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FUNDAMENTAL STUDY FOR DEVELOPMENT OF AUTOCRINE ARTIFICIAL ORGANS

自分泌人工器官发育的基础研究

基本信息

批准号：
07558259
负责人：
NOISHIKI Yasuharu
金额：
$ 0.83万
依托单位：
Yokohama City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

We developed a model of autocrine artificial organ of hybrid type, which is a completely new concept in the field of artificial organs. In order to develop a new functional artificial organs, various attempts have been tried using combinational use of synthetic materials and functional living cells. These cells were highly differentiated with a special function. However, in our experiment, we used bone marrow transplantation technology autologously into a prosthesis wall. Bone marrow contains young, primitive cells with a strong survival potential which can differentiate into many kind of mesenchimal cells and can produce many kind of cytokines. Animal experiment showed that transplanted marrow cells created erythroblastic islands. Transplanted cells with surrounding cells were immunohistochemically bFGF reactive. Numerous capillary blood vessels were present around the areas of hemopoiesis. The luminal surface of the implanted prosthesis was completely lined with a single layr of endothelial cells within 3 weeks. Control prostheses were not endothelialized at 3 months except at anastomotic sites. From these observations, it is concluded that bone marrow transplantation can accelerate neointima formation on synthetic vascular prostheses. The transplanted cells produce cytokines and growth factors which stimlate the cells in the artificial organs resulted in rapid neointima formation. A kind of autocrine artificial organ will be possible in this manner.

本文提出了一种杂交型自分泌人工器官模型，这是人工器官领域的一个全新概念。为了开发新的功能性人工器官，已经尝试了使用合成材料和功能性活细胞的组合使用的各种尝试。这些细胞高度分化，具有特殊的功能。然而，在我们的实验中，我们将骨髓移植技术自体移植到假体壁中。骨髓中含有年轻、原始的细胞，具有很强的存活能力，可以分化为多种间充质细胞，产生多种细胞因子。动物实验表明，移植的骨髓细胞可形成成红细胞岛。移植的细胞与周围的细胞是免疫化学碱性成纤维细胞生长因子反应。造血区周围有大量毛细血管。3周内，植入假体的管腔表面完全衬有单层内皮细胞。对照假体在3个月时除吻合部位外均未内皮化。从这些观察结果可以得出结论，骨髓移植可以加速合成血管假体的新生内膜形成。移植的细胞产生细胞因子和生长因子，刺激人工器官中的细胞，导致快速的新生内膜形成。一种自分泌的人工器官将有可能以这种方式。