Identification, classification, and information processing in monkey hippocampal neurons in response to loci, objects, and events.

在该项目中，研究了清醒猴子在辨别地点、物体和事件时的海马神经元活动（研究1），以及局限于猴子海马的缺血性神经元死亡对记忆的影响（研究2）。在研究1中，共记录了2169个神经元从海马结构和周围的海马旁回清醒的猴子，而猴子区分各种熟悉的奖励和厌恶的对象以及不熟悉的对象（范例1; n = 1911），而猴子主动控制驾驶室改变其位置内的2.5x2.5米的区域（范例2; n = 258）。在范例1中，视觉反应神经元（n = 160）被分为以下几类，基于他们的生物意义的对象的反应;奖励对象占主导地位，厌恶对象占主导地位，和陌生对象占主导地位的神经元。在范例2中，165个（64.0%）的活动改变了对方向相关刺激或驾驶室控制器位置的反应。 关于我们 或呈现物体（食物或非食物）。在165个有反应的神经元中，有41个对来自特定方向的特定刺激有反应（方向相关）。这些神经元的反应可以用自我中心坐标或非自我中心坐标或这些坐标的组合来描述。79个神经元自发活动的增加或减少取决于动物在2.5x2.5 m视野内的具体位置（位置相关）。这119个神经元在任务的一个或多个阶段中做出反应，这些阶段涉及线索信号，通过握住横杆移动，或者在打开快门时呈现奖励（与任务相关）。这些神经元中的一些表现出特定的组合方向，位置相关，和任务相关的responses.In研究2，猴子进行10 - 15分钟闭塞8（双侧颈总动脉，颈动脉内外，椎）主要动脉缺血性细胞的变化仅限于海马CA1子域。这些患有缺血性损伤的猴子在延迟的不匹配样本测试中显示出显著的记忆障碍，而在运动技能和简单的视觉辨别任务中没有明显的缺陷。静脉输注的钙阻滞剂（NC-1100）显着改善缺血性细胞的变化在猴海马。少

In this project, hippocampal neuronal activity of awake monkey during discrimination of loci, objects, and events (Study 1), and effects of ischemic neuronal death confined to the monkey hippocarnpus on memory (Study 2) were investigated. In Study 1, a total of 2169 neurons were recorded from the hippocampal formation and surrounding parahippocampal gyrus of awake monkeys while the monkey discriminated various familiar rewarding and aversive objects as well as unfamiliar objects (paradigm 1 ; n=1911), and while the monkey actively controlled the cab to change its location within a 2.5x2.5 m region (paradigm 2 ; n=258). In paradigm 1, visually responsive neurons (n=160) were categorized into the following, based on their responsiveness to biological significance of the objects ; rewarding-object-dominant, aversive-object-dominant, and unfamiliar-object-dominant neurons. In paradigm 2, activity of 165 (64.0%) changed in response to direction related stimuli, or location of the cab contai … More ning the subject, or presentation of objects (food or nonfood). Of 165 responding neurons, 41 responded to a particular stimulus presented from a specific direction (direction-related). Responses of these neurons could be described in terms of egocentric or allocentric coordinates, or combinations of these coordinates. Increase or decrease of spontaneous activity of 79 neurons depended on the animal's specific location within the 2.5x2.5 m field (place-related). The 119 neurons responded in one or more phase (s) of a task that involved cue signals, moving by holding the bar, or reward presentation upon opening of a shutter (task-related). Some of these neurons showed specific combinations of directional, placerelated, and task-related responses.In Study 2, the monkey subjected to 10-15 min occlusion of 8 (bilateral common carotid, intemal and extemal carotid, and vertebral) major arteries suffered ischemic cell changes restricted to the CA1 subfield of the hippocampus. These monkeys with ischemic insult indicated significant memory disturbance in a delayed non- matching to sample test, while no deficits in motor-skill nor simple visual discrimination task were evident. The intravenous infusion of a calcium blocker (NC-1100) significantly ameliorated ischemic cell changes in the monkey hippocampus. Less