Immunocytochemical analysis on localization of plant hormones in callus related to regeneration capaci

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To clear the relation between regeneration capacity and localization of plant hormones in callus, a culture system of adventitious shoot formation and embryogenesis from cell suspension of rice (Oryza sativa L.) was established and immunocytochemistry was applied.When rice callus was transferred onto a regeneration medium containing low level of kinetin and ABA, it differentiated embryoid in high rate. On the other hand, when it was transferred onto a medium containing high level of 1-(2-chloro-4-pyridyl)-3-phenylurea (a kind of cytokinin) instead of kinetin, it differentiated adventitious shoots in high rate. Then, it is clear that regeneration pathway can be controlled by culture conditions.To detect IAA and ABA localization, immunocytochemistry was applied to the rice culture system using anti-IAA or anti-ABA monoclonal antibody. Neither IAA nor ABA could be detected, because epitope sites considered to be broken by fixation treatment.