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Fundamental study for autocrine artificial

自分泌人工基础研究

基本信息

批准号：
09480256
负责人：
NOISHIKI Yasuharu
金额：
$ 8.9万
依托单位：
Yokohama City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09480256/
关键词：
Vascular prosthesis Neointima basic fibroblast growth factor Hybrid Endothelial cells cell guidance Bone marrow Autocrine artificial prostheses ハイブリッド疎血心筋皮膚移植骨随

项目摘要

We investigated autocrine artificial organs. As an example, we developed an aotocrine artificial blood vessel prosthesis. The graft can synthcsize angiogenic growth factors which can accelcrate endothelial cell lining though out thc graft luminal surface. As the framework of the prosthesis, we adopted a long fibril length e=PTFE graft which can trap numerous cells and fissue fragments inside the graft wall. Because, a vascular prosthesis needs angiogenesis for endothelialization of the luminal surface, as endothleeial cells have natura1 and permanent antithrombogenic properties. To induce capillary growth into the graft, we developed a method of transplanting bone marrow cells, which are primitive, strong enough to survive, and create blood cells, resulting in the inducement of capillary growth. In an animal experiment, marrow cells were infiltrated into the walls of long-fibril expanded polytetrafluoroethylene (e-PTFE) vascular grafts. The grafts were implanted in the abdominal aortic position of 24 dogs autologously. Marrow cells survived and continued exogenous hemopoiesis for up to six monthsand were immunohistochemically reactive to basic fibroblast growth factor (bFGF).All the grafts older than three weeks had complete endothelialization and maintained their patency. Twenty grafts without bone marrow were implanted as controls. Endothelialization was present at anastomotic sites, but other areas were covered with fresh thrombi. Four out of seven control grafts were patent with endothelial cells lining at six months, but three were occluded and one of the four grafts was still covered with a thrombus layer. Bone marrow with its unique native propertires produced autocrine angiogenicity in the graft.

我们研究了自分泌人造器官。作为一个例子，我们开发了一个自分泌人工血管假体。移植物可以吸收血管生成因子，这些因子可以加速内皮细胞在移植物腔表面的衬里。作为假体的框架，我们采用了一种长纤维长度的e = PTFE移植物，它可以将大量的细胞和裂隙碎片捕获在移植物壁内。因为，血管假体需要血管生成来实现管腔表面的内皮化，因为内皮细胞具有天然和永久的抗血栓形成特性。为了诱导毛细血管生长到移植物中，我们开发了一种移植骨髓细胞的方法，骨髓细胞是原始的，足够强壮以存活并产生血细胞，从而诱导毛细血管生长。在动物实验中，骨髓细胞浸润到长纤维膨胀聚四氟乙烯（e-PTFE）血管移植物的壁中。将移植物植入24只犬的腹主动脉位置。骨髓细胞存活并持续外源性造血达6个月，并对碱性成纤维细胞生长因子（bFGF）呈免疫反应性，移植3周以上的移植物均完全内皮化并保持通畅。植入20个不含骨髓的移植物作为对照。在吻合部位存在内皮化，但其他区域被新鲜血栓覆盖。在6个月时，7个对照移植物中有4个是通畅的，内皮细胞衬里，但3个被闭塞，4个移植物中有1个仍然覆盖有血栓层。骨髓以其独特的天然性质在移植物中产生自分泌血管生成。