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Development of a Culture System of Living Tissues under Dynamic Load Condition

动态负载条件下活组织培养系统的开发

基本信息

批准号：
07558124
负责人：
HAYASHI Kozaburo
金额：
$ 9.22万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07558124/
关键词：
Living Tissue Tissue Culture Dynamic Loading Collagen Fascicle Remodeling Biomechanics

项目摘要

1.To study the function of living tissues cultured under various mechanical loading conditions, a system was designed for the application of dynamic load to cultured tissues, which consists of a tissue culture chamber, a linear actuator, and a load cell. The temperature in the culture chamber is maintained at 37ﾟC with heated water circulation, and pH of the medium is maintained with a mixture of 95% air and 5% CO_2 gas.2.To evaluate the performance of the system, collagen fascicles obtained from a rabbit patellar tendon were cultured under static load condition. The viability of fibroblasts in the fascicles was maintained at the level of non-cultured, control fascicles for 2 weeks.3.The material of culture dish and grips were changed from polymethylmethacrylate to polycarbonate for sterilization in an autoclave.4.Collagen fascicles were cultured under static load condition for 1 and 2 weeks, and their mechanical properties were determined. Tensile strength and tangent modulus of collagen fascicles cultured under static load condition for 2 weeks were similar to those of non-cultured, control fascicles. A significant correlation was observed in cultured collagen fascicles between accumulated applied stress and tensile strength.5.Collagen fascicles were, then, cultured under cyclic load condition for 1 and 2 weeks. The tensile strength and tangent modulus of the fascicles cultured under the cyclic tensile load of 50% of in vivo peak stress were almost the same as those of control fascicles.

1.为了研究在不同机械载荷条件下培养的活组织的功能，设计了一种对培养组织施加动态载荷的系统，该系统由组织培养室、线性执行器和称重传感器组成。培养室温度保持在37ºC，加热水循环，95%的空气和5%的CO_2气体混合，保持培养基pH。为了评估该系统的性能，我们在静载条件下培养了兔髌骨肌腱中的胶原束。成纤维细胞在肌束中的活力维持在未培养的对照肌束的水平2周。将培养皿和握把的材料由聚甲基丙烯酸甲酯改为聚碳酸酯，在高压灭菌器中灭菌。静载条件下培养胶原束1周和2周，测定其力学性能。静载条件下培养2周的胶原束的拉伸强度和切线模量与未培养的对照束相似。在培养的胶原束中观察到累积施加应力与抗拉强度之间的显著相关性。然后在循环负荷条件下培养胶原束1周和2周。在体内峰值应力50%的循环拉伸载荷下培养的肌束的抗拉强度和切线模量与对照肌束基本相同。