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Development of a tensile test system for cells and biological fine fibers

细胞和生物细纤维拉伸测试系统的开发

基本信息

批准号：
09358020
负责人：
HAYASHI Kozaburo
金额：
$ 16.51万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09358020/
关键词：
Biomechanics Cell Collagen fiber Stiffness Tensile tester 生体タンパク分子生体組織力学的性質

项目摘要

A tensile test system for cells and biological fine fibers has been designed, and the tensile properties of fibroblasts, vascular smooth muscle calls, and collagen fibers have been first determined with this system.1.The system consists of an inverted fluorescence microscope, micromanipulator, a direct drive linear actuator, a load cell, a video dimension analyzer (VDA), and a thermostatic test chamber. The test chamber and the microscope are mounted on a vibration isolator. A cell or a fiber is gripped with a pair of micropipettes at their fine tips using a cell adhesive or a polymer adhesive. One of the micropipettes is fixed to the load cell; the other one is attached to the linear actuator which is used to stretch the specimen. The load cell is of a cantilever-type; the deflection of the cantilever is measured with a semi-conductor strain gage or a laser displacement meter, and converted into load (accuracy=±0.05μN). Specimen elongation is determined from the distance between the micropipette tips measured with the VDA (resolution=0.24μm).2.The stiffness, maximum load, and elongation to failure of the fibroblasts harvested from the rabbit patellar tendon and then cultured in vitro (diameter at no load=21μm) were 0.02N/m, 0.9μN, 86μm, respectively, and the cell shape was locally irregular during tensile testing.3.The stiffness of the smooth muscle cells harvested from the rabbit thoracic aorta and then cultured in vitro (diameter at no load=32μm) was 0.09N/m in synthetic-typed cells and 0.17N/m in contractile-typed cells.4.The secant modulus, tensile strength, and strain to failure of the collagen fibers obtained from the rabbit patellar tendon (diameter=1μm) were 54MPa, 8.5MPa, and 22%, respectively.

设计了一套细胞和生物细纤维的拉伸试验系统，并利用该系统首次测定了成纤维细胞、血管平滑肌细胞和胶原纤维的拉伸性能。1.该系统由倒置荧光显微镜、显微操作器、直线驱动器、测力传感器、视频尺寸分析仪（VDA）和拉伸试验箱组成。测试箱和显微镜安装在隔振器上。使用细胞粘合剂或聚合物粘合剂，用一对微量移液器在其精细尖端处夹持细胞或纤维。其中一个微量移液器固定在测力传感器上;另一个连接在用于拉伸样本的线性致动器上。测力传感器为悬臂式，悬臂的挠度用半导体应变计或激光位移计测量，并转换为载荷（精度=±0.05μN）。样品伸长率由VDA测量的微量移液器吸头之间的距离确定。（分辨率=0.24μm）2.体外培养兔髌腱成纤维细胞，测定其刚度、最大载荷和断裂伸长率（空载时直径=21μm）分别为0.02 N/m、0.9μN、86μm，体外培养的兔胸主动脉平滑肌细胞的刚度4.兔髌腱胶原纤维（直径1μm）的割线模量、拉伸强度和断裂应变分别为54 MPa、8.5MPa和22%。