Investigation of mechanism of connective tissue growth factor (CTGF) as a molecular target against cancer-induced bone destruction
结缔组织生长因子(CTGF)作为对抗癌症引起的骨破坏的分子靶标的机制研究
基本信息
- 批准号:13672093
- 负责人:
- 金额:$ 2.3万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2001
- 资助国家:日本
- 起止时间:2001 至 2002
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Osteoclastic bone resorption plays an important role on cancer-induced bone disease likes bone metastases or cancer bone invasion. We previously reported that administration of antibody against connective tissue growth factor (CTGF) inhibited osteolytic lesions in nude mice bone metastasis model. It is suggested that inhibition of CTGF has a possibility of therapeutic use for the treatment of the cancer induced bone disease. However, the relationship between osteoclastic bone resorption and CTGF has been still unknown. In the present study, we examined the mechanism of CTGF on osteoclastic bone resorption to know whether we could use CTGF as a molecular target against the cancer-induced bone disease.Immunohistologically, the expression of CTGF protein was observed at bone-marrow stromal cells and osteoclasts, and especially localized at cytoplasm around the nuclei of osteoclasts in murine bone marrow culture system in vitro. CTGF anti-sense oligonucleotide (AS-CTGF) suppressed osteocla … More st formation, but did not inhibit mature osteoclastic bone resorption on dentin slice in vitro. AS-CTGF suppressed the expression of ODF (RANKL) mRNA in bone marrow /stromal cell line ST2, but did not affect the OCIF mRNA expression. Next, we examined the bone metabolism in nude mice subcutaneous implanting CHO cells transfected with CTGF gene. In spite of over-production of CTGF, systemic hypercalcemia was not observed in nude mice. And there is no difference of the tumor growth and body weight loss between the nude mice transfected with the transfectants and non-transfectant.It is well known that many growth factors store in bone matrices. Release of these growth factors like TGF-β by cancer-induced bone resorption probably regulates the biological events in the bone microenvironment. TGF-β dose-dependently increased the expression of CTGF mRNA of not only human breast cancer cells line MDA-231, but also ST2 cell. This indicates that TGF-β might regulate the CTGF expression of local bone resorption sites as paracrine factor. From these results, CTGF may play an important role on the local bone resorption site, and the inhibition of CTGF expression may be useful therapy for local bone destruction induced by cancer. Less
破骨细胞骨吸收在骨转移或癌骨侵袭等癌症引起的骨病中发挥着重要作用。我们之前报道过给予结缔组织生长因子(CTGF)抗体可以抑制裸小鼠骨转移模型中的溶骨性病变。提示抑制CTGF可能用于治疗癌症引起的骨疾病。然而,骨钙素骨吸收与CTGF的关系尚不清楚。本研究通过免疫组织化学方法观察CTGF在体外培养的小鼠骨髓基质细胞和破骨细胞中的表达,并以破骨细胞核周围的胞浆表达为主,以探讨CTGF对骨吸收的影响机制。CTGF反义寡核苷酸抑制骨细胞凋亡 ...更多信息 在体外牙本质切片上,ST形成,但不抑制成熟骨细胞的骨吸收。AS-CTGF抑制骨髓基质细胞系ST 2中ODF(RANKL)mRNA的表达,但不影响OCIF mRNA的表达。接下来,我们研究了裸鼠皮下植入转染CTGF基因的CHO细胞的骨代谢。尽管CTGF过量产生,但在裸小鼠中并未观察到全身性高钙血症。转染后裸鼠的肿瘤生长和体重减轻与未转染的裸鼠无明显差异。通过癌症诱导的骨吸收释放这些生长因子如TGF-β可能调节骨微环境中的生物学事件。TGF-β浓度依赖性地增加人乳腺癌细胞株MDA-231和ST 2细胞CTGF mRNA的表达。提示TGF-β可能作为旁分泌因子调节骨吸收局部CTGF的表达。因此,CTGF可能在局部骨吸收位点发挥重要作用,抑制CTGF表达可能是治疗肿瘤引起的局部骨破坏的有效方法。少
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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SASAKI Akira其他文献
Comparative Studies of Learning Effect Among Teacher Trainee Students who have Participated in Different Form of School Volunteers
参加不同形式学校志愿者的见习教师学生学习效果比较研究
- DOI:
- 发表时间:
2007 - 期刊:
- 影响因子:0
- 作者:
UMEZAWA Minoru;SASAKI Akira;SHONO Yumi;KONDO Keiko;KINOSHITA Koji;HORIE Mariko and FUJIWARA Nobuhiko;UMEZAWA Minoru;UMEZAWA Minoru;UMEZAWA Minoru;HIMENO Kanji - 通讯作者:
HIMENO Kanji
A Study about Communication of a Teacher by Childcare and an infant: Based on a Communication Example in a Kindergarten
保育教师与幼儿的沟通研究——基于幼儿园沟通实例
- DOI:
- 发表时间:
2007 - 期刊:
- 影响因子:0
- 作者:
UMEZAWA Minoru;SASAKI Akira;SHONO Yumi;KONDO Keiko;KINOSHITA Koji;HORIE Mariko and FUJIWARA Nobuhiko;UMEZAWA Minoru;UMEZAWA Minoru - 通讯作者:
UMEZAWA Minoru
Developing the Students' Power of Observation Based on Child-Care Worker's 'Narrative': Introduction of 'Narrative' Case Study in the Kindergarten attached to Naruto University of Education
基于保育员“叙事”培养学生观察力——鸣门教育大学附属幼儿园“叙事”案例介绍
- DOI:
- 发表时间:
2007 - 期刊:
- 影响因子:0
- 作者:
UMEZAWA Minoru;SASAKI Akira;SHONO Yumi;KONDO Keiko;KINOSHITA Koji;HORIE Mariko and FUJIWARA Nobuhiko - 通讯作者:
HORIE Mariko and FUJIWARA Nobuhiko
How does a Student Teacher Observe a Class?
实习老师如何听课?
- DOI:
- 发表时间:
2007 - 期刊:
- 影响因子:0
- 作者:
UMEZAWA Minoru;SASAKI Akira;SHONO Yumi;KONDO Keiko;KINOSHITA Koji;HORIE Mariko and FUJIWARA Nobuhiko;UMEZAWA Minoru - 通讯作者:
UMEZAWA Minoru
Problem to Create Study Activity in Class of Reading: The Symbol Principle and the Situation Theory of the Cognitive Science are Invoked
阅读课创设学习活动的问题:援引认知科学的符号原理和情境理论
- DOI:
- 发表时间:
2007 - 期刊:
- 影响因子:0
- 作者:
UMEZAWA Minoru;SASAKI Akira;SHONO Yumi;KONDO Keiko;KINOSHITA Koji;HORIE Mariko and FUJIWARA Nobuhiko;UMEZAWA Minoru;UMEZAWA Minoru;UMEZAWA Minoru - 通讯作者:
UMEZAWA Minoru
SASAKI Akira的其他文献
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{{ truncateString('SASAKI Akira', 18)}}的其他基金
The relationship of hearing impairment and systemic disease: an epidemiological study
听力障碍与全身性疾病的关系:流行病学研究
- 批准号:
24791737 - 财政年份:2012
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Evaluation of gene delivery mechanism based on quantitative visualization of cellular defense system against exogenous genes for high-efficiency gene therapy
基于针对外源基因的细胞防御系统定量可视化的基因传递机制评估,以实现高效基因治疗
- 批准号:
24790036 - 财政年份:2012
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Investigation of phase transition processes arises from atomic and radiative property of plasmas for EUV and X-ray laser applications
对 EUV 和 X 射线激光应用中等离子体的原子和辐射特性进行相变过程的研究
- 批准号:
23340185 - 财政年份:2011
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
A Research of an Implementation Method of Domain Specific Languages Based on Incremental Extention
基于增量扩展的领域专用语言实现方法研究
- 批准号:
23700043 - 财政年份:2011
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Analysis of the low invasive surgical treatment effect and dynamic state of adipose cell origin hormone of morbid obesity patients
病态肥胖患者低创手术治疗效果及脂肪细胞来源激素动态分析
- 批准号:
22591440 - 财政年份:2010
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
A Study for Supporting Integration of Knowledge across Interdisciplinary Fields using Domain Specific Languages
使用领域特定语言支持跨学科领域知识整合的研究
- 批准号:
21700041 - 财政年份:2009
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Epidemics and evolution on the commuter network in metropolitan area
都市圈通勤网络的流行与演变
- 批准号:
21370009 - 财政年份:2009
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Investigation of mechanism of angiogenesis factor, angiogenin, as a molecular target against cancer-induced bone destruction
研究血管生成因子血管生成素作为对抗癌症引起的骨破坏的分子靶标的机制
- 批准号:
20390516 - 财政年份:2008
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of an integrated model of the hybrid pumping plasma light source based on laser assisted discharge
基于激光辅助放电的混合泵浦等离子体光源集成模型的研制
- 批准号:
20340166 - 财政年份:2008
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
The relationship of hearing impairment and systemic disease : anepidemiological study
听力障碍与全身性疾病的关系:流行病学研究
- 批准号:
19890013 - 财政年份:2007
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Young Scientists (Start-up)
相似海外基金
Connective Tissue Growth Factor (CTGF): A key player in Fibrosis and Cancer
结缔组织生长因子 (CTGF):纤维化和癌症的关键参与者
- 批准号:
443371 - 财政年份:2021
- 资助金额:
$ 2.3万 - 项目类别:
Operating Grants
Regulation of the pro-fibrotic connective tissue growth factor in alcoholic liver disease: mechanisms and targeting approaches
酒精性肝病中促纤维化结缔组织生长因子的调节:机制和靶向方法
- 批准号:
10554401 - 财政年份:2020
- 资助金额:
$ 2.3万 - 项目类别:
Regulation of the pro-fibrotic connective tissue growth factor in alcoholic liver disease: mechanisms and targeting approaches
酒精性肝病中促纤维化结缔组织生长因子的调节:机制和靶向方法
- 批准号:
10419156 - 财政年份:2020
- 资助金额:
$ 2.3万 - 项目类别:
Regulation of the pro-fibrotic connective tissue growth factor in alcoholic liver disease: mechanisms and targeting approaches
酒精性肝病中促纤维化结缔组织生长因子的调节:机制和靶向方法
- 批准号:
10356797 - 财政年份:2020
- 资助金额:
$ 2.3万 - 项目类别:
Regulation of the pro-fibrotic connective tissue growth factor in alcoholic liver disease: mechanisms and targeting approaches
酒精性肝病中促纤维化结缔组织生长因子的调节:机制和靶向方法
- 批准号:
10090543 - 财政年份:2020
- 资助金额:
$ 2.3万 - 项目类别:
Connective tissue growth factor: an intriguing therapeutic target in alcoholic liver disease
结缔组织生长因子:酒精性肝病的一个有趣的治疗靶点
- 批准号:
9210038 - 财政年份:2016
- 资助金额:
$ 2.3万 - 项目类别:
The central role of Connective Tissue Growth Factor in remodelling of asthmatic airways
结缔组织生长因子在哮喘气道重塑中的核心作用
- 批准号:
nhmrc : 1061712 - 财政年份:2014
- 资助金额:
$ 2.3万 - 项目类别:
Project Grants
Significance of connective tissue growth factor (CTGF) and tumor-stroma interaction in Kras-driven hepatocarcinogenesis and HCC progression
结缔组织生长因子 (CTGF) 和肿瘤间质相互作用在 Kras 驱动的肝癌发生和 HCC 进展中的意义
- 批准号:
26670381 - 财政年份:2014
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Antifibrotic effect and role of antibody to connective tissue growth factor in an animal model of scleroderma
结缔组织生长因子抗体在硬皮病动物模型中的抗纤维化作用和作用
- 批准号:
24890076 - 财政年份:2012
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$ 2.3万 - 项目类别:
Grant-in-Aid for Research Activity Start-up
A search and evaluation of the peptides that control the connective tissue growth factor for the prevention and the treatment of osteoarthritis.
寻找和评估控制结缔组织生长因子的肽,以预防和治疗骨关节炎。
- 批准号:
24792142 - 财政年份:2012
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Young Scientists (B)