Structural Change and Activity Control of Peptides and Proteins in Biomembranes Including Boundary Lipids

生物膜中肽和蛋白质（包括边界脂质）的结构变化和活性控制

• 批准号: 10660122
• 负责人: MATSUMURA Yasuki
• 金额: $ 2.11万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10660122/
• 关键词: boundary lipids; biomembranes; proteins; enzymes; structural change; activity control; ペプチド

Acidic phospholipids such as phophatidic acid (PA) and Phosphatidylinositol (PI) and Sphingomyelin (SpM) are believed to act as "boundary lipids" via strong interaction with proteins in biomembranes. These lipids also affect the orientation of phosphatidylcholine (PC) in biomembranes. The purpose of this project is to understand the effects of "boundary lipids" on the structure and activity of peptides and proteins in biomembranes.In this project, lipoxygenase (LOX) was chosen as a target protein, because this enzyme is known to act at the interface of water and lipid. The effects of addition of boundary lipids on the activity of LOX at the interfaces of emulsion droplets and vesicles were investigated. The activity of LOX was increased dramatically by the addition of PA and fatty acids in vesicle dispersions as well as in emulsions. The kinetic analyses and ζ potential measurements demonstrated that the elevation of LOX activity was due to the increase of adsorptivity of LOX to interfaces through electrostatic interaction. Analyses by dynamic light scattering, electron micrograph and ^<31>P-NMR revealed that the addition of PA and fatty acids induced the formation of hexagonal structure and micellar particles from PC vesicle membranes. These results have shown that "boundary lipids" control the enzymatic activity through modification of membrane structure.

酸性磷脂如磷脂酸（PA）和磷脂酰肌醇（PI）和鞘磷脂（SpM）被认为通过与生物膜中的蛋白质的强相互作用而充当“边界脂质”。这些脂质也影响生物膜中磷脂酰胆碱（PC）的取向。本项目的目的是了解“边界脂质”对生物膜中肽和蛋白质的结构和活性的影响，在本项目中，选择脂氧合酶（LOX）作为目标蛋白，因为已知该酶作用于水和脂质的界面。研究了边界脂的加入对脂氧合酶活性的影响。脂氧合酶的活性显着增加PA和脂肪酸在囊泡分散体以及在乳液中。动力学分析和电位测量表明，LOX活性的提高是由于静电作用增加了LOX在界面上的吸附能力。动态光散射、电子显微镜和~ 1H-NMR分析<31>表明，PA和脂肪酸的加入诱导了PC囊泡膜的六方结构和胶束颗粒的形成。这些结果表明，“边界脂质”通过修饰膜结构来控制酶活性。

项目成果

Y.MIZUTANI, Y.MATSUMURA, K.MATSUI, K.CHIBA and T.MORI: "Factors affecting reaction of cucumber root lipoxygenase in phospholipid vesicle dispersions."Biochim.Biophys.Acta. in press. (2001)

Y.MIZUTANI、Y.MATSUMURA​​、K.MATSUI、K.CHIBA 和 T.MORI：“影响磷脂囊泡分散体中黄瓜根脂氧合酶反应的因素”。Biochim.Biophys.Acta。

Y.MATSUMURA, N.MATSUO, J.KIMATA, K.MATSUI and T.MORI: "Reactions of lipoxygenase from cucumber cotyledon in oil-in-water emulsions."Hydrocolloids-part 2 : Fundamentals and Applications in Food, Biology, and Medicine. (ed. by K.NISHINARI) Elsevier (Amsterd

Y.MATSUMURA​​、N.MATSUO、J.KIMATA、K.MATSUI 和 T.MORI：“水包油乳液中黄瓜子叶脂氧合酶的反应”。亲水胶体 - 第 2 部分：食品、生物学和生物医学领域的基础知识和应用

Y.SAKA, T.MORI and Y.MATSUMURA: "Reaction of soybean lipoxygenase-3 in emulsions as affected by emulsifiers, salts and phospholipids."Colloid Surfaces B : Biointerfaces. 19. 187-196 (2000)

Y.SAKA、T.MORI 和 Y.MATSUMURA​​：“乳液中大豆脂氧合酶 3 的反应受乳化剂、盐和磷脂的影响。”胶体表面 B：生物界面。

Y.Saka: "Reaction of Soybean Liponygenase-3 in Emulsions as Affected by Emulsifiers,Salts and Phospholipids"Collids and Surface (B) : Biointerfaces. 14(in press). (2000)

Y.Saka：“受乳化剂、盐和磷脂影响的乳液中大豆脂酶 3 的反应”胶体和表面 (B)：生物界面。

Y.Saka,T.Mori,Y.Matsumura: "Recation of soybean lipoxygenase-3 in emulsions as affected by emulsifiers, salts and phospholipids"Colloid Surfaces B : Biointerfaces. 19. 187-196 (2000)

Y.Saka、T.Mori、Y.Matsumura：“受乳化剂、盐和磷脂影响的乳液中大豆脂氧合酶 3 的再活化”胶体表面 B：生物界面。