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Identification, classification, and information processing in monkey hippocampal neurons in response to loci, objects, and events.

猴子海马神经元响应位点、物体和事件的识别、分类和信息处理。

基本信息

批准号：
02404023
负责人：
ONO Taketoshi
金额：
$ 3.2万
依托单位：
Toyama Medical and Pharmaceutical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (A)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02404023/
关键词：
Monkey Hippocampus Single neuron activity Memory Place Brain ischemia Ischemic neuronal death CA1 pyramidal cell 虚血性壊死

项目摘要

In this project, hippocampal neuronal activity of awake monkey during discrimination of loci, objects, and events (Study 1), and effects of ischemic neuronal death confined to the monkey hippocarnpus on memory (Study 2) were investigated. In Study 1, a total of 2169 neurons were recorded from the hippocampal formation and surrounding parahippocampal gyrus of awake monkeys while the monkey discriminated various familiar rewarding and aversive objects as well as unfamiliar objects (paradigm 1 ; n=1911), and while the monkey actively controlled the cab to change its location within a 2.5x2.5 m region (paradigm 2 ; n=258). In paradigm 1, visually responsive neurons (n=160) were categorized into the following, based on their responsiveness to biological significance of the objects ; rewarding-object-dominant, aversive-object-dominant, and unfamiliar-object-dominant neurons. In paradigm 2, activity of 165 (64.0%) changed in response to direction related stimuli, or location of the cab contai … More ning the subject, or presentation of objects (food or nonfood). Of 165 responding neurons, 41 responded to a particular stimulus presented from a specific direction (direction-related). Responses of these neurons could be described in terms of egocentric or allocentric coordinates, or combinations of these coordinates. Increase or decrease of spontaneous activity of 79 neurons depended on the animal's specific location within the 2.5x2.5 m field (place-related). The 119 neurons responded in one or more phase (s) of a task that involved cue signals, moving by holding the bar, or reward presentation upon opening of a shutter (task-related). Some of these neurons showed specific combinations of directional, placerelated, and task-related responses.In Study 2, the monkey subjected to 10-15 min occlusion of 8 (bilateral common carotid, intemal and extemal carotid, and vertebral) major arteries suffered ischemic cell changes restricted to the CA1 subfield of the hippocampus. These monkeys with ischemic insult indicated significant memory disturbance in a delayed non- matching to sample test, while no deficits in motor-skill nor simple visual discrimination task were evident. The intravenous infusion of a calcium blocker (NC-1100) significantly ameliorated ischemic cell changes in the monkey hippocampus. Less

本课题研究了清醒猴在辨别位置、物体和事件时的海马神经元活动(研究一)，以及局限于猴海马体的缺血性神经元死亡对记忆的影响(研究二)。在研究1中，清醒猕猴在辨别各种熟悉的奖赏和厌恶物体以及不熟悉的物体时(范式1；n=1911)，在主动控制驾驶室在2.5×2.5米的范围内改变其位置(范式2；n=258)时，从海马结构及其周围的海马区共记录到2169个神经元。在范式1中，视反应神经元(n=160)根据它们对物体的生物学意义的反应被分为以下几类：奖励性对象显性神经元、厌恶客体显性神经元和陌生客体显性神经元。在范式2中，165%(64.0%)的活动随方向相关的刺激或驾驶室…的位置而改变更多地关注主题，或客体(食物或非食物)的呈现。在165个有反应的神经元中，有41个对来自特定方向(与方向相关)的特定刺激做出反应。这些神经元的反应可以用自我中心坐标或分配中心坐标或这些坐标的组合来描述。79个神经元自发活动的增加或减少取决于动物在2.5x2.5米视野内的特定位置(与位置有关)。119个神经元在一个或多个任务的一个或多个阶段(S)做出反应，涉及到暗示信号、握住操纵杆移动或打开快门时的奖励呈现(与任务相关)。其中一些神经元表现出定向、定位相关和任务相关反应的特定组合。在研究2中，猴子阻断8条主要动脉(双侧颈总动脉、颈动脉内外动脉和椎动脉)10-15分钟后，出现局限于海马区CA1区的缺血细胞变化。这些患有缺血性侮辱的猴子在延迟的样本不匹配测试中表现出显著的记忆障碍，而在运动技能和简单的视觉辨别任务中没有明显的缺陷。静脉注射钙阻滞剂(NC-1100)显著改善了猴子海马区缺血细胞的变化。较少