权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Xenotransplantation from GalT-KO miniture swine to baboon in tolerance induction protocol

在耐受诱导方案中从 GalT-KO 小型猪到狒狒的异种移植

基本信息

批准号：
20591900
负责人：
SHIMIZU Akira
金额：
$ 3万
依托单位：
Nippon Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2008
资助国家：
日本
起止时间：
2008 至 2010
项目状态：
已结题

项目摘要

Xenotransplantation could provide a solution to the critical shortage of organs for transplantation in humans. In attempt to prevent hyperacute rejection (HAR) and acute humoral xenograft rejection (AHXR), α1,3-galactosyltransferase gene knockout (GalT-KO) pigs have been produced in Massachusetts General Hospital, Boston, USA. In heart xenotransplantation from GalT-KO pig into baboon, heterotopic heart grafts could beat in abdominal cavity by 179 days (median 78 days). In kidney xenotransplantation, GalT-KO kidney grafts rejected by day 34 in chronic immunosuppression protocol, but grafts could survive up to 83 days in tolerance inducing protocol. GalT-KO heart and kidney xenografts did not develop hyperacute rejection. However, all GalT-KO heart and kidney grafts in the chronic immunosuppression group were rejected by AHXR with thrombotic microangiopathy. Acute cellular xenograft rejection (ACXR) and chronic rejection also developed. However, in the kidney grafts in tolerance inducing protocol, AHRX, ACXR, and chronic rejection could not be detected in the grafts. Therefore, we conclude that the tolerance inducing protocol is necessary for success the heart and kidney xenotransplantation from GalT-KO pig into baboons.

异种移植可以解决人类移植器官严重短缺的问题。为了预防超急性排斥（HAR）和急性体液异种移植排斥（AHXR），在马萨诸塞州综合医院（Boston，USA）已经产生α 1，3-半乳糖基转移酶基因敲除（GalT-KO）猪。在从GalT-KO猪到狒狒的异种心脏移植中，异位心脏移植物可在179天（中位数78天）内在腹腔内搏动。在异种肾移植中，在慢性免疫抑制方案中，GalT-KO肾移植物在34天前发生排斥反应，但在耐受诱导方案中，移植物可以存活长达83天。GalT-KO心脏和肾脏异种移植物未发生超急性排斥反应。然而，慢性免疫抑制组的所有GalT-KO心脏和肾脏移植物均被AHXR排斥，伴血栓性微血管病。还发生了急性细胞异种移植排斥反应（ACXR）和慢性排斥反应。而在耐受诱导方案的移植肾中，未检测到AHRX、ACXR和慢性排斥反应。因此，我们得出结论，耐受诱导方案是成功的心脏和肾脏移植从GalT-KO猪到狒狒。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Beneficial effects of perioperative low-dose inhaled carbon monoxide on pulmonary allograft survival in MHC-inbred CLAWN miniature swine.

围手术期低剂量吸入一氧化碳对 MHC 近交 CLAWN 小型猪肺同种异体移植物存活的有益影响。

DOI：
10.1097/tp.0b013e3181ff8730
发表时间：
2010
期刊：
Transplantation
影响因子：
6.2
作者：
Sahara,Hisashi;Shimizu,Akira;Setoyama,Kentaro;Oku,Manei;Okumi,Masayoshi;Nishimura,Hiroaki;Oriyanhan,Wunimenghe;Tasaki,Masayuki;Scalea,Joseph;Wada,Hiromi;Bando,Toru;Date,Hiroshi;Yamada,Kazuhiko
通讯作者：
Yamada,Kazuhiko

Prolonged survival of GalT-KO swine skin on baboons.