Study on the involvement of platelet in sudden cardiac death

(1)人心肌肌动蛋白在不同病理条件下的变化我们发现，在死于缺血性心肌梗塞的受试者中，肌球蛋白和托罗普宁被频繁地蛋白分解，这与ATPase活性的变化有关。C级心脏病，但未发现肥大的变化。在一名死于缺血性心脏病的冠状动脉受试者中，我们没有发现来自缺血区和非缺血区的肌球蛋白制剂之间有任何显著差异。(2)犬心肌中钙调素的组织化学研究(II-&心脏性猝死病例)用免疫过氧化物酶方法观察了钙调素的定位。钙调蛋白密度在有收缩带、积水改变的区域增加，甚至在无明显组织学改变的区域增加。这被认为是由于钙结合蛋白的移位，钙调蛋白与膜部分一起对钙超载做出反应。这种方法优于传统的方法。常用的肌球蛋白染色、磷钨酸苏木精染色等方法。(3)不同类型外伤、交通事故和他杀死亡青年的心肌变化，以区分内外因素的参与。坠落死亡的受试者，那些经历了广泛复苏和脑死亡状态延长的受试者，显示出无法与缺血等内在原因的损害区分开来的明显损害。我们还发现，钙调蛋白染色在常规组织学方法中检测到的腔是不明显的。(4)急性酒精摄入对血小板聚集的影响

(1)Alteration - of - human myocardiaI actomvsin under various pathoIogicaI conditionsActomyosins were prepared from myocardia of subjects aged 30-50 years. We found that myosin and toroponin were proteolyzed frequently, associated with the changes in ATPase activity, in subjects who died of ische-mi. c heart disease, but did not find the changes in hypertrophy. - We-did not find'any significant difference between actomyosin preparations from ischemic area and those from non-ischemic area in a single coronary subject who died of ischemic heart disease. -(2)Histochemical study of myocardial, -calmodulin in dog myocardial'ischea(ii-& sudden cardiac death casesLocalization of calmodulin was observed by immuno-peroxidase inethod. Density of calmodulin was increased in areas with-contraction band, hydrovic change, -or even in area without significant histological changes. This is thought to be-due to the translocation of a calcium binding protein, calmodulin with membrane fractions in response to calcium overload. This method is superior to. the popular methods such as myoglobib stiini-ng and phospho-tungustic acid hematoxylin staining.(3)Changes in myocardia in various types of traumaMyocardia of young subjects who died of head injuries, traffic accidents and homicide in an effort to discriminate the involvement of intrinsic and extrinsic factors. Subjects diedof fall, those underwent extensive resuscitation and prolonged status of brain death, showed distinct lesions which were not able to be differentiated from the lesions of intrinsic causes such as ischemia. We also found the usuful 1ness of calmodulin staining which detected the chamges insignificant in conventional histological methods.(4)Effects of acute alcohol ingestion on platelet aggregation