FUNDAMENTAL STUDY FOR VASCULAR PROSTHESIS THAT CAN ABSORBE ENDOGENOUS CYTOKINES

我们开发了一种利用内源性细胞因子吸收系统加速合成织物人工血管新生内膜形成的新方法。通过这项技术，我们创造了一种血管假体，可以很容易地形成新内膜。我们认为，在人工血管上内皮细胞形成的新生内膜是体内的组织工程产物。为了获得成功的组织工程化新生内膜，我们需要三个主要组成部分，即，合适的细胞、细胞外基质和细胞因子。织物人造血管没有这些组件。在假体植入过程中，许多细胞在手术区域受到损伤。这些细胞合成多种细胞因子和细胞生长因子用于组织修复。因此，如果人造血管能够将它们保持在原位，新生内膜将很好地形成。根据这一假设，我们寻找了一种适合细胞迁移和细胞增殖以及细胞因子吸附的基质。结果表明，疏水性胶原蛋白是最佳基质。通过动物实验，我们证实了大量毛细血管长入涂有特殊胶原蛋白的织物移植物中，导致新生内膜迅速形成。

We developed a new method for acceleration of neointima formation of a synthetic fabric vascular prosthesis using endogenous cytokine absorption system. With this technology, we created a vascular prosthesis that can have a neointima easily. We assumed that neointima formation with endothelial cells onto synthetic vascular prostheses was a tissue engineered product in vivo. In order to obtain a successful tissue engineered neointima, we need three major components, i.e., appropriate cells, extracellular matrix, and cytokines. Fabric vascular prostheses have no these components. During prosthesis implantation, numerous cells are injured at the operation field. These cells synthesize many kinds of cytokines and cell growth factors for tissue repair. Therefore, if a vascular prosthesis can keep them in situ, neointima will be created nicely. With the hypothesis, we searched an appropriate matrix for cell migration and cell proliferation, and cytokine adsorption. With the results using RI raveled growth factors, we found a hydrophobic collagen as the best matrix. With an animal study, we confirmed numerous capillary ingrowth into the fabric grafts coated with the special collagen, resulted in neointima formation rapidly.