Study on the biological roles of oligosaccharides : approach by analysis and organic synthesis of oligosaccharides.

低聚糖的生物学作用研究：低聚糖的分析和有机合成方法。

• 批准号: 03404012
• 负责人: OGAWA Tomoya
• 金额: $ 18.62万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (A)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03404012/
• 关键词: Oligosaccharide; Glycoconjugate; Carbohydrate Epitope; Proteoglycan; Glycoprotein; Glycolipids; Placental Lactogen; Equine Chorionic Gonadotropin; GPIアンカー; スフィンゴ糖脂質; PL-Im; 絨毛性性腺刺激ホルモン; ガングリオシド; Rcho-1; N-結合型糖鎖; クロボ系糖脂質; 胎盤性ラクトジエン; PMSG /

Oligosaccharides in biological systems are usually conjugated to lipid or proteins. It is as glycoconjugates that are found in the cytoplasm, on cell membranes and in extra-cellular fluids and matrices. The ability of accurately sequence the oligosaccharide units of glycoconjugates has revealed a remarkable complexity and diversity of these molecules. However, despite some observations suggesting important biological roles for these sugar chains, a single common theory has not emerged to explain this diversity. In this research project, we have studied the biological roles of glycoconjugates by various approach including organic synthesis of oligosaccharides (carbohydrate epitopes of proteoglycans, glycosyl phosphatidylinositol, carbohydrate epitopes of glycoprotein, glycolipids), cDNA cloning (rat placental lactogen, equine chorionic gonadotropin) and production of recombinant glycoproteins, analysis of glycolipids (placental gangliosides) which linked to biological phenomena and morphological approach using lectin- or immuno-staining (mouse placental trophoblast-cells, goat testicular cells).

生物系统中的寡核苷酸通常与脂质或蛋白质缀合。它作为糖缀合物存在于细胞质、细胞膜以及细胞外液和基质中。糖缀合物的寡糖单元的精确测序的能力揭示了这些分子的显著复杂性和多样性。然而，尽管一些观察表明这些糖链具有重要的生物学作用，但还没有一个共同的理论来解释这种多样性。在本研究计划中，我们通过各种方法研究了糖复合物的生物学功能，包括寡糖的有机合成（蛋白聚糖的碳水化合物表位、糖基磷脂酰肌醇、糖蛋白的碳水化合物表位、糖脂）、cDNA克隆（大鼠胎盘催乳素，马绒毛膜促性腺激素）和重组糖蛋白的生产，糖脂（胎盘神经节苷脂）分析，其与生物现象和形态学方法相关，使用凝集素或免疫染色（小鼠胎盘滋养层细胞，山羊睾丸细胞）。

项目成果

Tomoo Nukada: "Synthesis of an octasaccharide fragment of highmannose-type glycans of glycoproteins." Carbohydr.Ress,. 228. 157-170 (1992)

Tomoo Nukada：“糖蛋白高甘露糖型聚糖的八糖片段的合成。”

中原 義昭: "オリゴサッカリンの化学合成ーファイトアレキシンエリシタ-についてー" 有機合成化学協会誌. 49. 821-835 (1991)

Yoshiaki Nakahara：“寡糖精的化学合成 - 关于植物抗毒素诱导剂”有机合成化学学会杂志 49. 821-835 (1991)。

S.Nunomura 他: "Experiments directed towards synthesis of complex glycosphingolipids:Ganglio-ganglioside GQ16." Pure & Appl.Chem.,. 66. 2123-2126 (1994)

S. Nunomura 等人：“针对复合糖鞘脂合成的实验：神经节苷脂 GQ16。Pure & Appl.Chem., 66. 2123-2126 (1994)

B.Schmitz,他: "Determination of structural elements of the L2/HNK-1 carbohydrate epitope required for its function." Glycoconjugate,J.,. 11. 345-352 (1994)

B. Schmitz 等人：“确定其功能所需的 L2/HNK-1 碳水化合物表位的结构元件”，J., 11. 345-352 (1994)。

S.Itonori, et al.: "Glycolipid composition of rat placenta : changes in glycosphingolipids associated with pregnancy stage." Biochem.J.Vol.307, (in press). (1994)

S.Itonori 等人：“大鼠胎盘的糖脂成分：与妊娠阶段相关的鞘糖脂的变化。”