Molecular mechanism of genetic DNA rearrangements in fission yeast

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We are very much interested in molecular mechanism of genetic DNA rearrangements(e. g. homologous recombination), and thus during this proposed research period we tried to elucidate some functions of proteins involved in genetic rearrangements in fission yeast, which was used as a model biological system. Among several approaches we took for this aim, we had a great achievement for analysis of Dmc1 recombinase that works for meiotic recombination. We demonstrated fission yeast Dmc1-mediated Holliday junction formation in vitro. The directionality is 5'-3', which is the same as that by bacterial RecA but the opposite by Rad51 recombinases. Our finding is very important to understand for molecular mechanisms of meiotic recombination that accompanies high level of crossover products.