Cytochemical study on the mechanisms of expression and function of steroid hormone synthesizing enzymes.

(1)研究了17α-羟基酶/C17-20裂解酶转录因子Pbx1在大鼠性腺、肾上腺皮质和胎盘中的表达。仅在胎盘中，转录因子和酶同时表达。(2)17α-羟基酶/G17-20裂解酶在1~3周龄大鼠肝细胞中瞬时表达。该酶在肝脏中的表达可能参与了青春期的开始。(3)角质形成细胞过度表达12-脂氧合酶，表现为细胞核结构的改变。用抗单链DNA抗体免疫组织化学和原位末端脱氧核苷酸转移酶染色对大鼠睾丸中的凋亡细胞进行了检测。结果表明，胎儿睾丸间质细胞在围产期发生了凋亡。NADPH-黄递酶和血管活性肠肽共同定位于阴茎海绵体组织。(4)前列腺素F合成酶定位于大鼠中枢神经系统神经细胞树突。前列腺素F合成酶免疫组织化学定位于大鼠不同组织的血管内皮细胞。12-脂氧合酶免疫组织化学定位于小鼠胃肠道内含5-羟色胺的内分泌细胞。这种酶可能参与了激素的释放。

(1) Expression of PbX1, a transcription factor of 17 alpha-hydroxylase/C17-20 lyase, was studied in the rat gonads, adrenal cortex and placenta. Only in the placenta, both the transcription factor and the enzyme were co-expressed.(2) 17alpha-Hydroxylase/G17-20 lyase was transiently expressed in the hepatocytes of 1 to 3 week-old rats. The expression of the enzyme in the liver may be involved in the onset of puberty.(3) Keratinocytes overexpressing 12-lipoxygenase enzyme showed structural changes of the cell nuclei. The enzyme may relate to keratinization.Apoptotic cells in the rat testis were examined by immunohistochemistry with an antibody against single-stranded DNA and in situ terminal deoxynucleotidyl transferase staining.The results suggested that fetal Leydig cells undergo apoptosis during the perinatal period. NADPH-diaphorase and vasoactive intestinal peptide were colocalized in the penile cavernous tissues.(4) Prostaglandin F synthase was immunohistochemically localized in the dendrites of nerve cells in the rat central nervous system.Prostaglandin F synthase was immunohistochemically localized in the vascular endothelial cells of the rat various tissues.12-Lipoxygenase was immunohistochemically localized in the serotonin-containing entero-endocrine cells of the mouse gastrointestinal tract. The enzyme may be involved in the hormone release.