FUNDAMENTAL STUDY FOR VASCULAR PROSTHESIS THAT CAN ABSORBE ENDOGENOUS CYTOKINES

吸收内源性细胞因子的血管假体的基础研究

• 批准号: 11470249
• 负责人: NOISHIKI Yasuharu
• 金额: $ 9.6万
• 依托单位: YOKOHAMA CITY UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11470249/
• 关键词: vascular prosthesis; neointima; growth factor; cytokine adsorption; endothelial cells; tissue engineering; hydrophobic collagen; endogenous cytokine; 物理的架橋; グルタールアルデヒド; サイトカイン; 細胞誘導

We developed a new method for acceleration of neointima formation of a synthetic fabric vascular prosthesis using endogenous cytokine absorption system. With this technology, we created a vascular prosthesis that can have a neointima easily. We assumed that neointima formation with endothelial cells onto synthetic vascular prostheses was a tissue engineered product in vivo. In order to obtain a successful tissue engineered neointima, we need three major components, i.e., appropriate cells, extracellular matrix, and cytokines. Fabric vascular prostheses have no these components. During prosthesis implantation, numerous cells are injured at the operation field. These cells synthesize many kinds of cytokines and cell growth factors for tissue repair. Therefore, if a vascular prosthesis can keep them in situ, neointima will be created nicely. With the hypothesis, we searched an appropriate matrix for cell migration and cell proliferation, and cytokine adsorption. With the results using RI raveled growth factors, we found a hydrophobic collagen as the best matrix. With an animal study, we confirmed numerous capillary ingrowth into the fabric grafts coated with the special collagen, resulted in neointima formation rapidly.

我们开发了一种利用内源性细胞因子吸收系统加速人造纤维血管假体新生内膜形成的新方法。利用这项技术，我们创造了一种血管假体，可以很容易地形成新的血管内膜。我们认为人造血管假体与内皮细胞结合形成新生内膜是体内组织工程的产物。为了获得成功的组织工程化新生内膜，我们需要三个主要成分，即合适的细胞、细胞外基质和细胞因子。织物血管假体没有这些成分。假体植入过程中，大量细胞在术野受到损伤。这些细胞合成多种细胞因子和细胞生长因子，用于组织修复。因此，如果血管假体能够将它们保持在原位，就会很好地创造新的内膜。在这个假设下，我们寻找了一种适合细胞迁移和细胞增殖以及细胞因子吸附的基质。结合RI分离生长因子的结果，我们发现疏水性胶原是最好的基质。通过动物研究，我们证实了大量毛细血管生长到涂有特殊胶原蛋白的织物移植物中，导致新的内膜迅速形成。

项目成果

Makoto Ando et al: "Autologous Tissue-Fragmented Extracardiac Conduit With Rapid, Stable Endothelialization due to Angigenesis."J.Thoracic Cardiovascular Surgery,. 48. 153-160 (2000)

Makoto Ando 等人：“自体组织碎片心外导管由于血管生成而具有快速、稳定的内皮化作用。”J.胸心血管外科，。

Yasuharu Noishiki, et al: "Age dependency of Neointima formation on vascular prostheses in dogs."Artificial Organs. 24(9). 718-728 (2000)

Yasuharu Noishiki 等人：“狗血管假体上新内膜形成的年龄依赖性。”人工器官。

Makoto Ando et al: "Autologous Tissue-Fragmented Extracardiac Conduit With Rapid, Stable Endothelialization due to Angigenesis."J.Thoracic Cardiovascular Surgery,. 48(3). 153-160 (2000)

Makoto Ando 等人：“自体组织碎片心外导管由于血管生成而具有快速、稳定的内皮化作用。”J.胸心血管外科，。

Yasuharu Noishiki et al: "Angiogenic growth factor release system for in vivo tissue engineering : a trial of bone marrow transplantation into ischemic myocardium"Journal of Artificial Organs. 2. 85-91 (1999)

Yasuharu Noishiki 等人：“用于体内组织工程的血管生成生长因子释放系统：骨髓移植到缺血心肌的试验”人工器官杂志。

Yasuharu Noishiki: "Bone marrow transplantation in vascular prostheses."Materials Science and Engineerig. 6(4). 227-233 (1998)

Yasuharu Noishiki：“血管假体中的骨髓移植。”材料科学与工程。