Cytochemical study on the mechanisms of expression and function of steroid hormone synthesizing enzymes.

(1)研究了17 α -羟化酶/C17-20裂解酶转录因子PbX1在大鼠性腺、肾上腺皮质和胎盘中的表达。只有在胎盘中，转录因子和酶同时表达。(2) 17 α -羟化酶/G17-20裂解酶在1 ~ 3周龄大鼠肝细胞中有短暂表达。这种酶在肝脏中的表达可能与青春期的开始有关。(3)过表达12-脂氧合酶的角质形成细胞出现细胞核结构改变。这种酶可能与角化有关。采用单链DNA抗体免疫组化和原位脱氧核苷酸转移酶染色法检测大鼠睾丸凋亡细胞。结果提示胎儿间质细胞在围产期发生凋亡。NADPH-diaphorase和血管活性肠肽共定位于阴茎海绵状组织。(4)前列腺素F合酶免疫组织化学定位于大鼠中枢神经系统神经细胞的树突。前列腺素F合酶免疫组化定位于大鼠各组织血管内皮细胞。12-脂氧合酶免疫组织化学定位于小鼠胃肠道含血清素的肠内分泌细胞。这种酶可能参与激素的释放。

(1) Expression of PbX1, a transcription factor of 17 alpha-hydroxylase/C17-20 lyase, was studied in the rat gonads, adrenal cortex and placenta. Only in the placenta, both the transcription factor and the enzyme were co-expressed.(2) 17alpha-Hydroxylase/G17-20 lyase was transiently expressed in the hepatocytes of 1 to 3 week-old rats. The expression of the enzyme in the liver may be involved in the onset of puberty.(3) Keratinocytes overexpressing 12-lipoxygenase enzyme showed structural changes of the cell nuclei. The enzyme may relate to keratinization.Apoptotic cells in the rat testis were examined by immunohistochemistry with an antibody against single-stranded DNA and in situ terminal deoxynucleotidyl transferase staining.The results suggested that fetal Leydig cells undergo apoptosis during the perinatal period. NADPH-diaphorase and vasoactive intestinal peptide were colocalized in the penile cavernous tissues.(4) Prostaglandin F synthase was immunohistochemically localized in the dendrites of nerve cells in the rat central nervous system.Prostaglandin F synthase was immunohistochemically localized in the vascular endothelial cells of the rat various tissues.12-Lipoxygenase was immunohistochemically localized in the serotonin-containing entero-endocrine cells of the mouse gastrointestinal tract. The enzyme may be involved in the hormone release.