Basal studies on the construction of physical map of silkworm genome DNA.

家蚕基因组DNA物理图谱构建的基础研究.

• 批准号: 03304016
• 负责人: FUJII Hiroshi
• 金额: $ 10.24万
• 依托单位: KYUSYU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03304016/
• 关键词: Bomyx mori; Ptth gene on the 22nd linkage group; APBN gene on the 11th linkage group; RAPD and RFLP; telomere (TTAGG)n sequence; alkaline phosphatase gene; fine structure of E gene complex and Nc genes; アルカリ性ホスファターゼ遺伝子; カイコ Bombyx mori; RFLPの遺伝分析

1.A rapid, simple procedure for preparation of DNA was wstablished.2.Fine structures of E complex and Nc were analyzed by determining their nucleotide sequences. E complex was similar to bithorax gene and Nc was similar to anathenapedia gene of Drosophila.2.Using the RFLP of the third intron of prothorathic galnd stimulating hormone (Ptth), Ptth gene was located at 2.3 on the twenty second likage group and the PABN gene was located at 4.0 on the eleventh linkage group by analyzing the RFLP of the fourth intron of PABN.3.Two genes speified the menbrane bound and unbound alkaline phosphatases (mALP and sASP) were clonedfrom the midgut cDNA library and their nucleotide sequences were determined. Analyzing genome DNA as probes (mALP and sALP cDNAs), two genes may be adjacent location.4.Repeated sequence of the telomere consensus sequence of human as a probe was used for cloning of telomere sequence from silkworm genomic DNA library. Several homologous sequences were cloned and their ristriction enzyme maps were constructed. One nucleotide sequence of them was analyzed and the (TTAGG)n sequence homologous to human was found.

1.建立了一种快速、简便的DNA制备方法。2.通过测定E复合物和Nc的核苷酸序列，分析了它们的精细结构。E复合体与果蝇的双胸基因相似，Nc与果蝇的无胸腺基因相似。通过对PABN基因第4内含子的RFLP分析，Ptth基因位于第22连锁群的2.3位，PABN基因位于第11连锁群的4.0位从中肠cDNA文库中克隆了碱性磷酸酶（mALP和sASP），并测定了它们的核苷酸序列。以家蚕基因组DNA为探针（mALP和sALP cDNA）分析，两个基因可能是相邻的位置。4.以人端粒共有序列的重复序列为探针，从家蚕基因组DNA文库中克隆端粒序列。克隆了几个同源序列，并构建了它们的限制性内切酶图谱。对其中一个核苷酸序列进行分析，发现与人同源的（TTAGG）n序列。

项目成果

Okano,K.: "Basic conditions for the drug selection and transient gene expression in the cultured cell line of Bombyx mori." In Vitro.Cell Devel.Biol.,. 28A. 779-781 (1992)

Okano,K.：“家蚕培养细胞系中药物选择和瞬时基因表达的基本条件。”

Shinohara, T.: "Purification of chymotrypsin inhibitors from larval hemolymph of the Silkworm, Bombyx mori" Biosci.Biotech.Biochem.57. 1067-1071 (1993)

Shinohara, T.：“从家蚕幼虫血淋巴中纯化胰凝乳蛋白酶抑制剂”Biosci.Biotech.Biochem.57。

Yukuhiro, K.: "A type of incompatibility between genes and their genetic background inducing decrease in heterozygote viability approximately equal to that of homozygotes, found in a natural population of Drosophila." Heredity. 71. 74-80 (1993)

Yukuhiro, K.：“基因与其遗传背景之间的一种不相容性，导致杂合子活力下降，与纯合子活力下降大致相同，这种现象在果蝇自然群体中发现。”

安倍広明: "遺伝的モザイクを用いたキコ濃核病ウイルス2型非感受性機構の解析" 日蚕雑. 62. 38-44 (1993)

Hiroaki Abe：“利用遗传嵌合分析对 Kikokoukaryonosis 病毒 2 型的不敏感性机制”Nippon Seriho Zas 62. 38-44 (1993)。

Banno,Yutaka: "Cytologicla analysis of chromosomal aberration due to translocation between the 23rd and 25th linkage groups in the silkworm,Bombyx mori." Hereditas. 118. 259-263 (1993)

Banno, Yutaka：“家蚕第 23 和第 25 连锁群之间易位引起的染色体畸变的细胞学分析。”