ヘリコバクター感染による胃粘膜萎縮・腸上皮化生・胃癌への進展とテロメラーゼ活性

螺杆菌感染引起的胃粘膜萎缩、肠上皮化生、胃癌进展和端粒酶活性

• 批准号: 08670605
• 负责人: 渡辺 直樹
• 金额: $ 1.34万
• 依托单位: Sapporo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670605/
• 关键词: H.pylori; テロメア; テロメラーゼ; 胃癌; 腸上皮化生; 萎縮性胃炎; TRAP; ヘリコバクター; 胃粘膜萎縮

不死化した癌細胞や生殖細胞に発現しているテロメラーゼが、癌診断への応用や癌治療の標的分子として、最近注目されている。しかし、良性疾患や前癌病変におけるテロメラーゼ活性の発現については、いまだ不明な点が少なくない。そこで今回、我々は、胃癌患者27例の手術材料における癌および非癌組織のテロメラーゼ活性値を定量化(TPG;Total Product Generated)し、各種背景因子との関係について比較検討した。その結果、(1)癌組織におけるTPGは33.7±29.5と、腸上皮化生(16.7±12.8)、萎縮性胃炎(10.6±9.2)、正常粘膜(3.5±2.6)に比べ、有意に高値を示した。(2)癌組織のTPGと腫瘍径、壁深達度、リンパ節転移の有無、総合的進行程度には、相関関係はみられなかった。(3)一方、H.pylori感染群や分化型胃癌群で、TPGが高い傾向が認められた。とくに、分化型胃癌症例において、前癌病変である腸上皮化生に着目すると、H.pylori感染群のTPGは20.1と、非感染群の1.3に比べ明らかに高値を示した。現在、テロメラーゼRNA(hTR)についても検討を進めている。

Immortalized cancer cells, reproductive cells, cancer cells, reproductive cells, reproductive cells, cancer cells, reproductive cells, reproductive cells, cancer cells, cancer cells, reproductive cells, reproductive cells, cancer cells, reproductive cells, reproductive cells, cancer cells, reproductive cells, cancer cells, cancer cells, reproductive cells, reproductive cells, cancer cells, reproductive cells, reproductive cells, cancer cells, reproductive cells, reproductive cells, reproductive Pre-cancerous diseases, benign diseases, cancers, benign diseases, pre-cancerous diseases, benign diseases, precancerous diseases, benign diseases, precancerous lesions, precancerous diseases, precancerous lesions, precancerous lesions. In this study, 27 patients with gastric cancer were enrolled in this study, and 27 patients with gastric cancer were enrolled in this study. non-cancer tissue, non-cancer tissue, breast cancer, non-cancer tissue, non-cancer tissue, The results were as follows: (1) the TPG of cancer tissue, epithelial metaplasia, atrophic gastritis and normal mucosa were 33.7 ±29.5, 16.7 ±12.8, 10.6 ±9.2 and 3.5 ±2.6 respectively. (2) the diameter of TPG, the depth of the wall, the degree of migration, the degree of progression and the degree of progression of the tumor tissue. (3) one side, H.pylori infection group, differentiated gastric cancer group and TPG infection group. Cases of differentiated gastric cancer, precancerous lesions, epithelial metaplasia, H.pylori infection group, TPG infection group, non-infection group and non-infection group were significantly higher than that of normal group. At present, we are in the process of making progress in the RNA (hTR).

项目成果

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Sugiyama T: "Relationship of Helicobacter pylori to gastric carcinogenesis." Ann Cancer Res Ther. 4. 77-81 (1996)

Sugiyama T：“幽门螺杆菌与胃癌发生的关系。”

Hayashi,S: "A rapid and simple method to quantitate Helicobactor pylori adhesion to humangastric MKN-28 cells." J Gastroenterol Hepatol. 12. 373-375 (1997)

Hayashi,S：“一种快速、简单的方法来定量幽门螺杆菌对人胃 MKN-28 细胞的粘附。”