Fundamental study for autocrine artificial

我们研究了自分泌人工器官。作为一个例子，我们开发了一个汽车人造血管假体。移植物可以合成血管生成生长因子，通过移植物腔面加速内皮细胞的生长。作为假体的框架，我们采用了长纤维长度的e=PTFE移植物，可以在移植物壁内捕获大量的细胞和组织碎片。因为，血管假体需要血管生成来实现管腔表面的内皮化，因为内皮细胞具有天然和永久的抗血栓形成特性。为了诱导毛细血管生长到移植物中，我们开发了一种方法，将原始的、足够强大的骨髓细胞移植到移植物中，并产生血细胞，从而诱导毛细血管生长。在动物实验中，骨髓细胞浸润到长纤维膨胀聚四氟乙烯（e-PTFE）血管移植物的壁中。将移植物自体植入24只犬的腹主动脉位置。骨髓细胞存活并持续外源性造血长达6个月，对碱性成纤维细胞生长因子（bFGF）具有免疫组织化学反应。所有大于3周的移植物均完全内皮化并保持通畅。无骨髓移植20例作为对照。吻合口出现内皮化，但其他部位被新鲜血栓覆盖。7个对照移植物中有4个在6个月时内皮细胞内衬通畅，但3个被阻塞，其中1个移植物仍被血栓层覆盖。骨髓以其独特的天然特性在移植物中产生自分泌血管生成能力。

We investigated autocrine artificial organs. As an example, we developed an aotocrine artificial blood vessel prosthesis. The graft can synthcsize angiogenic growth factors which can accelcrate endothelial cell lining though out thc graft luminal surface. As the framework of the prosthesis, we adopted a long fibril length e=PTFE graft which can trap numerous cells and fissue fragments inside the graft wall. Because, a vascular prosthesis needs angiogenesis for endothelialization of the luminal surface, as endothleeial cells have natura1 and permanent antithrombogenic properties. To induce capillary growth into the graft, we developed a method of transplanting bone marrow cells, which are primitive, strong enough to survive, and create blood cells, resulting in the inducement of capillary growth. In an animal experiment, marrow cells were infiltrated into the walls of long-fibril expanded polytetrafluoroethylene (e-PTFE) vascular grafts. The grafts were implanted in the abdominal aortic position of 24 dogs autologously. Marrow cells survived and continued exogenous hemopoiesis for up to six monthsand were immunohistochemically reactive to basic fibroblast growth factor (bFGF).All the grafts older than three weeks had complete endothelialization and maintained their patency. Twenty grafts without bone marrow were implanted as controls. Endothelialization was present at anastomotic sites, but other areas were covered with fresh thrombi. Four out of seven control grafts were patent with endothelial cells lining at six months, but three were occluded and one of the four grafts was still covered with a thrombus layer. Bone marrow with its unique native propertires produced autocrine angiogenicity in the graft.