Search for methods to control and treat allergy by regulating class switch recombination

寻找通过调节类别转换重组来控制和治疗过敏的方法

• 批准号: 24659147
• 负责人: SHIMIZU Akira
• 金额: $ 2.5万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659147/
• 关键词: 免疫学; クラススイッチ; 遺伝子組換え; アレルギー; 免疫制御

Focused on the regulation of IgE production and regulation of class switching to IgA, a trial to manipulate of the target gene specificity of class switch recombination (CSR) by intervening its place or regulatory factors was performed.It was examined whether the specific CSR could be controlled in an in vitro culture by the factors like Id2 and Runx2/3 which are known to be involved in the regulation of CSR to IgE and IgA, respectively. It was also examined whether such factors could guide the cells to switch to IgA after the cells were induced to IgE CSR.By the above experiments, a possibility was tested that intervening the place or regulatory factors of CSR to manipulate specificity of CSR and could be a target to control allergy by eliminating IgE and to strengthen first-line protection against infectious diseases by enhancing IgA production.

以调节IgE的产生和调节类转换为IgA为中心，尝试通过干预类开关重组(CSR)的位置或调节因子来调控CSR的靶基因特异性，以检验在体外培养条件下，特异性CSR能否分别被参与CSR对IgE和IgA的调节的Id2和Runx2/3等因子调控。通过上述实验，验证了通过干预CSR的位置或调节因子来调控CSR的特异性，并可作为通过清除IgE来控制变态反应和通过增加IgA的产生来加强对传染病的一线保护的目标。

项目成果

In situ differentiation of CD8aa T cells from CD4 Tcells in peripheral lymphoid tissues

外周淋巴组织中 CD8aa T 细胞与 CD4 T 细胞的原位分化

• 发表时间: 2012
• 作者: Nambu Y;Hayashi T;Jang KJ;Aoki K;Mano H;Nakano K;Osato M;Takahashi K;Itoh K;Teramukai S;Komori T;Fujita J;Ito Y;Shimizu A;Sugai M
• 通讯作者: Sugai M

Functional Evaluation of Activation-dependent Alterations in the Sialoglycan Composition of T Cells

• DOI: 10.1074/jbc.m113.523753
• 发表时间: 2014-01-17
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Naito-Matsui, Yuko;Takada, Shuhei;Takematsu, Hiromu
• 通讯作者: Takematsu, Hiromu

Novel Collagen/Gelatin Scaffold with Sustained Release of Basic Fibroblast Growth Factor: Clinical Trial for Chronic Skin Ulcers

• DOI: 10.1089/ten.tea.2012.0634
• 发表时间: 2013-09-01
• 期刊: TISSUE ENGINEERING PART A
• 影响因子: 4.1
• 作者: Morimoto, Naoki;Yoshimura, Kenichi;Suzuki, Shigehiko
• 通讯作者: Suzuki, Shigehiko

Evasion from accelerated blood clearance of nanocarrier named as "Lactosome" induced by excessive administration of Lactosome

• DOI: 10.1016/j.bbagen.2013.03.024
• 发表时间: 2013-08-01
• 期刊: BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
• 影响因子: 3
• 作者: Hara, Eri;Makino, Akira;Kimura, Shunsaku
• 通讯作者: Kimura, Shunsaku

An exploratory clinical study on the safety and efficacy of an autologous fibroblast-seeded artificial skin cultured with animal product-free medium in patients with diabetic foot ulcers

• DOI: 10.1111/j.1742-481x.2012.01064.x
• 发表时间: 2014-04-01
• 期刊: INTERNATIONAL WOUND JOURNAL
• 影响因子: 3.1
• 作者: Morimoto, Naoki;Ito, Tatsuya;Suzuki, Shigehiko
• 通讯作者: Suzuki, Shigehiko