Effects of the beta-adrenergic receptor gene polymorphisms on porcine subcutaneous fat layer thickness

β-肾上腺素受体基因多态性对猪皮下脂肪层厚度的影响

• 批准号: 17580250
• 负责人: CHIKUNI Koichi
• 金额: $ 1.22万
• 依托单位: National Agriculture and Food Research Organization, National Institute of Livestock and Grassland Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17580250/
• 关键词: subcutaneous fat layer; polymorphism; adrenergic receptor; 脂肪; 遺伝子

The full amino acid coding sequences of adrenergic receptor beta1, beta2, and beta3 (ADRB1, ADRB2, and ADRB3) genes were determined from Meishan, Duroc, and Landrace pigs. Non-synonymous substitution of Arg458Pro was found in the pig ADRB1 gene coding a 469 amino acids sequence. Continuous substitutions of Asn29Asp and Glu30Gln were found in the pig ADRB2 gene coding a 418 amino acid sequence. There were three non-synonymous substitutions of Glu24Thr, Arg264Glu and Glu398Asp in the pig ADRB3 gene. A thymine insertion in ADRB3 gene resulting in two amino acids shorter protein was found in Meishan pigs.To assess the effects of ADRB variants on porcine subcutaneous fat layer thickness, genetic frequency of the variants were calculated in fatty and lean groups, consisted of each 24 animals of the crossbred of Duroc and Jinhua pigs. There was no sufficient variation in the ADRB3 gene, and x^2 test showed no significant difference in the ADRB1 and ADBR2 genes of the two groups. However, Arg458 homozygotes of ADRB1 were higher in lean group and had a little thin fat layer than Pro458 variant. The Asp29-Lys30 genotypes of ADRB2 were higher in lean group, and theAsn29-Glu30 genotypes were higher in fatty group. These results suggested that ADRB polymorphism is a minor factor for porcine subcutaneous fat layer thickness.

测定了梅山猪、杜洛克猪和长白猪肾上腺素能受体β 1、β 2和β 3（ADRB 1、ADRB 2和ADRB 3）基因的全氨基酸编码序列。猪ADRB 1基因编码469个氨基酸，存在Arg 458 Pro的非同义替换。猪ADRB 2基因编码418个氨基酸，其中Asn 29 Asp和Glu 30 Gln发生连续替换。猪ADRB 3基因存在Glu 24 Thr、Arg 264 Glu和Glu 398 Asp三个非同义替换。梅山猪ADRB 3基因插入一个胸腺嘧啶，导致编码的蛋白质短了2个氨基酸，为研究ADRB 3基因变异对猪皮下脂肪层厚度的影响，对杜洛克猪与金华猪杂交后代的肥育组和瘦肉组各24头猪进行了ADRB 3基因变异频率的测定。ADRB 3基因变异不充分，经x^2检验，两组ADRB 1、ADBR 2基因差异无统计学意义。而ADRB 1的Arg 458纯合子在瘦肉型中的脂肪含量高于Pro458变异型，且脂肪层较薄。ADRB 2基因Asp 29-Lys 30基因型在瘦肉组中较高，Asn 29-Glu 30基因型在肥胖组中较高。这些结果表明，ADRB基因多态性是影响猪皮下脂肪层厚度的次要因素。