Cytochemical study on the mechanisms of expression and function of steroid hormone synthesizing enzymes.

类固醇激素合成酶表达和功能机制的细胞化学研究。

• 批准号: 09670016
• 负责人: ISHIMURA Kazunori
• 金额: $ 1.15万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670016/
• 关键词: l7alpha-Hydroxylase; C17-20 1yase; Steroid hormones; Transcription factor; Immunohistochemistry; Electron microscope; Testis; Liver; Placenta; (in press); アポトーシス; プロスタグランジン; PbX1

(1) Expression of PbX1, a transcription factor of 17 alpha-hydroxylase/C17-20 lyase, was studied in the rat gonads, adrenal cortex and placenta. Only in the placenta, both the transcription factor and the enzyme were co-expressed.(2) 17alpha-Hydroxylase/G17-20 lyase was transiently expressed in the hepatocytes of 1 to 3 week-old rats. The expression of the enzyme in the liver may be involved in the onset of puberty.(3) Keratinocytes overexpressing 12-lipoxygenase enzyme showed structural changes of the cell nuclei. The enzyme may relate to keratinization.Apoptotic cells in the rat testis were examined by immunohistochemistry with an antibody against single-stranded DNA and in situ terminal deoxynucleotidyl transferase staining.The results suggested that fetal Leydig cells undergo apoptosis during the perinatal period. NADPH-diaphorase and vasoactive intestinal peptide were colocalized in the penile cavernous tissues.(4) Prostaglandin F synthase was immunohistochemically localized in the dendrites of nerve cells in the rat central nervous system.Prostaglandin F synthase was immunohistochemically localized in the vascular endothelial cells of the rat various tissues.12-Lipoxygenase was immunohistochemically localized in the serotonin-containing entero-endocrine cells of the mouse gastrointestinal tract. The enzyme may be involved in the hormone release.

(1)研究了17 α-羟化酶/C17-20裂解酶的转录因子PbX 1在大鼠性腺、肾上腺皮质和胎盘中的表达。只有在胎盘中，转录因子和酶共同表达。(2)17 α-羟化酶/G17-20裂解酶在1至3周龄大鼠的肝细胞中瞬时表达。这种酶在肝脏中的表达可能与青春期的开始有关。(3)过度表达12-脂氧合酶的角质形成细胞的细胞核结构的变化。应用抗单链DNA抗体免疫组织化学和原位末端脱氧核苷酸转移酶染色法对大鼠睾丸组织中的凋亡细胞进行了检测，结果表明，胎儿睾丸间质细胞在围产期发生了凋亡。NADPH-黄递酶和血管活性肠肽共定位于阴茎海绵体组织。(4)前列腺素F合成酶在大鼠中枢神经系统的神经细胞树突中有化学定位，前列腺素F合成酶在大鼠各种组织的血管内皮细胞中有化学定位，12-脂氧合酶在小鼠胃肠道含降钙素的肠内分泌细胞中有化学定位。这种酶可能参与激素的释放。

项目成果

H.Yokoi: "Steroid 5α-reductase type1immunolocalized in the rar peripheral nervous system and paraganglia." Histochemical Journal. 30. 731-739 (1998)

H. Yokoi：“类固醇 5α-还原酶 1 型免疫定位于 rar 周围神经系统和副神经节。” 组织化学杂志 30. 731-739 (1998)

H.Yokoi: "Leydig Cells Undergoing Apoptosis in the Perinatal Rat Testis." Acta Histochem.Cytochem.31. 355-361 (1998)

H.Yokoi：“围产期大鼠睾丸中的间质细胞发生凋亡。”

H.Yokoi: "Steroid 5α-reductase type 1 immunolocalized in the rat peripheral nervous system and paraganglia." Histochemical Journal. 30. 731-739 (1998)

H. Yokoi：“大鼠周围神经系统和副神经节中的 1 型类固醇 5α-还原酶”，《组织化学杂志》30. 731-739 (1998)。

Nakamura M, Yamamoto S, Ishimura K: "Subcelluar localization of arachidonate 12-lipoxy- genase and morphological effect of itsoverexpression on murine keratinocytes" Cell Tissue Res. 288. 327-334 (1997)

Nakamura M、Yamamoto S、Ishimura K：“花生四烯酸 12-脂氧合酶的亚细胞定位及其过表达对小鼠角质形成细胞的形态学影响”细胞组织研究。

Ishimura K etal: "Light and electron microscopic immunohistochemistry of the localization of adrenal steroidogenic enzymes." Microscopy Research and Technique. 36. 445-453 (1997)

Ishimura K 等人：“肾上腺类固醇生成酶定位的光和电子显微镜免疫组织化学。”