Placental function and development of fetal nervous system.

胎盘功能和胎儿神经系统的发育。

• 批准号: 04304024
• 负责人: OGAWA Tomoya
• 金额: $ 12.16万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04304024/
• 关键词: Placental Lactogen; Fetal Brain; Placenta; 20alpha-Hydroxysteroid Dehydrogenase; Recombinant Protein; Prolactin; Prolactin Receptor; Maternal Behavior; 胎生環境; 向神経因子; 胎盤性ラクトジェン(PL); PL受容体; 単クローン抗体; 神経細胞; グリア細胞; 妊娠; 糖タンパク質; 神経系; シナプス形成; 全胚

The placenta plays an essential role in fetal growth and survival, maintenance of the maternal metabolic status during pregnancy by virtue of its multiple functions relating to the transport of nutrients and waste between mother and fetus, modulation of the maternal and fetal immune systems to prevent rejection of each other, and its endocrinological function of maintaining the pregnancy. The main endocrine participation of the placenta is attributed to placental lactogens(PLs). Considering the phenomenon that twice daily surges of prolactin ceased when secretion of PLs started in the rat and administration of prolactin, which is structurally related to the PLs, stimulates maternal behavior the animals, the PLs are proposed to affect the central nervous function in the dam. In the present study, we showed that the mRNA for PL or prolactin receptor expressed in the fetal rat brain during fetal development. We also succeeded to isolate cDNAclone for a novel PL,PL-Im, and produce the recombinant protein. In the course of these studies, monoclonal antibodies which recognize the molecule in the developing fetal brain were obtained. mRNA for 20alpha-hydroxysteroid dehydrogenase expressed in the placenta to protect the fetus from the maternal steroid environment.

胎盘在胎儿生长和存活、妊娠期间母体代谢状态的维持中发挥重要作用，这是由于胎盘具有与母亲和胎儿之间的营养物和废物运输有关的多种功能、调节母体和胎儿免疫系统以防止彼此排斥以及维持妊娠的内分泌功能。胎盘的主要内分泌参与归因于胎盘催乳素（PL）。考虑到当大鼠开始分泌PLs时，催乳素每天两次的激增停止，并且给予催乳素（其在结构上与PLs相关）刺激动物的母性行为的现象，提出PLs影响母体的中枢神经功能。在本研究中，我们发现，PL或催乳素受体的mRNA表达在胎鼠脑发育过程中。我们还成功地分离了一个新的PL的cDNA克隆，PL-Im，并产生了重组蛋白。在这些研究的过程中，获得了识别发育中的胎儿脑中的分子的单克隆抗体。在胎盘中表达的20 α-羟基类固醇脱氢酶的mRNA，以保护胎儿免受母体类固醇环境的影响。

项目成果

M.Ichikawa: "Effect of differential rearing on synapses and soma size in rat medial amygdaloid nucleus." Synapse. 13. 50-56 (1993)

M.Ichikawa：“差异饲养对大鼠内侧杏仁核突触和体细胞大小的影响。”

K.Shiota, et al.: "Structural and functional aspects of placental lactogens(PLs)and ovarian 20alpha-hydroxysteroid dehydrogenase(20alpha-HSD)in the rat." Endocrine J.41. 43-56 (1994)

K.Shiota 等人：“大鼠胎盘催乳素 (PL) 和卵巢 20α-羟基类固醇脱氢酶 (20α-HSD) 的结构和功能。”

Kunio Shiota: "Maternal control of placental lactogen(s) secretion:Evideuce for extra-placental regulation." Proceedings of the 7th Anuual Meeting Japan Society for Basic Reproductive Immunology.

Kunio Shiota：“胎盘催乳素分泌的母体控制：胎盘外调节的证据。”

K.Shiota,他: "3rd Shirakabako Placenta Conference(20α-Hydroxysteroid dehydrogenase in rat placenta.)" Keiseisha,Tokyo., 13 (1992)

K. Shiota 等人：“第三届 Shirakabako 胎盘会议（大鼠胎盘中的 20α-羟基类固醇脱氢酶）”Keiseisha，东京，13（1992 年）

M.Hirosawa et al.: "A cDNA coding a new member of rat placental lactogen, PL-I mosaic(PL-Im)." Endocrine J.41. 387-397 (1994)

M.Hirosawa 等人：“编码大鼠胎盘催乳素新成员 PL-I 嵌合体 (PL-Im) 的 cDNA。”