权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

FUNDAMENTAL STUDY FOR VASCULAR PROSTHESIS WITH AUTOLOGOUS BONE MARROW TRANSPLANTATION FOR CORONARY ARTERY BY PASS GRAFTING

冠状动脉搭桥术自体骨髓移植人工血管的基础研究

基本信息

批准号：
07457260
负责人：
NOISHIKI Yasuharu
金额：
$ 4.93万
依托单位：
Yokohama City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

We developed a new method for acceleration of neointima formation of a synthetic vascular prosthesis. Various attempts have been tried for this purpose, but no effective method was reported. In order to stimulate cell activities for a hybrid type vascular prosthesis, we used bone marrow transplantation technology autologously into a prosthesis wall. Bone marrow contains young, primitive cells with a strong survival potenial which can differentiate into many kind of mesenchimal cells and can produce many kind of cytokines. As an animal experiment, twelve prostheses was implanted into the abdominal aortae of experimental animals and retrieved from 3 days to 3 months after implantation. Twelve non-treated prostheses were used as the controls. Transplanted marrow cells survived and continued hemopoietic activity. They created erythroblastic islands. Transplanted cells with surrounding cells were immunohistochemically bFGF reactive. Numerous capillary blood vessels were present around the areas of hemopoiesis. The luminal surface of the implanted prosthesis was completely lined with a single layr of endothelial cells within 3 weeks. Control prostheses were not endothelialized at 3 months except at anastomotic sites. From these observations, it is concluded that bone marrow transplantation can accelerate neointima formation on synthetic vascular prostheses. This technology would be use ful for tissue engineering on arificial organs in general.

我们开发了一种新的方法来加速人工血管的新生内膜形成。为此目的已经尝试了各种尝试，但没有有效的方法报道。为了刺激混合型人工血管的细胞活性，我们采用骨髓自体移植技术植入人工血管壁。骨髓中含有年轻、原始的细胞，具有很强的生存能力，可以分化为多种间充质细胞，产生多种细胞因子。作为动物实验，将12个假体植入实验动物的腹主动脉中，并在植入后3天至3个月取出。12个未经处理的假体作为对照。移植的骨髓细胞存活并继续造血活动。它们创造了成红细胞岛。移植的细胞与周围的细胞是免疫化学碱性成纤维细胞生长因子反应。造血区周围有大量毛细血管。3周内，植入假体的管腔表面完全衬有单层内皮细胞。对照假体在3个月时除吻合部位外均未内皮化。从这些观察结果可以得出结论，骨髓移植可以加速合成血管假体的新生内膜形成。该技术可广泛应用于人工器官的组织工程。