Basal studies on the construction of physical map of silkworm genome DNA.

1.建立了一种快速、简便的制备DNA的方法。通过对E配合物和Nc的核苷酸序列分析，分析了它们的精细结构。E复合体与果蝇的双胸基因相似，Nc复合体与果蝇的anathenapedia基因相似。利用促胸腺激素（pth）第3个内含子的RFLP，通过分析pth第4个内含子的RFLP， pth基因位于第22个连锁组的2.3，PABN基因位于第11个连锁组的4.0。从中肠cDNA文库中克隆了膜结合碱性磷酸酶和非膜结合碱性磷酸酶的两个基因mALP和sASP，并测定了它们的核苷酸序列。作为探针分析基因组DNA (mALP和sALP cdna)，两个基因可能位于相邻位置。以人端粒一致序列的重复序列为探针，从家蚕基因组DNA文库中克隆端粒序列。克隆了几个同源序列并构建了它们的限制性内切酶图谱。对其中1个核苷酸序列进行分析，发现与人类同源的（TTAGG）n序列。

1.A rapid, simple procedure for preparation of DNA was wstablished.2.Fine structures of E complex and Nc were analyzed by determining their nucleotide sequences. E complex was similar to bithorax gene and Nc was similar to anathenapedia gene of Drosophila.2.Using the RFLP of the third intron of prothorathic galnd stimulating hormone (Ptth), Ptth gene was located at 2.3 on the twenty second likage group and the PABN gene was located at 4.0 on the eleventh linkage group by analyzing the RFLP of the fourth intron of PABN.3.Two genes speified the menbrane bound and unbound alkaline phosphatases (mALP and sASP) were clonedfrom the midgut cDNA library and their nucleotide sequences were determined. Analyzing genome DNA as probes (mALP and sALP cDNAs), two genes may be adjacent location.4.Repeated sequence of the telomere consensus sequence of human as a probe was used for cloning of telomere sequence from silkworm genomic DNA library. Several homologous sequences were cloned and their ristriction enzyme maps were constructed. One nucleotide sequence of them was analyzed and the (TTAGG)n sequence homologous to human was found.