Development of rapid detection method of lymph node metastasis from prostate cancer

前列腺癌淋巴结转移快速检测方法的研制

• 批准号: 13671644
• 负责人: TAKAHASHI Yoshito
• 金额: $ 1.86万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671644/
• 关键词: RT-PCR method; lymph node metastasis; molecular diagnosis; prostate cancer; PSA; RT-PCR(定量的); 転移

The radical prostatectomy is the main therapy of prostate cancer.Though the evaluation before prostatectomy reveals no remarkable extra-prostatic metastasis, recurrence or metastasis occurs in some cases after prostatectomy.The high sensitive detection of micro-metastasis needs for clinically.Therefore, we developed detection method of micro-metastasis using RT-PCR method. This method detects PSAmRNA, needs a little specimen, but the duration of reaction is too long for rapid diagnosis. And this method has high sensitivity, but no quantity. We developed rapid diagnostic method using PCR in capillary. The duration of reaction of new method only 30 minutes. And we developed quantitative detection of prostatic cancer cell in the serum.These new method contributes clinical aspect of prostatic micro-metastasis.

前列腺癌的主要治疗方法是前列腺癌根治术，虽然前列腺切除前的评估未发现明显的前列腺外转移，但前列腺切除后仍有部分病例出现复发或转移。临床需要高灵敏度的微转移检测。为此，我们开发了采用RT-PCR方法检测微转移的方法。该方法检测PSAmRNA，需要少量标本，但反应时间过长，不利于快速诊断。而且这种方法灵敏度高，但没有定量。我们开发了在毛细管中使用 PCR 的快速诊断方法。新方法的反应时间仅需30分钟。并开发了血清中前列腺癌细胞的定量检测方法。这些新方法为前列腺微转移的临床研究做出了贡献。

项目成果

出口隆, 江原英俊, 高橋義人: "前立腺癌 残存癌と微少転移の診断法、RT-PCR法によるPSAmRNAおよびPSAmRNAの検出"日本臨床. 60. 151-155 (2002)

Takashi Deguchi，Hidetoshi Ebara，Yoshito Takahashi：“前列腺癌残留癌和微转移的诊断方法，通过RT-PCR方法检测PSA mRNA和PSA mRNA”日本临床研究60. 151-155（2002）。

Takashi Deguchi, Hidetoshi Ehara and Yoshito Takahashi: "Prostate cancer; Diagnosis of remained cancer and micro-metastasis Detection of PSAmRNA and PSMAmRNA using RT-PCR method"Nihon rinshoh. vol.60. 151-155 (2002)

Takashi Deguchi、Hidetoshi Ehara 和 Yoshito Takahashi：“前列腺癌；残留癌的诊断和微转移使用 RT-PCR 方法检测 PSAmRNA 和 PSMAmRNA”Nihon rinshoh。