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Development of a new apparatus to observe microcirculation chronically and continuously without restriction of behavior

开发一种长期、连续、无行为限制地观察微循环的新仪器

基本信息

批准号：
06558121
负责人：
IMACHI Kou
金额：
$ 5.82万
依托单位：
University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

A new probe to observe microcirculation without use of microscope was developed, which has small size and can be implanted chronically into a body with minimum invasive. The principle of the probe is quite new. A thin living tissue is put directly on a highly integrated charge coupled device (CCD) and lighted up through the tissue with a very weak light source such as a light-emitting diode. The vascular nets in the tissue are projected on the CCD like a contact photogaph, which is sent to a television screen and can be analyzed their motion and function. A 1/2 inch CCD having 250Kpixels was used in this study. The cover glass on the CCD was removed. A fiber optic plate (FOP) with 3mm thick was attached to the CCD surface so as to keep the surface of the FOP and the ceramic case of the CCD at the same level. The CCD including FOP was molded with silicone rubber for the electric insulation.The resolution of the system tested in vitrorevealed several ten micrometers. The probe was implanted into a rabbit for 18 hrs until the electric insulation was broken. The configuration of arterioles and venules having several ten micrometers in diameter and their motions in a subcutaneous tissue could be observed.The following improvement has been done in the term of the project. The system was molded with epoxy resin to improve the electrical insulation. The tapered FOP that could magnify the figure to 3 times without lens system, was used, which made increase the resolution to be able to observe the flow pattern of erythrocyte. To use the 1/3 inch CCD having 400Kpixels was under consideration as the next step, which would greatly improve the resolution.

研制了一种无需显微镜观察微循环的新型探针，该探针体积小，可长期植入体内，创伤小。这种探测器的原理是相当新的。将薄的活体组织直接放置在高度集成的电荷耦合器件（CCD）上，并用非常弱的光源（如发光二极管）透过组织照亮。组织中的血管网像接触式照相机一样投射到CCD上，并发送到电视屏幕上，可以分析它们的运动和功能。本研究中使用了具有250 K像素的1/2英寸CCD。移除CCD上的盖玻片。将3 mm厚的光纤板（FOP）附接到CCD表面，以使FOP的表面和CCD的陶瓷外壳保持在同一水平。CCD和FOP采用硅橡胶绝缘模压而成，系统的分辨率为几十微米。将探针植入家兔体内18小时，直至电绝缘体被破坏。可以观察到直径为几十微米的小动脉和小静脉的形态及其在皮下组织中的运动。该系统是用环氧树脂模塑，以提高电绝缘。采用了无需透镜系统就能将图像放大3倍的锥形FOP，提高了分辨率，能观察到红细胞的流动形态。下一步考虑使用1/3英寸400 K像素的CCD，这将大大提高分辨率。