Molecular mechanism of genetic DNA rearrangements in fission yeast

裂殖酵母遗传DNA重排的分子机制

• 批准号: 21247027
• 负责人: IWASAKI Hiroshi
• 金额: $ 29.29万
• 依托单位: Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21247027/
• 关键词: 相同組換え; DNA二重鎖切断; DNA修復; DNA鎖交換反応; 分裂酵母; Rad22; MRN複合体; Ctp1; Dmc1

We are very much interested in molecular mechanism of genetic DNA rearrangements(e. g. homologous recombination), and thus during this proposed research period we tried to elucidate some functions of proteins involved in genetic rearrangements in fission yeast, which was used as a model biological system. Among several approaches we took for this aim, we had a great achievement for analysis of Dmc1 recombinase that works for meiotic recombination. We demonstrated fission yeast Dmc1-mediated Holliday junction formation in vitro. The directionality is 5'-3', which is the same as that by bacterial RecA but the opposite by Rad51 recombinases. Our finding is very important to understand for molecular mechanisms of meiotic recombination that accompanies high level of crossover products.

我们对遗传DNA重排的分子机制非常感兴趣（例如，G.同源重组），因此在这一拟议的研究期间，我们试图阐明参与裂殖酵母中遗传重排的蛋白质的某些功能，该裂殖酵母被用作模式生物系统。在我们为此目的所采取的几种方法中，我们在分析Dmc 1重组酶方面取得了很大的成就。我们证明了裂殖酵母Dmc 1介导的Holliday连接在体外形成。其方向性为5 '-3'，与细菌RecA相同，而Rad 51重组酶相反。我们的发现对于理解伴随高水平交换产物的减数分裂重组的分子机制非常重要。

项目成果

Regulation of strand exchange reactions by mediator proteins

介导蛋白对链交换反应的调节

• 发表时间: 2009
• 作者: 松永藤彦;石野良純;Hiroshi Iwasaki;M. Hayashi;小林武彦;Hiroshi Iwasaki
• 通讯作者: Hiroshi Iwasaki

Human CtIP and its homolog : Team players in DSB resection games. In DNA repair. On the pathways to Fixing DNA Damage and Errors

人类 CtIP 及其同源物：DSB 切除游戏中的团队成员。

• 发表时间: 2011
• 作者: Tsutsui Y;Kawasaki A and Iwasaki H.
• 通讯作者: Kawasaki A and Iwasaki H.

BIOCHEMICAL ANALYSIS OF FISSION YEAST RECOMBINASES AND THEIR AUXILIARY PROTEINS

裂殖酵母重组及其辅助蛋白的生化分析

• 发表时间: 2009
• 作者: 松永藤彦;石野良純;Hiroshi Iwasaki
• 通讯作者: Hiroshi Iwasaki

Molecular mechanism of DNA strand exchange reaction, a central reaction of homologous recombination mediated by recombinases

DNA链交换反应的分子机制，这是重组酶介导的同源重组的核心反应

• 发表时间: 2011
• 作者: 池田幸樹;八木美江子;森田鉄兵;金井昭夫;饗場弘二;Iwasaki H.
• 通讯作者: Iwasaki H.

RAD6-RAD18-RAD5 pathway-dependent tolerance to chronic low-dose UV light

RAD6-RAD18-RAD5 通路依赖性对慢性低剂量紫外线的耐受性

• 发表时间: 2009
• 期刊: Nature 457
• 作者: Hishida T;Kubota Y;Carr AM and Iwasaki H
• 通讯作者: Carr AM and Iwasaki H