フッ化物の骨髄幹細胞の増殖・分化に及ぼす影響

氟化物对骨髓干细胞增殖和分化的影响

• 批准号: 07771653
• 负责人: 川瀬 知之
• 金额: $ 0.7万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07771653/
• 关键词: フッ化ナトリウム; 活性型ビタミンD_3; HL-60細胞; 骨髄細胞; 分化

最初に、ヒト急性白血病由来全骨芽球細胞株(HL-60)を未分化骨髄細胞株のモデルとして用い、NaFがその細胞の増殖・分化にどのような影響を及ぼすかについて検討した。この細胞は自発的増殖活性を維持しているため、NaF処理時間を最大4日間に限定したうえで、活性型ビタミンD_3(Vit.D_3)の存在・非存在下で実験を行った。ここで、増殖・分化の指標として細胞数、細胞内エステラーゼ活性、NBT還元活性、表面抗原(CD11b,CD66b)の発現度、酸性ホスファターゼ活性、NO産生、サイトカイン(IL-1α,IL-6,TNF-α)産生、PGE_2産生に注目した。その結果、NaF(0.5mM)単独処理でも弱いながらHL-60細胞を顆粒球方向へ分化促進させる作用が認められたが、Vit,D_3と併用することによって、この効果が顕著に増強されることを見い出した。また、その作用機序に関しては、内因性PGE_2の関与が強く示唆された。サイトカインやNOなどによる分化促進作用の可能性もたびたび報告されているが、我々の中和抗体を用いた実験からは有意な結果が得られなかった。以上の所見を総合すると、Vit,D_3とNaFの併用はHL-60細胞をPGE_2産生を介して顆粒球方向へ分化させることが示唆された。現在、この結果を踏まえ実験材料をマウス初代培養骨髄細胞に移して検討中である。Preliminary studyの段階ではあるが、NaF単独処理で顆粒球特異的表面抗原の発現が有意に増加するということは特筆に値するものと思われる。

In the initial stage of acute leukemia, the whole bone germ cell line (HL-60), the undifferentiated bone cell line, the cell line, the colony differentiation cell line, the colony differentiation cell line, the whole bone germ cell line (HL-60), the undifferentiated bone cell line, the whole bone blastocyst cell line, the undifferentiated bone blastocyst cell line, the whole bone blastocyst cell line, the undifferentiated bone blastocyst cell line, the whole bone blastocyst cell line, the undifferentiated bone blastocyst cell line, the whole bone blastocyst cell line, the undifferentiated bone blastocyst cell line, the whole bone bud ball cell line, the undifferentiated bone cell line, the whole bone bud ball cell line, the undifferentiated bone cell line, the whole bone bud ball cell line, the undifferentiated bone cell line, the whole bone bud ball cell line, the undifferentiated bone cell line, the whole bone bud The colonization activity of the cell culture maintained the restriction enzyme activity, the maximum NaF time limit was 4 days, and the active cell cycle D3 (Vit.D_3) was found to be non-existent and non-existent. Colony differentiation refers to the number of cells, the activity of intracellular antigen, the activity of NBT, the degree of surface antigen (CD11b,CD66b), the activity of acid antigen, the activity of NO, the activity of IL-1 α, IL-6,TNF- α, and PGE_2. The results of NaF (0.5mM) show that the differentiation of HL-60 cells in the direction of cell differentiation promotes the differentiation of cells. The results show that the differentiation of cells in the direction of cell differentiation promotes the differentiation of cells in the direction of cell differentiation, the results show that the differentiation of cells in the direction of cell differentiation promotes the differentiation of cells in the direction of cell differentiation, the results show that the differentiation of cells in the direction of cell differentiation promotes the differentiation of cells. The sequence of mechanisms of action of drugs and drugs is to induce diseases, and the internal causes of PGE_2 are abnormal and forced to indicate instigation. The possibility of differentiation was induced by NO. The report reported that the neutralizing antibody was not effective, and that the neutralizing antibody was intentionally detected with the result of intentional detection. As seen above, Vit,D_3 NaF cells use HL-60 cells, PGE_2 cells, particles to differentiate, and PGE_2 to show that they are instigated. Now, the results show that the material is very important in the primary culture of bone cells. The surface antigens of Preliminary study, NaF, and granule specific antigen were detected, and the specific antigen was detected.