Development of chitin and chitosan biomaterials

甲壳素、壳聚糖生物材料的开发

• 批准号: 03555190
• 负责人: SHIGEMASA Yoshihiro
• 金额: $ 9.41万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03555190/
• 关键词: Chitin; Chitosan; Sustained Release; Oxytetracycline; Clinical Case; Wound Healing; Enzymatic Hydrolysis; Partially Deacetylated Chitin; 多形核白血球; 医用材料; 成形加工; 微粒子; 生体内充填材; 人工骨; 遊走能; 乳頭管狭窄治療材

We prepared tablets of oxytetracycline・HCl (OTC・HCl) and chitin powder, followed by covering them with various chitin derivatives, and observed the release of OTC・HCl from the tablet in the phosphate buffer solution. The release rate of OTC・HCl decreased when covering agent varied in the order, carboxymethylchitin > dihydroxypropylchitin > 67% deacetylated chitin.The sponge-, cotton-, and flake-type remedies made of chitin, and non-woven fabric of polyester (NWF) composited with chitin were applied to various types of trauma, abscess, surgical tissue defect and herniorrhaphy in 147 clinical cases including 72 dogs, 38 cows, 33 cats, 2 rabbits, one monkey and one horse. In all cases, good healing developed and no side effects were observed.Partially deacetylated chitins (DACs) prepared under the homogeneous conditions were hydrolyzed by lysozyme more rapidly than DACs prepared under the heterogeneous conditions. The highest lysozyme susceptibility was shown by the DAC around 60-70% deacetylation prepared under the heterogeneous conditions.

制备了土霉素·盐酸盐(OTC·HCl)和甲壳素微粉，并用不同的甲壳素衍生物包衣，考察了土霉素·盐酸盐在磷酸盐缓冲溶液中的释放情况。OTC·HCl的释放率依次为羧甲基甲壳素GT、二羟丙基甲壳素GT、脱乙酰甲壳素67%。用甲壳素制成的海绵状、棉片状和片状敷料，以及聚酯/甲壳质复合无纺布，应用于147例不同类型的创伤、脓肿、外科组织缺损和腹股沟修补术，其中狗72例，牛38例，猫33例，兔2例，猴1例，马1例。在均相条件下制备的部分脱乙酰化几丁质(DAC)被溶菌酶降解的速度比在非均相条件下制备的DAC快。在非均相条件下制备的脱乙酰度在60-70%左右的DAC对溶菌酶的敏感性最高。

项目成果

Y.Okamoto: "Application of Polymeric N-Acetyl-D-glucosamine (Chitin) to Veterinary Practice" J.Vet.Med.Sci.55(5). 743-747 (1993)

Y.Okamoto：“聚合 N-乙酰基-D-葡萄糖胺（甲壳素）在兽医实践中的应用”J.Vet.Med.Sci.55(5)。

Y.Okamoto: "Polymeric N-Acetyl-D-glucosamine(Chitin)Induces Histionic Activation in Dogs" J.Vet.Med.Sci.55. 739-742 (1993)

Y.Okamoto：“聚合 N-乙酰基-D-葡萄糖胺（几丁质）诱导狗的组织酸激活”J.Vet.Med.Sci.55。

Y.Shigemasa: "Sustained Release of Oxytetracycline from Chitin Tablet" Polym.J.25. 993-995 (1993)

Y.Shigemasa：“甲壳素片中土霉素的持续释放”Polym.J.25。

R.A.A.Muzzarelli: "Chitin Enzymology" European Chitin Society, 508 (1993)

R.A.A.Muzzarelli：“甲壳素酶学”欧洲甲壳素协会，508（1993）

Y.Shigemasa: "Enzymatic Degradation of Chitins and Partially Deacetylated Chitins" Int.J.Biol.Macromol.16(1). 43-49 (1994)

Y.Shigemasa：“几丁质和部分脱乙酰化几丁质的酶降解”Int.J.Biol.Macromol.16(1)。