Immunocytochemical study on the mechanism of steroid hormone biosynthesis.
类固醇激素生物合成机制的免疫细胞化学研究。
基本信息
- 批准号:61570011
- 负责人:
- 金额:$ 1.6万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (C)
- 财政年份:1986
- 资助国家:日本
- 起止时间:1986 至 1988
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
1.(1) Electron microscopic immunocytochemistry using specific antisera revealed that 3 beta-hydroxysteroid dehydrogenase is present on the membranes of smooth endoplasmic reticulum of the bovine adrenocortical cells. This result indicate that the conversion of pregnenolone to progesterone occurs on the smooth endoplasmic reticulum of the adrenocortical cells. (2) Electron microscopic immunocytochemistry revealed that ATP synthase is present on the inner mitochondrial membranes.2. Light microscopic immunocytochemistry demonstrated that aromatase (estrogen synthesizing enzyme) is present in the granulosa cells of preovulatory large follicles and in the some cells of corpus luteum, and that 17 betahydroxysteroid dehydrogenase which converts androstenedione to testosterone is present in the cells of theca interna and interstitial glands.3. Cytochrome p-450 which is involved in the detoxication of some chemical compounds could be localized immunocyotchemically in the steroid-secreting cells of the ovary and testis of 3-methylcholanthrene-treated mice.4. Calspectin which undercoats the plasma membrane is shown immunocytochemically at the baso-lateral plasma membrane of thyroid follicle epithelial cells of normal rats. In the follicle epithelial cells of TSH-treated rats, calspectin is demonstrated at the apical plasma membrane in addtion to the baso-lateral plasma membrane.5. Aromatage (estrogen synthesizing enzyme) is demonstrated in the corpus luteum cells of pregnant rats. This result indicate that the corpus luteum cells of the pregnant rats may have the capacity of estrogen synthesis.
1. (1)使用特异性抗血清的电子显微镜免疫细胞化学显示,3 β-羟基类固醇脱氢酶存在于牛肾上腺皮质细胞的滑面内质网膜上。这一结果表明,去甲烯醇酮转化为孕酮发生在肾上腺皮质细胞的滑面内质网上。(2)电镜免疫细胞化学显示ATP合成酶存在于线粒体内膜上.光镜免疫细胞化学显示,排卵前大卵泡的颗粒细胞和黄体的部分细胞中存在芳香化酶(雌激素合成酶),内膜和间质腺细胞中存在将雄烯二酮转化为睾酮的17 β-羟基类固醇脱氢酶.细胞色素p-450参与了某些化合物的解毒作用,免疫细胞化学法可定位于3-甲基胆蒽处理的小鼠卵巢和睾丸的类固醇分泌细胞.免疫细胞化学显示,正常大鼠甲状腺滤泡上皮细胞的基底外侧质膜上有钙荧光素(Calspectin),它包被在质膜下面。在促甲状腺激素处理的大鼠卵泡上皮细胞中,除了基底外侧质膜外,还发现了顶端质膜上的钙特异蛋白.芳香化酶(雌激素合成酶)在妊娠大鼠的黄体细胞中得到证实。结果提示,孕鼠黄体细胞可能具有雌激素合成能力。
项目成果
期刊论文数量(41)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
M. Kurosumi; K. Ishimura; T. Yoshinaga; H. Fujita; B. Tamaoki: "Immunocytochemical localization of 17-beta-hydroxysteroid dehydrogenase in porcine testis." Histochemistry. 85. 287-289 (1986)
M.黑角;
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
K.Ishimura: Cell and Tissue research. 245. 681-683 (1986)
K.Ishimura:细胞和组织研究。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
ISHIMURA Kazunori其他文献
ISHIMURA Kazunori的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('ISHIMURA Kazunori', 18)}}的其他基金
Histochemical and biochemical study of the production and function of neurosteroid
神经类固醇的产生和功能的组织化学和生化研究
- 批准号:
23590236 - 财政年份:2011
- 资助金额:
$ 1.6万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Cytochemical and molecular biological analysis of neurosteroid synthesis and action mechanism in the hippocampus
海马神经甾体合成及作用机制的细胞化学和分子生物学分析
- 批准号:
20590192 - 财政年份:2008
- 资助金额:
$ 1.6万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Cytochemical and molecular biological analysis on the mechanisms of production and action of neurosteroids in the mouse cerebellum.
小鼠小脑神经类固醇产生和作用机制的细胞化学和分子生物学分析。
- 批准号:
18590185 - 财政年份:2006
- 资助金额:
$ 1.6万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Cytochemical and cell biological study on the mechanism of synthesis and action of neurosteroids in the olfactory bulb.
嗅球神经类固醇合成和作用机制的细胞化学和细胞生物学研究。
- 批准号:
16590145 - 财政年份:2004
- 资助金额:
$ 1.6万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Cytochemical and molecular biological study on the control mechanisms of differentiation and function of steroid hormone synthesizing cells.
类固醇激素合成细胞分化和功能控制机制的细胞化学和分子生物学研究。
- 批准号:
13670016 - 财政年份:2001
- 资助金额:
$ 1.6万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Molecular biological and cytochemical study on the mechanisms of expression of steroid hormone synthesizing enzymes.
类固醇激素合成酶表达机制的分子生物学和细胞化学研究。
- 批准号:
11670013 - 财政年份:1999
- 资助金额:
$ 1.6万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Cytochemical study on the mechanisms of expression and function of steroid hormone synthesizing enzymes.
类固醇激素合成酶表达和功能机制的细胞化学研究。
- 批准号:
09670016 - 财政年份:1997
- 资助金额:
$ 1.6万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Cytochemical analysis on the mechanisms of biosynthesis and action of steroid hormones.
类固醇激素生物合成和作用机制的细胞化学分析。
- 批准号:
07670019 - 财政年份:1995
- 资助金额:
$ 1.6万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Immunohistochemical analysis of steroid hormone biosynthesis and metabolism
类固醇激素生物合成和代谢的免疫组织化学分析
- 批准号:
05670015 - 财政年份:1993
- 资助金额:
$ 1.6万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
Immunocytochemical Study on the Mechanism of Biosynthesis and Secretion of Steroid Hormones
类固醇激素生物合成与分泌机制的免疫细胞化学研究
- 批准号:
01570011 - 财政年份:1989
- 资助金额:
$ 1.6万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
相似海外基金
The role of ZNRF3/Znrf3 exon2 deletion on zonation and maintenance of the adrenal cortex
ZNRF3/Znrf3 外显子 2 缺失对肾上腺皮质分区和维持的作用
- 批准号:
22K08680 - 财政年份:2022
- 资助金额:
$ 1.6万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Investigation of the role of proinsulin-positive bone marrow-derived cells appearing in the adrenal cortex
研究肾上腺皮质中出现的胰岛素原阳性骨髓来源细胞的作用
- 批准号:
21K07338 - 财政年份:2021
- 资助金额:
$ 1.6万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The contribution of capsular and subcapsular progenitor cells in homeostatic adrenal cortex self-renewal and zonal-specific remodelling.
被膜和被膜下祖细胞在稳态肾上腺皮质自我更新和区域特异性重塑中的贡献。
- 批准号:
BB/V007246/1 - 财政年份:2021
- 资助金额:
$ 1.6万 - 项目类别:
Research Grant
Elucidating and modelling complex postnatal steroid hormone changes in preterm infants: insights into the developing immature neonatal adrenal cortex
阐明和模拟早产儿复杂的产后类固醇激素变化:深入了解发育中的未成熟新生儿肾上腺皮质
- 批准号:
MR/V037757/1 - 财政年份:2021
- 资助金额:
$ 1.6万 - 项目类别:
Research Grant
Role of the BMP system and effect on blood pressure regulation in the adrenal cortex
BMP 系统的作用及其对肾上腺皮质血压调节的影响
- 批准号:
20K17512 - 财政年份:2020
- 资助金额:
$ 1.6万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
Elucidation of the mechanism establishing sex difference in adrenal cortex cell proliferation by sex hormones
阐明性激素在肾上腺皮质细胞增殖中建立性别差异的机制
- 批准号:
20K08863 - 财政年份:2020
- 资助金额:
$ 1.6万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Regulation of sexually dimorphic functions of adrenal cortex by sex chromosomes
性染色体对肾上腺皮质性二态性功能的调节
- 批准号:
20H03436 - 财政年份:2020
- 资助金额:
$ 1.6万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Identifying a role of delta-5 steroids from adrenal cortex for development of adrenal medulla
确定肾上腺皮质 delta-5 类固醇对肾上腺髓质发育的作用
- 批准号:
25860764 - 财政年份:2013
- 资助金额:
$ 1.6万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
The production mechanism and role of progesterone in female mouse adrenal cortex.
雌性小鼠肾上腺皮质孕酮的产生机制及其作用。
- 批准号:
22591015 - 财政年份:2010
- 资助金额:
$ 1.6万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Molecular regulation of adrenal cortex homeostasis and remodeling
肾上腺皮质稳态和重塑的分子调节
- 批准号:
7897654 - 财政年份:2009
- 资助金额:
$ 1.6万 - 项目类别: