Experimental study to facilitate the repaair of brain injury by artificial modification of cellular function and brain transplantation.

通过细胞功能人工修饰和脑移植促进脑损伤修复的实验研究。

• 批准号: 62570658
• 负责人: HAYAKAWA Toru
• 金额: $ 1.22万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570658/
• 关键词: Cerebral Ischemia; Cerebral Infarction; Cerebral Stab Injury; Cerebral Injury; Cell Proliferation; Mongolian Gerbil; 砂ネズミ; 海馬; 脳組織修復; アストロサイト; ブロモデオキシウリジン; 脳刺創; 脳室上衣下層

The repair process of injury after focal ischemia was studied with special reference on the proliferation of glial population. Therapeutic measure was then searched for to ameliorate or repair the ischemic brain injury.1. The dynamic profile of proliferation of glial cells was studied 1 to 7 days after focal cerebral ischemia in gerbils. The results indicated that the astrocytes proliferated actively at the periphery of infarcted brain during days 1 to 5 postinfarction with most active DNA synthesis at day 3. Additionally, those regions were infiltrated with large number of polymorphonuclear cells and macrophages. The latter type of cells seems especially important since they were positive for interleukin 1 (IL-1).2. Effect of preceding small stab wound on the evolution of hippocampal neuronal death was studied in gerbils. The results indicated that a certain kind of protective mechanism was induced by this type of minor injury to save hippocampal pyramidal neurons from delayed death after transient ischemia. The effect was most impressive when the hippocampus was stabbed 24 hours before ischemic insult.3. The growth and differentiation of transplanted neonatal subependymal cells was studied after transplantation into adult hippocampus. Those cells proliferated for a few weeks in a limited fashion, and differentiated to glial cells but not neurons. We further studied transplantation of fetal subependymal cells, a substantial number of which differentiated to neurons.4. The neonatal hippocampal tissue was transplanted into the hippocampus of adult gerbils at 1 to 4 weeks after transient forebrain ischemia. The results demonstrated that the infarcted hippocampus, especially 1 week after transient ischemia, was feasible for survival of transplanted neural tissue.

研究了局灶性脑缺血后损伤的修复过程，特别是胶质细胞群的增殖。寻找改善或修复缺血性脑损伤的治疗措施.本文观察了沙土鼠局灶性脑缺血后1 ~ 7天胶质细胞增殖的动态变化。结果表明，梗死后1 ~ 5天，梗死灶周围星形胶质细胞增殖活跃，3天DNA合成最活跃。此外，这些区域被大量的多形核细胞和巨噬细胞浸润。后一种类型的细胞似乎特别重要，因为它们对白细胞介素1（IL-1）呈阳性。本实验研究了沙土鼠小刺伤对海马神经元死亡演变的影响。结果提示，这种轻微损伤诱导了一种保护机制，使海马锥体神经元免于短暂缺血后的迟发性死亡。在缺血性损伤前24小时刺入海马，其效果最为显著。将新生大鼠室管膜下细胞移植到成年大鼠海马，观察其生长分化情况。这些细胞以有限的方式增殖了几周，并分化为神经胶质细胞，但不是神经元。我们进一步研究了胚胎室管膜下细胞的移植，其中相当数量的细胞分化为神经元。将新生海马组织移植到成年沙土鼠短暂性前脑缺血后1 ~ 4周的海马内。结果表明，梗死海马，特别是短暂缺血后1周，是移植神经组织存活的可行条件。

项目成果

Yoshimine,T.: J Cerebr Blood Flow Metabol. 7. 387-393 (1987)

Yoshimine,T.：《脑血流代谢杂志》。

Matsumoto, K: "Regional differences in hinhibition and recovery of protein synthesis after transient hindbrain ischemia of gerbils." Neurol Res. 10. 629-635 (1988)

Matsumoto, K：“沙鼠短暂后脑缺血后蛋白质合成的抑制和恢复存在区域差异。”

Sakaguchi, T: "Disturbance in nucleic acid signal transport during delayed neuronal death." Brain Nerve (Tokyo). 40. 629-635 (1988)

Sakaguchi, T：“延迟性神经元死亡过程中核酸信号传输的干扰。”

Toshiki Yoshimine: Journal of Cerebral Blood Flow and Metabolism. 7. 387-393 (1987)

Toshiki Yoshimine：脑血流和代谢杂志。

山田和雄: 豊倉康夫編, 文部省特定研究:神経難病の発症機構11. II. 501-507 (1987)

Kazuo Yamada：Yasuo Toyokura（编辑），教育部特别研究：难治性神经系统疾病的发病机制 11. II. 501-507 (1987)