大鼠垂体前叶细胞骨架的超微结构和免疫细胞化学研究，特别关注肌动蛋白丝和分泌颗粒之间的关系。”

石村 和敬: "ステロイド産生機構の組織学" 内分泌学の進歩. 8.

Kazutaka Ishimura：“类固醇生成机制的组织学”内分泌学进展 8。

H.Matsubara: "T cell clones are killed by a thymic stromal cell monolayer following stimulation of T cell receptor with antigen and/or Hー2 molecules on the monolayer." Int.Immunol.2. 755-763 (1990)

H.Matsubara：“用单层上的抗原和/或 H-2 分子刺激 T 细胞受体后，T 细胞克隆被胸腺基质细胞单层杀死。”Int.Immunol.2 (1990)。

T.YoshinagaーHirabayashi: "Immunocytochemical localization of aromatase in immature rat ovaries treated with PMSG and hCG,and in pregnant rat ovaries." Histochemistry. (1989)

T. Yoshinaga-Hirabayashi：“用 PMSG 和 hCG 处理的未成熟大鼠卵巢和怀孕大鼠卵巢中芳香酶的免疫细胞化学定位”（1989）。

H.Fujita: "Secretory mechanisms of endocrine cells." Gunma Symposia on Endocrinology. 26. 9-24 (1989)

H.Fujita：“内分泌细胞的分泌机制。”